1.  S

</> NGS

• RNA sequencing: data QC, DEG, clustering, GO term enrichment, and time-series data analysis (Trinity, Trimmomatic, Cutadapt, FastQC, STAR, HISAT2, RSEM, Kallisto, Cufflinks, Stringtie, edgeR, ClueGO, STEM)

• Library construction for whole-genome sequencing (WGS): long-read sequencing based on the Oxford Nanopore platform, ONT

✂ Gene editing 

• CRISPR binary vector cloning and plant tissue culture in wild tobacco (Nicotiana attenuata)

• Heritable virus-induced gene editing (VIGE)

• Illumina-based amplicon deep sequencing (targeted deep sequencing) for editing efficacy

🧬 Molecular biology

• PCR, RT-qPCR, cloning - ligation, gibson, golden gate assembly

• In situ hybridization (ISH)

• Western blot

• Yeast two-hybrid (Y2H, 예정)

• Protoplast and nuclei isolation for FACs

• Promoter binding assay (예정)

• Virus-induced gene silencing (VIGS) in wild tobacco (N. attenuata)

📊 Metabolite analysis 

• GC-MS (Q) Primary metabolites derivatization, Headspace volatile analysis via PDMS, Analyzing the internal pool of floral volatiles

• LC-MS (예정) Hormone analysis - JA, IAA, ABA

• Chlorophyll quantification

• Starch quantification

🔬 Histology

• Paraffin embedding and section using microtome, cryotome - flower and leaf of wild tobacco (Nicotiana attenuata), Arabidopsis thaliana, and morning glory (Ipomoea purpurea)

• Imaging by slide scanner and confocal microscopy

Feb, 2025  |  Young scientist talk (15 min), Title: Identification of flower opening regulators under circadian clock control, @The 4th MSL Symposium

Nov, 2024  |  Oral (15 min), Title: Identification of flower opening regulators controlled by circadian clock, @Graduate Seminar, KAIST

Nov, 2024  |  Flash talk (4 min) & Poster, Title: Identification of flower opening regulators controlled by circadian clock, @International Conference, Korean Society of Plant Biologists (KSPB)

Aug, 2024  |  Poster, Title: The molecular mechanism underlying the Linneaus's flower clock, @The 100th international Plant Biology Conference, American Society of Plant Biologists (ASPB)

Feb, 2024  |  2 min talk, @The 3rd MSL Symposium

Feb, 2023  |  2 min talk, @The 2nd MSL Symposium

May, 2019  |  Poster, Title: The molecular mechanism underlying the Linneaus flower clock, @Plants in Daejeon (PiD)

Feb, 2019  |  2 min talk, @The 1st MSL Symposium

🥼 Sep. 2018 – present |

Research Assistant, Department of Biological Sciences, KAIST (Daejeon, Republic of Korea)
*Laboratory for Ecology (Advisor: Prof. Sang-Gyu Kim)

*Proj. Flower opening: How do plants with conserved circadian clock bloom at different times?
- Analyzed the time-series RNA sequencing data and primary metabolite data 

*Proj. Flower longevity: How to improve the longevity of flowers?
- Conducted the whole-genome sequencing by Nanopore, Generated the knock-out line 

🥼 Mar. 2017 – Feb. 2018 |

Undergraduate Research Assistant, Department of Horticulture, Chungnam National University (Daejeon, Republic of Korea)
*Molecular Genetics and Genomics Laboratory (Advisors: Prof. Yong Pyo Lim, Dr. Soyoung Yi) 

*Proj. Leaf senescence: What is the molecular mechanism of dark-induced leaf senescence?
- Estimated the chlorophyll content for plant material selection

June, 2017  |  Visiting Student, Plant Systems Engineering Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB; Daejeon, Republic of Korea)

Aug. 2016  |  Visiting Student, Education Training Program of Academy of Korea Agriculture (Netherlands & Belgium)

Mar. – Dec. 2022  |  Korea Science Academy (KSA; gifted high school) Research & Education (R&E) program, Teaching Assistant, KAIST

Sep. 2021 – Feb. 2022  |  General Biology, Teaching Assistant, KAIST

Mar. –  Aug. 2021  |  Scientific Writing in English, Teaching Assistant, KAIST

Jan. – June, 2021  |  Undergraduate Research Program (URP), Teaching Assistant, KAIST

Jul. – Aug. 2019  |  Sejong Academy of Science and Arts (SASA; gifted high school), Teaching Assistant, KAIST

Mar. – Aug. 2019  |  General Biology, Teaching Assistant, KAIST

Jan. 2019  |  Sejong Academy of Science and Arts (SASA; gifted high school), Teaching Assistant, KAIST