Standard Operating Procedure

STANDARD OPERATING PROCEDURE(SOP)

Alternative Energy Laboratory

Department of Petroleum Studies

AMU Aligarh

Location of SOP:

Website of Alternative Energy Laboratory and hardcopy in the Alternative Energy Laboratory.

Before working in the lab:

· You must have permission from Dr. Moina Athar.

· You must have received formal training from technician or, trained research student (designated by Dr. Moina Athar) related to research equipment safety and operation.

· You must read and understand SOP.

· You must use all the facilities under direct supervision of Dr. Moina Athar or, trained research student (designated by Dr. Moina Athar).

· If you do NOT follow above instructions you will be held responsible for your own safety and damages.

Safety Precautions:

· Students without shoes will not be allowed in the lab.

· Bring lab coat and a permanent marker to lab.

· Always wear full sleeves clothes.

· Wear safety glasses and gloves when recommended.

· Leave bags and coats in designated areas.

· Bring only the essentials to the lab bench.

· No eating, drinking, playing, or applying cosmetics (including hand lotion, etc.).

· Never use broken or chipped glassware.

· Mouth pipetting is forbidden.

· Hands should be washed after contact with hazardous materials and before leaving the lab.

Lab Etiquette:

Return all chemicals and supplies to the proper location after use. It is necessary to take chemicals from reagent bottles, pour out slightly more than the amount of chemical needed into a clean

beaker. Never pour a chemical back into a reagent bottle. Clean up for the next person. At the

conclusion of each work period, all used glassware must be cleaned and set to drain. Remove label

tape, scrub inside of glassware with water and laboratory detergent, rinse with tap water, rinse with

distilled water, and place cleaned glassware on a rack to dry. No experiment is complete until the

laboratory is cleaned.

Procedure for Cleaning Laboratory Glassware

Cleaning

Ø Remove all labels off glassware using a sponge or acetone.

Ø Wash with a laboratory detergent using a brush to scrub inside of the glassware, stopcocks, and other small pieces. This is a good step to use if your glassware contains any particulates.

Ø For removing organic compounds, it may be useful to use one of the following cleaning procedures:

• A thorough rinse with pesticide grade acetone, methanol or hexane

• Soaking in an oxidizing agent (Chromic acid or equivalent)

• Soaking in a mixture of HCl : HNO3 (3:1 v/v)

Ø For removing inorganic compounds, it may be useful using also one of the following cleaning procedures:

• Rinsing or soaking with HCl : H2O (1:1 v/v)

• Rinsing or soaking with >10% HNO3

• Soaking with a mixture of HCl : HNO3 (3:1 v/v)

• Soaking in a mixture of sulfuric acid and hydrogen peroxide (H2SO4 and H2O2)

Ø Rinse thoroughly with hot tap water three times.

Ø Rinse thoroughly with de-ionized water three times.

Ø (Note: New glassware must be soaked overnight in 10% HNO3 or HCl. The analyst needs to determine which washing treatment would be most efficient for her glassware.)

Drying

Ø Allow glassware to thoroughly dry in the drying rack. If dry glassware is urgently needed, it may be dried in an oven at 60°C.

Ø Store glassware carefully during drying operations. Do not place round or oddly shaped glassware on countertops or other flat surfaces where they might roll off and break. Drying racks are the best option for most glassware. However, ensure that the glassware is adequately supported on the rack.

Ø Never heat volumetric class A glassware.

Storage

Clean dry glassware should be sealed and stored in dust-free environment