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This in vivo, prospective, randomized, single-blinded study histologically compared biofilm/necrotic debridement efficiency of a hand/rotary technique versus a hand/rotary/1 min ultrasound technique in the mesial roots of necrotic, human mandibular molars. The hand/rotary group consisted of 20 mesial roots. The hand/rotary/ultrasound group consisted of 20 mesial roots prepared with the same hand/rotary technique followed by 1 min of ultrasonic irrigation, per canal, utilizing an ultrasonic needle in a MiniEndo unit. Following extraction, histologic preparation and staining, 0.2 mum cross-sections from the 1- to 3-mm apical levels were evaluated for percentage of biofilm/necrotic debris removal. Cleanliness results at the 1-, 2- and 3-mm levels for the hand/rotary and hand/rotary/ultrasound techniques, respectively, were: Canals, 80% versus 95%, 92% versus 99%, and 95% versus 100%; Isthmuses, 33% versus 83%, 31% versus 86%, 45% versus 91%. Statistical analysis revealed mean percent canal and isthmus cleanliness values to be significantly higher for hand/rotary/ultrasound technique at all levels evaluated.
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Earlier it was Error waiting for a debug connection: Bad state: No element, I switched to master from stable and now it is throwing this error on run. App is getting installed but crashing by throwing this error.
You need to execute flutter clean command in your project and also make sure the device properly connected, the cable is attached properly. Also, you have to allow permission to launch on an app device and you should see a dialog while launching the app and restart the device...
I was facing the same issue, the problem occurred after I tried to change the package name of my flutter (Android App) may be from the old article. After reverting back the changes i.e setting default"com.example.app" as the package name issue disappears. If someone else is facing the issue, you can follow these two articles 1 or 2. In the first link, you might be having Points 1 & 2 a bit different you may not have any of these two:
Responding to "Error waiting for a debug connection: The log reader stopped unexpectedly" ... In the end I found that my code was not the cause of this error. I found that the error is confirming a communication issue between the Flutter Console and the current emulator. I found that when I switched between emulators, that another emulator didn't have the same error or issue and everything worked fine. So, my conclusion is that the error presents itself where there is a software flaw regarding the current emulator, which should mean that a software update or an alternative emulator is the way around the problem.
I have had the same problem for a whole week after upgrading to flutter 1.17....i did flutter clean and even downgraded to flutter 1.12 non of them worked...but when i upgraded my sdk tools(specifically flutter)the problem disappeared.
Once I finally got this reproduced, looking at the journal I noticed flutter was crashing in the swrast GL driver. The issue here is that flutter is using GL drivers from within the snap rather than the host.
We had forced this in the past to work around VS Code setting LIBGL_DRIVERS_PATH to a path within its own snap. Looks like we should not have redirected that to the Flutter snap but rather to the host.
If you are on Mac, this might be because you don't have sudo access. I think the debugger needs to read iOS logs which requires sudo access. I was able to fix the issue by adding admin rights to my account (system preferences > User&Groups > Allow user to administer this computer). Of course, this might not be possible for everyone.
I have also encountered this error and I resolve this by going to myPhone's Settings and then in"Developer Option",there was an option "Select debug app"then choose your app from the list (if you are running multiple projects else there will be 1 app).Now your app will get debug access.
If this happens while launching a linux desktop build from the vscode debugger then it could be because you have both vscode and flutter installed as snaps. To solve, remove the vscode snap snap remove code and re-install it using another method such as downloading the .tar.gz and extracting it to a suitable directory. Your vscode settings and extensions will be preserved when you remove the snap.
Even I faced a similar problem while debugging the app and then I disabled the USB debugging (security settings) and Revoke USB debugging authorizations in the developer option
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Here, we report the development of a microplate reader-based system for visualizing gene expression dynamics in living bacterial cells in response to a fungus in space and real-time. A bacterium expressing the red fluorescent protein mCherry fused to the promoter region of a regulator gene nunF indicating activation of an antifungal secondary metabolite gene cluster was used as a reporter system. Time-lapse image recordings of the reporter red signal and a green signal from fluorescent metabolites combined with microbial growth measurements showed that nunF-regulated gene transcription is switched on when the bacterium enters the deceleration growth phase and upon physical encounter with fungal hyphae. This novel technique enables real-time live imaging of samples by time-series multi-channel automatic recordings using a microplate reader as both an incubator and image recorder of general use to researchers. The technique can aid in deciding when to destructively sample for other methods e.g. transcriptomics and mass spectrometry imaging to study gene expression and metabolites exchanged during the interaction.
Microbial interactions are found in numerous biological processes ranging from mixed infections in humans, animals and plants, to the development of fermented foods or the treatment of waste water. Thus, understanding how microbes coexist and interact in such communities is essential for fighting polymicrobial infections and for the development of robust, mixed microbial cultures for applications within biotechnology. In order to achieve this, a technology toolbox is essential. Current technologies available for studying microbial interactions include gene expression techniques (qPCR1, microarrays2, 3, and RNA sequencing4, 5), the identification of secondary metabolites by imaging mass spectrometry (IMS)6,7,8 methods and tools for gene deletion, replacement or expression control such as CRISPR-Cas9 technology9. Previously, we demonstrated that a combination of genomics, molecular genetics and microbiology coupled with IMS analysis could be used to unravel the mode of action underlying the antagonism of Pseudomonas fluorescens In5 against diverse phytopathogens10. More recently, we have also used transcriptomics to study gene expression in this biocontrol bacterium during phytopathogen interactions (manuscript in preparation). However, a disadvantage of transcriptomics and IMS analysis is the destructive nature of the technologies which are difficult to adapt for in vivo measurements to provide space and time information. Furthermore, current technologies can be time-consuming and expensive requiring sampling at numerous specific time points in order to study the dynamics of an interaction between microorganisms. Therefore, to investigate in more detail the interaction of our model organism P. fluorescens strain In5 with a phytopathogen, we have adapted a standard microplate reader as a tool for image-based real-time gene expression analysis of living cells.
P. fluorescens strain In5 has a high potential for secondary metabolite production and recently, the two non-ribosomal peptides (NRPs) nunamycin and nunapeptin have been shown to underpin the antimicrobial activity of this isolate against diverse phytopathogens including Fusarium graminearum 10, 11. Functional characterization of this strain during interactions with pathogens relies on assays where the bacterium is grown in co-culture with a pathogen on solid media. Many secondary metabolite gene clusters are silent under standard laboratory conditions and in particular fungi often produce increased amounts of secondary metabolites when cultivated on agar-based surfaces compared to in liquid media12. We have observed that culture conditions also play an important role in secondary metabolite production for strain In5. To date, only nunapeptin production has been detected in liquid media, whereas both peptides are produced on agar-based media. In addition to nunamycin and nunapeptin, strain In5 also produces a green fluorescent compound, most likely the iron chelating siderophore pyoverdine, which is known to be synthesized by several pseudomonads13. Thus a key challenge in the analysis of such interactions is the non-destructive measurement of target gene expression in space and in real-time using living cells in co-culture on solid surfaces.
Reporter strains that conditionally express fluorescent proteins (e.g. mCherry or GFP) are useful tools for gene expression profiling in living cells. Such strains can be monitored in microtiter plates grown in liquid medium and used to indicate for example the production of a compound whereby increased fluorescence from the fluorophore fused to a selected promoter or gene will signal the upregulation of biosynthetic genes. The fluorescent protein signal dynamics can then be measured in relation to biomass growth as an indicator of secondary metabolite production per cell over time. 152ee80cbc
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