Rational design of nanoscale biochemical factories is highly appealing for the next generation of biomanufacturing. Enzymes are the primary building blocks to assemble such molecular factories. My research aims to understand the reaction kinetics and system dynamics of the multienzyme systems, and to integrate the enzymes in a synthetic way to realize defined, programmable and life-like functions in vitro. These efforts will enable new approach to more sophisticated biocatalysis, diagnostics, and adaptive biochemical devices.