Publications

Zhang, W., Ntai, I., Kelleher, N. L., Walsh, C. T. “tRNA-dependent peptide bond formation by the transferase PacB in biosynthesis of the pacidamycin group of pentapeptidyl nucleoside antibiotics.” Proc. Natl. Acad. Sci. U. S. A. 2011, 108, 12249-12253. Link

Zhang, W., Ames, B. D., Walsh, C. T. “Identification of phenylalanine 3-hydroxylase for meta-tyrosine biosynthesis.” Biochemistry 2011, 50, 5401-5403. Link

Zhang, W., Ntai, I., Bolla, M. L., Malcolmson, S. J., Kahne, D., Kelleher, N. L., Walsh, C. T. “Nine enzymes are required for assembly of the pacidamycin group of peptidyl nucleoside antibiotics” J. Am. Chem. Soc. 2011, 133, 5240-5243. Link

Zhang, W., Heemstra, J. R., Walsh, C. T., Imker H. J. “Activation of the pacidamycin PacL adenylation domain by MbtH-like proteins” Biochemistry 2010, 49, 9946-9947. Link

Zhang, W., Ostash, B., Walsh, C. T. “Identification of the biosynthetic gene cluster for the pacidamycin group of peptidyl nucleoside antibiotics” Proc. Natl. Acad. Sci. U. S. A. 2010, 107, 16828-16833. Link

Zhang, W., Bolla, M. L., Kahne, D., Walsh, C. T. “A three enzyme pathway for 2-amino-3-hydroxycyclopent-2-enone formation and incorporation in natural product biosynthesis” J. Am. Chem. Soc. 2010, 132, 6402-6411. Link

Yan, M., Du, J., Gu, Z., Liang, M., Hu, Y.,Zhang, W., Priceman, S., Wu, L., Segura, T., Liu, Z., Tang, Y., Lu, Y. “A novel protein delivery platform based on single protein nanocapsules.” Nat. Nanotech. 2010, 5, 48-53. Link

Zhang, W., Tang, Y.  “In vitro Analysis of Type II PKS” Methods in Enzymology: microbial natural product biosynthesis, 2009, 459, 367-393. Link

Zhang, W., Ferreira, . P., Tang, Y. "Regulation of Secondary Metabolism Biosynthesis" In Smolke, C.D. (Ed.) The Metabolic Pathway Engineering Handbook: Tools and Applications. CRC Press. 2009. Link

Gao, X., Xie, X., Pashkov, I., Sawaya, R. M., Laidmen, J., Zhang, W., Cacho, R., Yeates, T. O., Tang, Y. “Directed evolution and structural characterization of a simvastatin synthase.” Chem. Biol. 2009, 16, 1064-1074. Link

Wang, P.*,Zhang, W.*, Zhan, J., Tang, Y. “Identification of OxyE as an ancillary oxygenase during tetracycline biosynthesis.” ChemBioChem, 2009, 10, 1554-1550. (*Equal contribution) Link

Zhang, W., Li, Y., Tang, Y. “Engineered biosynthesis of bacterial aromatic polyketides in Escherichia coli” Proc. Natl. Acad. Sci. U. S. A., 2008, 105, 20683-20685. Link

Zhang, W., Watanabe, K., Cai, X., Jung, M. E., Tang, Y., Zhan, J. “Identifying the minimal enzymes required for anhydrotetracycline biosynthesis” J. Am. Chem. Soc. 2008, 130, 6068-6069. Link

Ames, B., Korman, T.,Zhang, W., Vu, T., Tang, Y., Tsai, S-C. “Crystal structure and functional analysis of tetracenomycin ARO/CYC: implication for cyclization specificity of aromatic polyketides” Proc. Natl. Acad. Sci. U. S. A. 2008, 105, 5349-5354. Link

Zhang, W., Tang, Y. “Combinatorial biosynthesis of natural products” J. Med. Chem. 2008, 51, 2629-2633. Link
Zhu, X., Zhang, W., Xiao, H., Huang, J., Li, G. “Electrochemical study of a hemin-DNA complex and its activity as a ligand binder” Electrochim. Acta 2008, 53, 4407-4413. Link

Ma, S. M.*, Zhan, J., Xie, X., Watanabe, K., Tang, Y., Zhang, W* “Redirecting the cyclization steps of fungal polyketide synthase” J. Am. Chem. Soc. 2007, 130, 38-39.  (*Equal contribution) Link

Zhang, W., Wilke, B. I., Zhan, J., Watanabe, K., Boddy, C. N., Tang, Y. “A new mechanism for benzopyrone formation in aromatic polyketide biosynthesis” J. Am. Chem. Soc. 2007, 129, 9304-9305. Link

Zhang, W., Watanabe, K., Wang, C. C. C., Tang, Y. “Investigation of early tailoring reactions in the oxytetracycline biosynthetic pathway” J. Biol. Chem. 2007, 282, 25717-25725. Link

Ma, S. M., Zhan, J., Watanabe, K., Xie, X., Zhang, W., Wang, C. C. C., Tang, Y. “Enzymatic synthesis of aromatic polyketides using PKS4 from Gibberella fujikuroi” J. Am. Chem. Soc. 2007, 129, 10642-10643. Link

Zhang, W., Watanabe, K., Wang, C. C. C., Tang, Y. “Heterologous biosynthesis of amidated polyketides with novel cyclization regioselectivity from oxytetracycline polyketide synthase” J. Nat. Prod. 2006, 69, 1633-1636. Link

Zhang, W., Tang, Y. "Engineering Starter Units in Aromatic Polyketides" In S. R. Baerson (Ed.), Polyketides: Biosynthesis, Biological Activity and Genetic Engineering. American Chemical Society. 2006. 231-245 Link

Zhang, W., Ames, B., Tsai, S-C., Tang, Y. “Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase” Appl. Environ. Microb. 2006, 72, 2573-2580. Link

Huang, Y., Zhang, W., Xiao, H., Li, G. “An electrochemical investigation of glucose oxidase at a US nanoparticles modified electrode” Biosens. & Bioelectro. 2005, 21, 817-821. Link

Liu, X., Huang, Y., Zhang, W., Fan, G., Fan, C., Li, G. “Electrochemical investigation of redox thermodynamics of immobilized myoglobin: Ionic and ligation effects” Langmuir 2005, 21, 375-378. Link

Zhang, W., Zhou, H., Li, G., Scheer, H. “An electrochemical study of hemoglobin in water-glycerol solutions” Biophys. Chem. 2004, 111, 229-233. Link

Zhang, W., Huang, Y., Dai, H., Wang, X., Fan, C., Li, G. “Tuning the redox and enzymatic activity of glucose oxidase in layered organic films and its application in glucose biosensors” Anal. Biochem. 2004, 329, 85-90. Link

Zhang, W., Li, G. “Third-generation biosensors based on the direct electron transfer of proteins” Anal. Sci. 2004, 20, 603-609. Link

Liu, X., Zhang, W., Huang, Y., Li, G. “Enhanced electron-transfer reactivity of horseradish peroxidase in phosphatidylcholine films and its catalysis to nitric oxide” J. Biotech. 2004, 108, 145-152. Link

Sun, Z., Ma, Z., Zhang, W., Wang, X., Fan, C., Li, G. “Electrochemical investigations of baicalin and DNA-baicalin interactions” Anal. Bioanal. Chem. 2004, 379, 283-286. Link

Sun, Y., Liu, X., Fan, C., Zhang, W., Li, G. “Electrochemical investigation of the chloride effect on hemoglobin” Bioelectrochemistry 2004, 64, 23-27. Link

Zhang, W., Fan, C., Sun, Y., Li, G. “An electrochemical investigation of ligand-binding abilities of film-entrapped myoglobin” Biochim. Biophys. Acta. Gen. Subj. 2003, 1623, 29-32. Link

Peng, W., Liu, X., Zhang, W., Li, G. “An electrochemical investigation of effect of ATP on hemoglobin” Biophys. Chem. 2003, 106, 267-273. Link

Fan, C., Liu, J., Zhang, W., Suzuki, I., Li, G. “Enhanced electron-transfer reactivity of cytochrome b(5) by dimethylsulfoxide and N,N'-dimethylformamide” Anal. Sci. 2002, 18, 1031-1033. Link