Tribolium protocols klingler lab 

Histology, in situ stainings

• embryo fixation and devitellinization of embryos

• two-colour in situ hybridization

• RNA probes for in situ hybridization

• Fuchsin-staining, benzmix embedding

• Antibody staining of non-devitellinized embryos

Genetics

• working with beetles (link to Beeman lab)

• genetic maps of Tribolium (link to Beeman lab)

• visible mutants in Tribolium (link to Beeman lab)

• working with embryonic lethals: Berghammer 1999 + Supplement

RNAi, morpholino knock down

• parental RNAi

• morpholino oligos

Transgenics and CRISPR knock-ins

• germline transformation

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