1) Autoflourescent imaging of NADPH and FAD and calculated ORR of human T cell subsets isolated from mice developing xenogeneic GVHD

Nicholas Hess and Jeremiah Riendeau

2) Photoreceptors Seeded In Honeycomb Scaffolds: Immunocytochemistry of photoreceptor cells seeded in the honeycomb patterned scaffolds, where Recoverin and CRX proteins are labeled with green and red, respectively.

Instrument Used: Nikon Eclipse Ti2, Widefield Fluorescent, Z-Stack 50 optical Sections, Maximum Intensity Projection, Plan Apo 40X

Jiahe Jin

3) Better luck to-marrow: Adult mouse ulnar bone 10 days after fracture. Stem cells (green) are differentiating into bone (red) to repair the site of fracture. 20x stitched images taken on Keyence microscope.

Instrument Used: Keyence Microscope

Katherine Hubert

4) Description not provided

Daniel Osorio-Mendez

5) Hoxa5eGFP localization in the respiratory system of a mouse embryo: A frontal section of a Hoxa5eGFP/+ embryo at E12.5 reveals that Hoxa5eGFP is localized at the trachea, main bronchi, lung, scapulae, and diaphragm. The lung looks like a large walnut!

Muhang Li

6) Hematopoietic stem cell niche in a dish: Network of endothelial cells (Red) with perivascular attachment of mesenchymal stromal cells (green) support hematopoietic stem cells (white) in vitro and mimic the endogenous Hematopoietic stem cell niche.

Instrument Used: ImageXpress Nano High-Content Imaging System

Mariyam Akter

7) Hox11 lineage labeled myoculei: Using a nuclear lineage tracing system specific to Hox11 we observe that Hox11 lineage labeled nuclei contribute to muscle cells in adult mice. This surprising finding suggests there are additional cell populations contributing to adult muscle other than muscle stem cells.

Instrument used: Keyence BZ-X800

Corey Flynn

8) Description not provided

Nicole Woodhead

9) Seen are the branches of the coronary arteries in the heart. This was visualized by an arterial cell reporter model and captured in whole-mount using a confocal microscope

Rebecca Salamon

10) Transitions: An image from the olfactory bulb of a mouse showing newly born cells (magenta) originating from distal neural stem cells in the subventricular zone. Also shown is labeling for Tgfbr2, and the two labels form an apparent gradient moving from the interior of the olfactory bulb on the left to the exterior on the right.

Instrument Used: Leica SP8 laser scanning confocal microscope and LasX software at the School of Veterinary Medicine.

Daniel Radecki