Virus Glycoprotein Trafficking Lab
Established. 2019@Center for Biomolecular Therapeutics
University of Maryland School of Medicine, Rockville MD
Established. 2019@Center for Biomolecular Therapeutics
University of Maryland School of Medicine, Rockville MD
Publications
Dey, D, Qing E, He Y, Chen Y, Jennings B, Cohn W, Singh S, Gakhar L, Schnicker NJ, Pierce BG, Whitelegge JP, Doray B, Orban JP, Gallagher T, Hasan, SS. A single C-terminal residue controls SARS-CoV-2 spike trafficking and VLP assembly. Nature Communications (2023), Pubmed PMID: 38102143
Mishra AK, Shahid S, Karade SS, Agnihotri P, Kolesnikov A, Hasan SS, Mariuzza RA. CryoEM structure of a therapeutic antibody (favezelimab) bound to human LAG3 determined using a bivalent Fab as fiducial marker, Structure. (accepted 2023), Pubmed PMID: 37619561
Dey, D, Hasan, SS. Strategies for rapid production of crystallization quality coatomer WD40 domains, Protein Expr Purif. (accepted, 2023), Pubmed PMID: 37625737
Dey D, Singh S, Khan S, Martin M, Schnicker NJ, Gakhar L, Pierce BG, Hasan SS. An extended motif in the SARS-CoV-2 spike modulates binding and release of host coatomer in retrograde trafficking. Commun Biol. 2022 Feb 8;5(1):115. doi: 10.1038/s42003-022-03063-y. PubMed PMID: 35136165; PubMed Central PMCID: PMC8825798.
Hasan SS, Dey D, Singh S, Martin M. The structural biology of eastern equine encephalitis virus, an emerging viral threat. Pathogens. 2021 Jul 31;10(8). doi: 10.3390/pathogens10080973. Review. PubMed PMID: 34451437; PubMed Central PMCID: PMC8400090.
Dey D, Poudyal S, Rehman A, Hasan SS. Structural and biochemical insights into flavivirus proteins. Virus Res. 2021 Apr 15;296:198343. doi: 10.1016/j.virusres.2021.198343. Epub 2021 Feb 16. Review. PubMed PMID: 33607183.
Before Joining UMB:
Chen CL, Hasan SS, Klose T, Sun Y, Buda G, Sun C, Klimstra WB, Rossmann MG. Cryo-EM structure of eastern equine encephalitis virus in complex with heparan sulfate analogues. Proc Natl Acad Sci U S A. 2020 Apr 21;117(16):8890-8899. doi: 10.1073/pnas.1910670117. Epub 2020 Apr 3. PubMed PMID: 32245806; PubMed Central PMCID: PMC7183182.select
Parvate AD, Vago F, Hasan SS, Lee J, Williams EP, Lanman J, Jonsson CB. A new inactivation method to facilitate cryo-EM of enveloped, RNA viruses requiring high containment: A case study using Venezuelan Equine Encephalitis Virus (VEEV). J Virol Methods. 2020 Mar;277:113792. doi: 10.1016/j.jviromet.2019.113792. Epub 2019 Nov 28. PubMed PMID: 31786314.ect
Hasan SS, Sun C, Kim AS, Watanabe Y, Chen CL, Klose T, Buda G, Crispin M, Diamond MS, Klimstra WB, Rossmann MG. Cryo-EM Structures of eastern equine encephalitis virus reveal mechanisms of virus disassembly and antibody neutralization. Cell Rep. 2018 Dec 11;25(11):3136-3147.e5. doi: 10.1016/j.celrep.2018.11.067. PubMed PMID: 30540945; PubMed Central PMCID: PMC6302666.
Hasan SS, Sevvana M, Kuhn RJ, Rossmann MG. Structural biology of zika virus and other flaviviruses. Nat Struct Mol Biol. 2018 Jan;25(1):13-20. doi: 10.1038/s41594-017-0010-8. Epub 2018 Jan 8. Review. PubMed PMID: 29323278.
Yap ML, Klose T, Urakami A, Hasan SS, Akahata W, Rossmann MG. Structural studies of chikungunya virus maturation. Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):13703-13707. doi: 10.1073/pnas.1713166114. Epub 2017 Dec 4. PubMed PMID: 29203665; PubMed Central PMCID: PMC5748190.
Hasan SS, Miller A, Sapparapu G, Fernandez E, Klose T, Long F, Fokine A, Porta JC, Jiang W, Diamond MS, Crowe JE Jr, Kuhn RJ, Rossmann MG. A human antibody against zika virus crosslinks the E protein to prevent infection. Nat Commun. 2017 Mar 16;8:14722. doi: 10.1038/ncomms14722. PubMed PMID: 28300075; PubMed Central PMCID: PMC5356071.
Bhaduri S, Stadnytskyi V, Zakharov SD, Hasan SS, Bujnowicz Ł, Sarewicz M, Savikhin S, Osyczka A, Cramer WA. Pathways of transmembrane electron transfer in cytochrome bc complexes: Dielectric heterogeneity and interheme coulombic interactions. J Phys Chem B. 2017 Feb 9;121(5):975-983. doi: 10.1021/acs.jpcb.6b11709. Epub 2017 Jan 25. PubMed PMID: 28032998.
Singh SK, Hasan SS, Zakharov SD, Naurin S, Cohn W, Ma J, Whitelegge JP, Cramer WA. Trans-membrane signaling in photosynthetic state transitions: Redox- and structure-dependent interaction in vitro between stt7 kinase and the cytochrome b6f complex. J Biol Chem. 2016 Oct 7;291(41):21740-21750. doi: 10.1074/jbc.M116.732545. Epub 2016 Aug 18. PubMed PMID: 27539852; PubMed Central PMCID: PMC5076842.
Hasan SS, Cramer WA. Structure-function of the cytochrome b6f lipoprotein complex. In: Cramer WA, Kallas T, editors. Cytochrome Complexes: Evolution, Structures, Energy Transduction, and Signaling Switzerland: Springer; 2016. p.177-207.
Baniulis D, Hasan SS, Miliute I, Cramer WA. Mechanisms of superoxide generation and signaling in cytochrome bc complexes. In: Cramer WA, Kallas T, editors. Cytochrome Complexes: Evolution, Structures, Energy Transduction, and Signaling Switzerland: Springer; 2016. p.397-417.
Agarwal R, Hasan SS, Jones LM, Stofleth JT, Ryan CM, Whitelegge JP, Kehoe DM, Cramer WA. Role of domain swapping in the hetero-oligomeric cytochrome b6f lipoprotein complex. Biochemistry. 2015 May 26;54(20):3151-63. doi: 10.1021/acs.biochem.5b00279. Epub 2015 May 12. PubMed PMID: 25928281; PubMed Central PMCID: PMC4672631.
Agarwal R, Zakharov S, Hasan SS, Ryan CM, Whitelegge JP, Cramer WA. Structure-function of cyanobacterial outer-membrane protein, Slr1270: homolog of Escherichia coli drug export/colicin import protein, TolC. FEBS Lett. 2014 Nov 3;588(21):3793-801. doi: 10.1016/j.febslet.2014.08.028. Epub 2014 Sep 13. PubMed PMID: 25218435; PubMed Central PMCID: PMC4288923.
Hasan SS, Proctor EA, Yamashita E, Dokholyan NV, Cramer WA. Traffic within the cytochrome b6f lipoprotein complex: gating of the quinone portal. Biophys J. 2014 Oct 7;107(7):1620-8. doi: 10.1016/j.bpj.2014.08.003. PubMed PMID: 25296314; PubMed Central PMCID: PMC4190601.
Hasan SS, Cramer WA. Internal lipid architecture of the hetero-oligomeric cytochrome b6f complex. Structure. 2014 Jul 8;22(7):1008-15. doi: 10.1016/j.str.2014.05.004. Epub 2014 Jun 12. PubMed PMID: 24931468; PubMed Central PMCID: PMC4105968.
Hasan SS, Zakharov SD, Chauvet A, Stadnytskyi V, Savikhin S, Cramer WA. A map of dielectric heterogeneity in a membrane protein: the hetero-oligomeric cytochrome b6f complex. J Phys Chem B. 2014 Jun 19;118(24):6614-25. doi: 10.1021/jp501165k. Epub 2014 Jun 11. PubMed PMID: 24867491; PubMed Central PMCID: PMC4067154.
Singh NK, Hasan SS, Kumar J, Raj I, Pathan AA, Parmar A, Shakil S, Gourinath S, Madamwar D. Crystal structure and interaction of phycocyanin with β-secretase: A putative therapy for Alzheimer's disease. CNS Neurol Disord Drug Targets. 2014;13(4):691-8. doi: 10.2174/1871527313666140228114456. PubMed PMID: 24576002.
Baniulis D, Hasan SS, Stofleth JT, Cramer WA. Mechanism of enhanced superoxide production in the cytochrome b(6)f complex of oxygenic photosynthesis. Biochemistry. 2013 Dec 17;52(50):8975-83. doi: 10.1021/bi4013534. Epub 2013 Dec 6. PubMed PMID: 24298890; PubMed Central PMCID: PMC4037229.
Hasan SS, Baniulis D, Yamashita E, Zhalnina MV, Zakharov SD, Stofleth JT, Cramer WA. Methods for studying interactions of detergents and lipids with α-helical and β-barrel integral membrane proteins. Curr Protoc Protein Sci. 2013 Nov 5;74:29.7.1-29.7.30. doi: 10.1002/0471140864.ps2907s74. PubMed PMID: 24510648; PubMed Central PMCID: PMC4062877.
Hasan SS, Yamashita E, Cramer WA. Transmembrane signaling and assembly of the cytochrome b6f-lipidic charge transfer complex. Biochim Biophys Acta. 2013 Nov-Dec;1827(11-12):1295-308. doi: 10.1016/j.bbabio.2013.03.002. Epub 2013 Mar 16. Review. PubMed PMID: 23507619; PubMed Central PMCID: PMC4029431.
Rizvi A, Hasan SS, Naseem I. Selective cytotoxic action and DNA damage by calcitriol-Cu(II) interaction: Putative mechanism of cancer prevention. PLoS One. 2013;8(9):e76191. doi: 10.1371/journal.pone.0076191. eCollection 2013. PubMed PMID: 24086705; PubMed Central PMCID: PMC3785422.
Hasan SS, Rizvi A, Naseem I. Calcitriol-induced DNA damage: Toward a molecular mechanism of selective cell death. IUBMB Life. 2013 Sep;65(9):787-92. doi: 10.1002/iub.1199. Epub 2013 Aug 5. PubMed PMID: 23913678.
Hasan SS, Stofleth JT, Yamashita E, Cramer WA. Lipid-induced conformational changes within the cytochrome b6f complex of oxygenic photosynthesis. Biochemistry. 2013 Apr 16;52(15):2649-54. doi: 10.1021/bi301638h. Epub 2013 Apr 5. PubMed PMID: 23514009; PubMed Central PMCID: PMC4034689.
Hasan SS, Yamashita E, Baniulis D, Cramer WA. Quinone-dependent proton transfer pathways in the photosynthetic cytochrome b6f complex. Proc Natl Acad Sci U S A. 2013 Mar 12;110(11):4297-302. doi: 10.1073/pnas.1222248110. Epub 2013 Feb 25. PubMed PMID: 23440205; PubMed Central PMCID: PMC3600468.
Cramer WA, Yamashita E, Baniulis D, Whitelegge JP, Hasan SS. Structure-function of the cytochrome b6f complex of oxygenic photosynthesis. In: Lennarz WJ, Lane MD, editors. Encyclopedia of Biological Chemistry USA: Elsevier; 2013. p.161-171.
Cramer WA, Yamashita E, Hasan SS. The cytochrome b6f complex of oxygenic photosynthesis. In: Roberts GK, editor. Encyclopedia of Biophysics USA: Springer; 2013. p.417-422.
Hasan SS, Cramer WA. Lipid functions in cytochrome bc complexes: An odd evolutionary transition in a membrane protein structure. Philos Trans R Soc Lond B Biol Sci. 2012 Dec 19;367(1608):3406-11. doi: 10.1098/rstb.2012.0058. PubMed PMID: 23148267; PubMed Central PMCID: PMC3497066.
Hasan SS, Cramer WA. On rate limitations of electron transfer in the photosynthetic cytochrome b6f complex. Phys Chem Chem Phys. 2012 Oct 28;14(40):13853-60. doi: 10.1039/c2cp41386h. Epub 2012 Aug 14. PubMed PMID: 22890107; PubMed Central PMCID: PMC3505453.
Cramer WA, Zakharov SD, Hasan SS, Zhang H, Baniulis D, Zhalnina MV, Soriano GM, Sharma O, Rochet JC, Ryan C, Whitelegge J, Kurisu G, Yamashita E. Membrane proteins in four acts: Function precedes structure determination. Methods. 2011 Dec;55(4):415-20. doi: 10.1016/j.ymeth.2011.11.001. Epub 2011 Nov 10. PubMed PMID: 22079407; PubMed Central PMCID: PMC3282554.
Hasan SS, Yamashita E, Ryan CM, Whitelegge JP, Cramer WA. Conservation of lipid functions in cytochrome bc complexes. J Mol Biol. 2011 Nov 18;414(1):145-62. doi: 10.1016/j.jmb.2011.09.023. Epub 2011 Sep 28. PubMed PMID: 21978667; PubMed Central PMCID: PMC3215850.
Cramer WA, Hasan SS, Yamashita E. The Q cycle of cytochrome bc complexes: A structure perspective. Biochim Biophys Acta. 2011 Jul;1807(7):788-802. doi: 10.1016/j.bbabio.2011.02.006. Epub 2011 Feb 23. Review. PubMed PMID: 21352799; PubMed Central PMCID: PMC3101715.
Baniulis D, Zhang H, Zakharova T, Hasan SS, Cramer WA. Purification and crystallization of the cyanobacterial cytochrome b6f complex. Methods Mol Biol. 2011;684:65-77. doi: 10.1007/978-1-60761-925-3_7. PubMed PMID: 20960122.
Ryan CM, Souda P, Bassilian S, Ujwal R, Zhang J, Abramson J, Ping P, Durazo A, Bowie JU, Hasan SS, Baniulis D, Cramer WA, Faull KF, Whitelegge JP. Post-translational modifications of integral membrane proteins resolved by top-down Fourier transform mass spectrometry with collisionally activated dissociation. Mol Cell Proteomics. 2010 May;9(5):791-803. doi: 10.1074/mcp.M900516-MCP200. Epub 2010 Jan 21. PubMed PMID: 20093275; PubMed Central PMCID: PMC2871414.
Cramer WA, Yamashita E, Baniulis D, Hasan SS. The cytochrome b6f complex of oxygenic photosynthesis. In: Handbook of Metalloproteins USA: Wiley; 2010.
Baniulis D, Yamashita E, Whitelegge JP, Zatsman AI, Hendrich MP, Hasan SS, Ryan CM, Cramer WA. Structure-function, stability, and chemical modification of the cyanobacterial cytochrome b6f complex from Nostoc sp. PCC 7120. J Biol Chem. 2009 Apr 10;284(15):9861-9. doi: 10.1074/jbc.M809196200. Epub 2009 Feb 2. PubMed PMID: 19189962; PubMed Central PMCID: PMC2665108.
Baniulis D, Yamashita E, Zhang H, Hasan SS, Cramer WA. Structure-function of the cytochrome b6f complex. Photochem Photobiol. 2008 Nov-Dec;84(6):1349-58. doi: 10.1111/j.1751-1097.2008.00444.x. Review. PubMed PMID: 19067956.
Hasan SS, Singh S, Parikh RY, Dharne MS, Patole MS, Prasad BL, Shouche YS. Bacterial synthesis of copper/copper oxide nanoparticles. J Nanosci Nanotechnol. 2008 Jun;8(6):3191-6. doi: 10.1166/jnn.2008.095. PubMed PMID: 18681067.
Hasan SS, Ashraf GM, Banu N. Galectins - Potential targets for cancer therapy. Cancer Lett. 2007 Aug 8;253(1):25-33. doi: 10.1016/j.canlet.2006.11.030. Epub 2007 Jan 5. Review. PubMed PMID: 17207926.
HASAN LAB@UMB