1) Live cell imaging of mitochondria in human iPSC-derived Fragile X Syndrome neurons 4 weeks after plating for terminal differentiation using JC10 dye. Blue: live cell nuclear dye; Green and red: JC10 dye 

Instrument Used: Nano high content imaging system at 20x magnification. 

Carissa Sirois

2) Drosophila melanogaster testis filled with individualizing GFP-labeled sperm. Sub-optimal warm temperature disrupts sperm individualization and development, abruptly decreasing sperm abundance and quality. DAPI (white), nuclei; phalloidin (magenta), actin; GFP (green), Protamin B (labels germ cell nuclei starting in late canoe stage) and Don Juan (labels sperm tails). Scale bar = 20 μm. 

Instrument Used: LSM 900 confocal system using the integrated Airyscan 2 detector and corresponding Airyscan SR mode (ZEISS Microscopy) and processed with the standard Airyscan Processing utilities of the ZEN Blue 3.5 sofware (ZEISS Microscopy) to achieve super-resolution readouts. 

Ana Caroline Gandara

3) Image of murine chondrocytes (red) expressing signaling molecular, Ihh (teal) in an ulnar 10 days post fracture callus. 40X. Hox11 MSCs differentiate to either osteoblasts, chondrocytes, or adipocytes during development and in response to injury. 

Katherine Hubert

4) An adult human fibroblast was transduced with two transcription factors that directly reprogram it into a glutamatergic neuron. Here you can see the membrane remodeling of the distal regions of the fibroblast into neurite morphology, while the cell body still looks like a fibroblast.

Darcie Moore

5) Human iPSC-derived cortical excitatory neuron captured at 63X using a confocal microscope. Pictured neuron was infected with a lentiviral vector carrying an mCherry expression plasmid, grown for 6 weeks after terminal differentiation, and fixed. Coverslips were immunolabeled for synapsin-1 (green) to identify presynaptic terminals and counterstained with DAPI (blue) to label all nuclei.

Matt Russo

6) A Regeneration Time Course of Beta Cell Identity Insulin, shown in red, identifies beta cells within pancreatic islets and Urocortin3, shown in green, reveals the maturity of each beta cell.

Instrument Used: Keyence BZ-X800 using a 20X apochromat lens.

Matthew Wagner

7) Human pluripotent stem cells on micropatterned culture surfaces spontaneously self-assemble to form neural rosettes tissues. Shown here at 60X magnification confocal imaging, rosette tissues involve cell proliferation, migration, and differentiation.

While most tissues organize into a stereotypical configuration (center), others were destined for different "fates". (N-cadherin - green, Pax6-red, DAPI-blue, ~200 μm diameter)

Brady Lundin