Publications

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2024

Ream MW, Randolph LN, Jiang Y, Chang Y, Bao X*, Lian XL*. Direct programming of human pluripotent stem cells into endothelial progenitors with SOX17 and FGF2. Stem Cell Reports (2024). https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(24)00044-4#%20.  

2023

Chang Y, Hummel SN, Jung J, Jin G, Deng Q, Bao X*. Engineered hematopoietic and immune cells derived from human pluripotent stem cells. Exp Hematol 127: 14-27 (2023). https://www.sciencedirect.com/science/article/abs/pii/S0301472X23016740.   

Chang Y, Jin G, Luo W, Luo Q, Jung J, Hummel SN, Torregrosa-Allen S, Elzey BD, Low PS*, Lian X*, Bao X*. Engineered human pluripotent stem cell-derived natural killer cells with PD-L1 responsive immunological memory for enhanced immunotherapeutic efficacy. Bioact Mater 27: 168-180 (2023). https://www.sciencedirect.com/science/article/pii/S2452199X23001123.   

Chang Y, Hummel SN, Watson MN, Jin G, Lian XL, Bao X*. Engineered Artificial Human Neutrophils Exhibit Mature Functional Performance. ACS Synth Biol (2023). https://pubs.acs.org/doi/10.1021/acssynbio.3c00309.  

Repina NA, Johnson HJ, Bao X, Zimmermann JA, Joy DA, Bi SZ, Kane RS, Schaffer DV. Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture. Development 150 (14): dev201386 (2023). https://journals.biologists.com/dev/article/150/14/dev201386/324851/Optogenetic-control-of-Wnt-signaling-models-cell.  

Chang Y, Cai X, Syahirah R, Yao Y, Xu Y, Jin G, Bhute VJ, Torregrosa-Allen S, Elzey BD, Won Y-Y, Deng Q, Lian XL*, Wang X*, Eniola-Adefeso O*, Bao X*. CAR-neutrophil mediated delivery of tumor-microenvironment responsive nanodrugs for glioblastoma chemo-immunotherapy. Nat Commun 14(1): 2266 (2023). https://www.nature.com/articles/s41467-023-37872-4. 

Harris JD, Chang Y, Syahirah R, Lian XL, Deng Q, Bao X*. Engineered anti-prostate cancer CAR-neutrophils from human pluripotent stem cells. J Immunol Regen Med 20: 100074 (2023). https://www.sciencedirect.com/science/article/abs/pii/S2468498823000057. 

Chang Y, Deng Q, Bao X*. A pluripotent road to immunoengineering. Nat Rev Bioeng (2023). https://doi.org/10.1038/s44222-023-00056-2. 

Chang Y, Cai X, Syahirah R, Yao Y, Xu Y, Jin G, Bhute VJ, Torregrosa-Allen S, Elzey BD, Won Y, Deng Q, Lian X*, Wang X*, Eniola-Adefeso O*, Bao X*. CAR-neutrophil mediated delivery of tumor-microenvironment responsive nanodrugs for glioblastoma chemoimmunotherapy. Nature Communications 14, 2266 (2023). https://www.nature.com/articles/s41467-023-37872-4. 

2022

Jung J, Chang Y, Jin G, Deng Q, Lian X, Bao X*. Chemically defined generation of human definitive hematopoietic stem and progenitor cells. STAR Protocols. (2022). https://www.sciencedirect.com/science/article/pii/S2666166722008334. 

Liang P, Chang Y, Jin G, Lian X*, Bao X*. Wnt signaling directs human pluripotent stem cells into vascularized cardiac organoids with chamber-like structures. Front. Bioeng. Biotechnol. (2022). https://www.frontiersin.org/articles/10.3389/fbioe.2022.1059243/full. 

Haideri T, Howells A, Jiang Y, Yang J, Bao X*, Lian X*. Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells. Cell Reports Methods 2(9), 100290 (2022). https://www.sciencedirect.com/science/article/pii/S2667237522001722. 

Chang Y, Syahirah R, Wang X, Jin G, Torregrosa-Allen S, Elzey BD, Hummel SN, Wang T, Lian X*, Deng Q*, Broxmeyer HE, Bao X*. Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy. Cell Reports 40(3), 111128 (2022). https://doi.org/10.1016/j.celrep.2022.111128. 

Jung J, Chang Y, Jin G, Lian X*, Bao X*. Temporal Expression of Transcription Factor ID2 Improves Natural Killer Cell Differentiation from Human Pluripotent Stem Cells. ACS Synth. Biol. doi.org/10.1021/acssynbio.2c00017 (2022). https://pubs.acs.org/doi/full/10.1021/acssynbio.2c00017. 

Chang Y, Syahirah R, Oprescu SN, Wang X, Jung J, Cooper SH, Torregrosa-Allen S, Elzey BD, Hsu AY, Randolph LN, Sun Y, Kuang S, Broxmeyer HE, Deng Q*, Lian X*, Bao X*. Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells. Biomaterials. 285, 121569 (2022).  https://www.sciencedirect.com/science/article/abs/pii/S0142961222002095. 

Chang Y, Syahirah R, Wang X, Jin G, Torregrosa-Allen S, Elzey BD, Hummel SN, Wang T, Lian X*, Deng Q*, Broxmeyer HE, Bao X*. Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy. bioRxiv. doi: https://doi.org/10.1101/2022.03.02.482679 (2022).  https://www.biorxiv.org/content/10.1101/2022.03.02.482679v1.

Rather AM, Xu Y, Chang Y, Dupont RL, Borbora A, Kara UI, Fang J, Mamtani R, Zhang M, Yao Y, Bao X*, Manna U*, Wang X*. Stimuli-Responsive Liquid Crystal-Infused Porous Surfaces for Manipulation of Underwater Gas Bubble Transport and Adhesion. Advanced Materials. e2110085. doi: 10.1002/adma.202110085 (2022). https://onlinelibrary.wiley.com/doi/full/10.1002/adma.202110085.  

Jiang Y, Hoenisch RC, Chang Y, Bao X*, Cameron CE, Lian X*. Robust genome and RNA editing via CRISPR nucleases in PiggyBac systems. Bioactive Materials (2022). https://www.sciencedirect.com/science/article/pii/S2452199X22000615. 

2021 

Xu Y, Chang Y, Yao Y, Zhang M, Dupont RL, Rather AM, Bao X*, Wang X*. Modularizable liquid crystal-based open surfaces enable programmable chemical transport and feeding using liquid droplets. Adv. Mater. Accepted (2021).  

Hellwarth PB, Chang Y, Das A, Liang P, Lian X*, Repina NA*, Bao X*. Optogenetic-mediated cardiovascular differentiation and patterning of human pluripotent stem cells. Adv. Genet (2021). https://onlinelibrary.wiley.com/doi/10.1002/ggn2.202100011.   

Jiang Y, Chen C, Randolph LN, Ye S, Zhang X, Bao X, Lian X. Generation of pancreatic progenitors from human pluripotent stem cells by small molecules.  Stem Cell Reports 16(9):2395-2409 (2021). https://www.cell.com/stem-cell-reports/fulltext/S2213-6711(21)00387-8.

Randolph LN, Jiang Y, Chang Y, Bao X*, Lian X*. Direct induction of hemogenic endothelial progenitors from hPSCs by defined factors revealed by single-cell transcriptome analysis. BioRxiv. 435636 (2021). www.biorxiv.org/content/10.1101/2021.03.18.435636v3.abstract  

Jiang Y, Bao X*, Lian XL*. A dual cardiomyocyte reporter model derived from human pluripotent stem cells. Stem Cell Res Ther 12 (1), 305 (2021). pubmed.ncbi.nlm.nih.gov/34051863/ 

Xu Y, Rather AM, Song S, Fang J, Dupont RL, Kara UI, Chang Y, Paulson JA*, Qin R*, Bao X*, Wang X*. Ultrasensitive and Selective Detection of SARS-CoV-2 Using Thermotropic Liquid Crystals and Image-Based Machine Learning Cell Rep Phys Sci. 1 (12): 100276 (2021). doi: 10.1016/j.xcrp.2020.100276. pubmed.ncbi.nlm.nih.gov/33225318/ 

2020

Chang Y, Bao X*. Adoptive natural killer cell therapy: a human pluripotent stem cell perspective. Curr Opin Chem Eng 30, 69-76 (2020). www.sciencedirect.com/science/article/abs/pii/S221133982030054X 

Muckom R, Bao X, Tran E, Chen E, Murugappan A, Dordick JS, Clark DS, Schaffer DV. High-throughput 3D screening for differentiation of hPSC-derived cell therapy candidates. Sci. Adv. 6 (32), eaaz1457 (2020).  pubmed.ncbi.nlm.nih.gov/32821815/       

Repina NA, McClave T, Johnson HJ, Bao X, Kane RS, Schaffer DV. Engineered illumination devices for optogenetic control of cellular signaling dynamics. Cell Rep, 107737 (2020).  pubmed.ncbi.nlm.nih.gov/32521262/ 

Chang Y, Hellwarth PB, Randolph LN, Sun Y, Xing Y, Zhu W, Lian XL*, Bao X*. Fluorescent Indicators for Continuous and Lineage-Specific Reporting of Cell Cycle Phases in Human Pluripotent Stem Cells. Biotechnol Bioeng,  117 (7): 2177-2186 (2020). pubmed.ncbi.nlm.nih.gov/32277708/ 

2019

Xu Y, Chen C, Hellwarth PB, Bao X*. Biomaterials for stem cell engineering and biomanufacturing. Bioact Mater 4, 366-379 (2019). pubmed.ncbi.nlm.nih.gov/31872161/

Bao X, Adil MM,  Muckom R, Zimmermann JA, Tran A, Suhy N, Xu Y, Sampayo RG, Clark DS, Schaffer DV. Gene editing to generate versatile human pluripotent stem cell reporter lines for analysis of differentiation and lineage tracing. Stem Cells 37 (12), 1556-66 (2019). pubmed.ncbi.nlm.nih.gov/31634414/ 

Repina NA, Bao X,  Zimmermann JA, Joy DA, Kane RS, Schaffer DV. Optogenetic control of Wnt signaling for modeling early embryonic patterning with human pluripotent stem cells. BioRxiv. 665695 (2019). www.biorxiv.org/content/10.1101/665695v1.full 

Jiang Y, Zhou Y, Bao X, Chen C, Randolph LN, Du J, Lian XL. An ultrasensitive calcium reporter system via CRISPR/Cas9-mediated genome editing in human pluripotent stem cells. iScience. 9, 27-35 (2019).  pubmed.ncbi.nlm.nih.gov/30368079/ 

Randolph LN, Bao X, Oddo M, Lian XL. Sex-dependent VEGF expression underlies variations in human pluripotent stem cell to endothelial progenitor differentiation. Sci. Rep. 9 (1): 16696 (2019). pubmed.ncbi.nlm.nih.gov/31723192/ 

Qian T, Hernday SE, Bao X, Olson WR, Panzer SE, Shusta EV, Palecek SP. Directed differentiation of human pluripotent stem cells to podocytes under defined conditions. Sci. Rep. 9: 2765 (2019). pubmed.ncbi.nlm.nih.gov/30808965/ 

Prior to Purdue

Qian T, Maguire SE, Canfield SG, Bao X, Olson WR, Shusta EV, Palecek SP. Directed differentiation of human pluripotent stem cells to blood-brain barrier endothelial cells. Sci. Adv. 3 (11), e1701679 (2017). pubmed.ncbi.nlm.nih.gov/29134197/  

Bao X, Lian X, Qian T, Bhute VJ, Han T, Palecek SP. Directed differentiation of self-renewing epicardial cells from human pluripotent stem cells by modulating canonical Wnt signaling. Nat. Protoc. 12, 1890-1900 (2017). doi:10.1038/nprot.2017.080. pubmed.ncbi.nlm.nih.gov/28817124/ 

Bhute VJ#, Bao X#, Dunn KK, Knutson KR, McCurry EC, Jin G, Lee W, Lewis S, Ikeda A, Palecek SP. Metabolomics identifies metabolic markers of maturation in human pluripotent stem cell-derived cardiomyocytes. Theranostics 7(7), 2078-91 (2017). doi:10.7150/thno.19390. pubmed.ncbi.nlm.nih.gov/28656061/  

Bao X#, Bhute VJ#, Han T, Lian X, Palecek SP. hPSC-derived epicardial progenitors can differentiate into endocardial-like endothelial cells. Bioeng. Transl. Med. 2(2): 191-201 (2017). doi: 10.1002/btm2.10062. pubmed.ncbi.nlm.nih.gov/29170757/ 

Randolph LN#, Bao X#, Zhou C, Lian X. An all-in-one, Tet-on 3G inducible PiggyBac system for human pluripotent stem cells and derivatives. Sci. Rep. 7, 1549 (2017). doi:10.1038/s41598-017-01684-6. pubmed.ncbi.nlm.nih.gov/28484230/ 

Moon J, Zhou H, Zhang L, Tan W, Liu Y, Zhang S, Morlock L, Bao X, Palecek SP, Feng J, Williams NS, Amatruda JF, Olson EN, Bassel-Duby R, Lum L. Blockade to pathological remodeling of infarcted heart tissue using a porcupine antagonist. Proc. Natl. Acad. Sci. U.S.A. 114, 1649-54 (2017). pubmed.ncbi.nlm.nih.gov/28143939/ 

Bhute VJ, Bao X, Palecek SP. Advances in applications of metabolomics in pluripotent stem cell research. Curr. Opin. Chem. Eng. 15, 36-43 (2017). doi:10.1016/j.coche.2016.11.007. pubmed.ncbi.nlm.nih.gov/28729963/ 

Bao X, Lian X, Hacker TA, Schmuck EG, Qian T, Bhute VJ, Han T, Shi M, Drowley L, Plowright A, Wang Q, Goumans M, Palecek SP. Long-term self-renewing human epicardial cells generated from pluripotent stem cells under xeno-free conditions. Nat. Biomed Eng. 1, 003 (2016). doi:10.1038/s41551-016-0003. pubmed.ncbi.nlm.nih.gov/28462012/  

Bao X, Lian X, Palecek SP. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions Methods Mol Biol. 1481: 183-96 (2016). doi: 10.1007/978-1-4939-6393-5_17. pubmed.ncbi.nlm.nih.gov/27590162/ 

Bhute VJ, Ma Y, Bao X, Palecek SP. The poly-(ADR-Ribose) polymerase inhibitor Veliparib and Radiation cause significant cell line dependent metabolic changes in breast cancer cells. Sci. Rep. 6, 36061 (2016). doi:  10.1038/srep36061. pubmed.ncbi.nlm.nih.gov/27811964/  

Cossette SM, Bhute VJ, Bao X, Harmann LM, Horswill MA, Sinha I, Gastonguay A, Pooya S, Bordas M, Kumar SN, Mirza SP, Palecek SP., Strande JL, Ramchandran R. Sucrose non-fermenting related kinase enzyme mediated Rho-associated kinase signaling is responsible for cardiac function. Circ. Cardiovasc. Genet. 6, 474-486 (2016). doi: 10.1161/CIRCGENETICS.116.001515. pubmed.ncbi.nlm.nih.gov/27780848/ 

Lian X, Xu J, Bao X, Randolph LN. Interrogating canonical Wnt signaling pathway in human pluripotent stem cell fate decisions using CRISPR-Cas9. Cell Mol. Bioeng. 9, 325-334 (2016). doi:10.1007/s12195-016-0453-8. link.springer.com/article/10.1007/s12195-016-0453-8

Lian X#, Bao X#, Zilberter M, Westman M, Fisahn A, Hsiao C, Hazeltine LB, Dunn KK, Kamp TJ, Palecek SP. Chemically defined, albumin-free human cardiomyocyte generation. Nat. Methods 12, 595-596 (2015). doi:10.1038/nmeth.3448. pubmed.ncbi.nlm.nih.gov/26125590/ 

Bao X#, Lian X#, Dunn KK, Shi M, Han T, Qian T, Bhute VJ, Canfield SG, Palecek SP. Chemically defined albumin-free differentiation of human pluripotent stem cells to endothelial progenitor cells. Stem Cell Res 15, 122-129 (2015). doi:10.1016/j.scr.2015.05.004 (F1000Prime).  pubmed.ncbi.nlm.nih.gov/26042795/  

Lian X#, Bao X#, Al-Ahmad A, Liu J, Wu Y, Dong W, Dunn KK, Shusta EV, Palecek SP. Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small molecule activation of WNT signaling. Stem Cell Reports 3, 804-816 (2014). doi:10.1016/j.stemcr.2014.09.005. pubmed.ncbi.nlm.nih.gov/25418725/ 

Cossette SM, Gastonguay AJ, Bao X, Lerch-Gaggl A, Zhong L, Harmann LM, Chun C, Li K, Foeckler J, Bordas M, Weiler H, Strande JL, Palecek SP, Ramchandran R. Sucrose non-fermenting related kinase enzyme is essential for cardiac metabolism. Biology Open 4, 48-61 (2014). doi: 10.1242/bio.20149811. pubmed.ncbi.nlm.nih.gov/25505152/ 

Lian X, Selekman J, Bao X, Hsiao C, Zhu K, Palecek SP. A small molecule inhibitor of SRC family kinases promotes simple epithelial differentiation of human pluripotent stem cells. PLoS One 8, e60016 (2013). doi: 10.1371/journal.pone.0060016. pubmed.ncbi.nlm.nih.gov/23527294/ 

Lian X, Zhang J, Azarin SM, Zhu K, Hazeltine LB, Bao X, Hsiao C, Kamp TJ, Palecek SP. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nat. Protoc. 8, 162-75 (2013). doi:10.1038/nprot.2012.150.  pubmed.ncbi.nlm.nih.gov/23257984/ 

Xu D, Tonggu L, Bao X, Lu D, Liu Z. Activation and stabilization of a lipase nanogel using GMA for acryloylation. Soft Matter 6, 2036-2042 (2012). doi: 10.1039/C1SM06853A. pubs.rsc.org/en/content/articlelanding/2012/sm/c1sm06853a#!divAbstract