Sample images of Stained Liver tissue sections are given in the table below. One can select the image of the plant tissue section to be analyzed from the provided samples and follow the steps in the Procedure to complete the experiment.

Right click on any of the following image 1,image 2, image 3 or image 4 and follow "Save image as" to save the image on the computer and then analyze the image.

Step 2: To analyze an Image into ImageJ, we have to import the image into ImageJ. To perform that, Go to files ---> Open. A popup window will appear. Use Choose button to choose your respective images into ImageJ software. The imported picture will shown in a new window

Step 3: Convert the images into "grayscale” (go to image ---> Type ---> RGB stack).

Step 4: Adjust the RGB grey scale manually by moving the lower slider

Step 5: Use the Image ---> Stacks ---> Make Montage command to view all three channels at the same time.

Step 6: Adjust the Image ---> Adjust ---> Threshold tool. The threshold must be manually adjusted by moving the lower slider until the stained liver tissue is highlighted in red.

Step 7: Set threshold level and press “OK”

Step 8: Choose AnalyzeSet Measurements. The Set Measurements window opens. Select Area, Area fraction, Limit to Threshold and Display label measurements. Then click OK.

Step 9: To measure the selected area. Press Analyze ---> Measure. The Area and mean area of the stained samples appears in a new window. Repeat the above steps until all bands have been measured. Then copy all the values to a spreadsheet. The quantified images are now ready for data analysis in the spreadsheet program.

Result Interpretation:

“Results” window indicates the Area and Percentage area of the stained cells. Here 23.2555% of the area contains the stained cells.