Laboratory guidelines

Laboratory workshop, Barcelona, 9th March 2016

Presentation

Dr. Carmen Muñoz Almagro (Laboratory focal point) has developed in collaboration with Benoit Garin and Sophie Guillot from Institut Pasteur, laboratory recommendations for PERTINENT partners. The recommendations are based on the ECDC, CDC and WHO guidelines and on a laboratory survey conducted among PERTINENT partners (see Annex on laboratory guidelines of the generic protocol).

These recommendations were the basis of the one-day training, held in Barcelona 9th March, on pertussis laboratory diagnosis for microbiologists participating in PERTINENT.

Documentation

Following documentation is available for download at the bottom of the page:

• Workshop agenda

• Workshop lectures

  1. Specimen collection, transport and extraction; Didrik F. Vestrheim

  2. Culture based techniques and isolate conservation; Sophie Guillot

  3. Serological diagnosis and vaccination in maternal age; Alex-Mikael Barkoff

  4. Molecular based techniques; Carmen Muños-Almagro

• Laboratory guidelines for PERTINENT

• Nasopharyngeal aspirate documentation

• References:

• • • WHO Laboratory Manual for the diagnosis of whooping cough caused by Bordetella pertussis/Bordetella parapertussis

    • ECDC Technical document: Guidance and protocol for the use of RT-PCR in laboratory diagnosis of human infection with Bordetella pertussis/Bordetella papapertussis

    • 2011 Journal of Clinical Micrbiology, Novel multitarget Real-Time PCR assay for rapid detection of Bordetella Species in clinical specimens