LSR II Cleaning
Embedded Files
Compensation and Unmixing Theories
Cytek Aurora
Aurora Panel Design Template (Please use this table format to communicate with flow core. Dr. Fan's 23, 29 color panels are here)
Spectral Cytometry Panel Publications
Full spectrum flow cytometry and mass cytometry: A 32-marker panel comparison
Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry
Optimized Multicolor Panels
Technical Note
Apoptosis
Cytometry in Cell Necrobiology Revisited. Cytometry A 2010
Apoptosis and beyond: cytometry in studies of programmed cell death. Methods Cell Biol. 2011
Measurement and Characterization of Apoptosis by Flow Cytometry. Curr. Protoc. Cytom. 2016 (full text through library)
DNA Staining
YouTube Tutorial: Cell Cycle Analysis using FCSExperss 7 (Important to use Mathematical Models)
Proliferation Assay
The Use of CFSE to Monitor Lymphocyte Proliferation. J. Vis. Exp. 2010 (video)
Monitoring Lymphocyte Proliferation with CFSE. Nat. Protoc. 2007
Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Current Protoc. Cytom. 2013
Can request through the library. This one includes the alternative dye, Cell Trace Violet, less toxic and free up the FITC/GFP detector.
Imaging Cytometry
Overview of Unique Features (YouTube video)
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