professional skills

☞ Cellular and Molecular Biology

·       2D mammalian cell culture (hepatocytes, hepatic stellate cells, Kupffer cells, adipocytes, skeletal muscle cells, melanocytes, immune cells)

·       RNA and protein extraction, quantitative PCR (qPCR), Western blotting, immunofluorescence (IF), ELISA

·       Gene perturbation techniques: gene inhibitor

·       Reporter assays (luciferase activity, pathway analysis)

·       Cell viability, apoptosis, oxidative stress, and mitochondrial function assays

·       Immune cell types identification using a flow cytometer (FACS)

·       Isolation of mouse peritoneal monocytes and splenocytes.

☞ Animal Models

·       Murine models of metabolic liver disease: NAFLD/MAFLD, obesity, insulin resistance, type 2 diabetes

·       High-fat/high-fructose diet feeding models, fasting, and refeeding murine model

·       Chemically induced liver injury and fibrosis (CCl₄, TAA, DMN, alcohol, bile duct ligation)

·       Functional gastrointestinal disease animal models (loperamide-induced dysmotility, cold stress, PPI-induced dyspepsia)

·       Osteoporosis and sarcopenia mouse models (ovariectomy, aging-related bone loss)

·       In vivo drug efficacy testing, metabolic phenotyping (body weight, lipid/glucose tolerance, insulin sensitivity, energy metabolism)

☞ Microbiome and Omics

·       Gut microbiota profiling (16S rRNA sequencing, metagenomics analysis)

·       Multi-omics integration: transcriptomics, proteomics, metabolomics, bile acid-targeted metabolomics

·       Fecal microbiota transplantation (FMT) in obesity and NAFLD models

·       Microbiome-metabolite interaction studies (HPLC/ELISA: short-chain fatty acids, tryptophan derivatives, bile acids)

☞ Biochemical & Pathophysiological Assays

·       Lipid accumulation and lipogenesis/β-oxidation assays

·       Glucose and insulin tolerance tests (OGTT, ITT)

·       Inflammatory cytokine measurement (ELISA: IL-6, TNF-α, MCP-1, etc.)

·       Antioxidant activity, total antioxidant capacity (TAC) assays

·       Histopathological analyses (H&E, Oil Red O, Sirius Red, IHC, IF for fibrosis and inflammation markers)

·       Serum/liver enzyme assays (ALT, AST, ALP, TG, TC, HDL, LDL)

☞ Research & Team Leadership

·       Design and optimization of in vitro, ex vivo, and in vivo experiments aligned with translational applications

·       Grant writing (NRF Korea, PI on multi-year funded projects)

·       Patent acquisition (2 registered Korean patents)

·       Editorial & Perspective (8 publications as Editor)

·       Manuscript preparation and publication in SCIE journals (>70 publications, including first/corresponding author 31 publications, h-index 30, >2,500 citations)

·       Mentorship and supervision of undergraduate, graduate students, postdocs, and researchers