Research

Our research aims to elucidate how neural circuits are established in the developing cortex, how they are shaped by sensory experience, and how these processes are disrupted in disease states. We investigate the spatiotemporal mechanisms underlying activity-dependent synapse formation, plasticity, and elimination by combining two-photon microscopy with electrophysiology.

Using two-color, two-photon neurotransmitter uncaging (e.g., glutamate, GABA, serotonin, dopamine, and nicotine etc), we precisely stimulate individual synapses and neurons with distinct neurotransmitters to closely mimic natural patterns of neural activity in live animals and brain slices. In addition, we integrate in vivo pharmacology, chemogenetics, optogenetics, and calcium imaging with two-photon imaging to manipulate and monitor neuronal activity in real time. Together, this multidisciplinary approach allows us to define how single synapses and neurons contribute to the refinement of functional neural circuits.

On-going projects

• Neuromodulatory Control of Synaptic Development 

• Excitatory-Inhibitory (E/I) Synaptic Crosstalk 

• Neurodevelopmental Disorders (Autism, Down Syndrome, & Early Life Stress) 

• Developmental Impact of Prenatal/Postnatal/Adolescent Drug Exposures (e.g. Nicotine, CBD, Fentanyl, Psilocybin)