Main publications  

Roadblocks of Urinary EV Biomarkers: Moving Toward the Clinic. Droste M, Puhka M, van Royen ME, Ng MSY, Blijdorp C, Alvarez-Llamas G, Borràs FE, Büscher AK, Bussolati B, Dear JW, Falcón-Pérez JM, Giebel B, Grange C, Hoorn EJ, Leivo J, Lenassi M, Llorente A, Lucien F, Mertens I, Mischak H, Pink D, Tertel T, Tiwari S, Vizio DD, Yuen PST, Zarovni N, Jenster G, Burger D, Martens-Uzunova ES, Erdbrügger U. J Extracell Vesicles. 2025 Jul;14(7):e70120. doi: 10.1002/jev2.70120. PMID: 40673839.

Urinary extracellular vesicles as a monitoring tool for renal damage in patients not meeting criteria for chronic kidney disease. Anfaiha-Sanchez M, Santiago-Hernandez A, Lopez JA, Lago-Baameiro N, Pardo M, Martin-Blazquez A, Vazquez J, Ruiz-Hurtado G, Barderas MG, Segura J, Ruilope LM, Martin-Lorenzo M, Alvarez-Llamas G. J Extracell Biol. 2024 Sep 17;3(9):e170. doi: 10.1002/jex2.170.

PMID: 39290459.

Urine beyond electrolytes: diagnosis through extracellular vesicles. Anfaiha-Sanchez M, Lago-Baameiro N, Santiago-Hernandez A, Martin-Blazquez A, Pardo M, Marta ML, Alvarez-Llamas G.Nefrologia (Engl Ed). 2024 Jul-Aug;44(4):503-508. doi:10.1016/j.nefroe.2023.05.020. Epub 2024 Jul 25. PMID: 39054239.

The Urinary Glycopeptide Profile Differentiates Early Cardiorenal Risk in Subjects Not Meeting Criteria for Chronic Kidney Disease. Santiago-Hernandez A, Martin-Lorenzo M, Gómez-Serrano M, Lopez JA, Martin-Blazquez A, Vellosillo P, Minguez P, Martinez PJ, Vázquez J, Ruiz-Hurtado G, Barderas MG, Sarafidis P, Segura J, Ruilope LM, Alvarez-Llamas G.  Int J Mol Sci. 2024 Jun 26;25(13):7005. doi: 10.3390/ijms25137005. PMID: 39000114.

Analysis of Vascular Smooth Muscle Cells from Thoracic Aortic Aneurysms Reveals DNA Damage and Cell Cycle Arrest as Hallmarks in Bicuspid Aortic Valve Patients. Martin-Blazquez A, Martin-Lorenzo M, Santiago-Hernandez A, Heredero A, Donado A, Lopez JA, Anfaiha-Sanchez M, Ruiz-Jimenez R, Esteban V, Vazquez J, Aldamiz-Echevarria G, Alvarez-Llamas G.  J Proteome Res. 2024 Apr 9. doi: 10.1021/acs.jproteome.3c00649. Epub ahead of print. PMID: 38594816. 

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches. Welsh JA, et al. J Extracell Vesicles. 2024 Feb;13(2):e12404. doi: 10.1002/jev2.12404. PMID: 38326288. 

Spatial metabolomics identifies LPC(18:0) and LPA(18:1) in advanced atheroma with translation to plasma for cardiovascular risk estimation. Jianhua Cao, Marta Martin-Lorenzo, Kim van Kuijk, Elias Wieland, Marion J. Gijbels Britt S. R. Claes, Angeles Heredero, Gonzalo Aldamiz-Echevarria, Ron M.A. Heeren, Pieter Goossens, Judith Sluimer, Benjamin Balluff, Gloria Alvarez-Llamas. Arteriosclerosis, Thrombosis, and Vascular Biology 2024 Mar;44(3):741-754. doi: 10.1161/ATVBAHA.123.320278. Epub 2024 Feb 1. PMID: 38299357.

Metabolomics Analysis of Urinary Extracellular Vesicles by Nuclear Magnetic Resonance and Liquid Chromatography-Mass Spectrometry. Martin-Lorenzo M, Molero D, Alvarez-Llamas G. Methods Mol Biol. 2023;2668:57-68. doi: 10.1007/978-1-0716-3203-1_6. PMID: 37140790. 

Prevention of cardiorenal damage: importance of albuminuria. Ruilope LM, Ortiz A, Lucia A, Miranda B, Alvarez-Llamas G, Barderas MG, Volpe M, Ruiz-Hurtado G, Pitt B. Eur Heart J. 2023 Apr 1;44(13):1112-1123. doi: 10.1093/eurheartj/ehac683. PMID: 36477861. 

Donor liquid biopsy and outcomes in kidney transplantation. Miriam Anfaiha-Sanchez, Emilio Rodrigo Calabia, Alberto Ortiz, Marta Martin-Lorenzo, Gloria Alvarez-Llamas. Clinical Kidney Journal. Oct 15;16(3):447-455. doi: 10.1093/ckj/sfac227. PMID: 36865017; PMCID: PMC9972840.

Prevention of cardiorenal damage: importance of albuminuria. Ruilope LM, Ortiz A, Lucia A, Miranda B, Alvarez-Llamas G, Barderas MG, Volpe M, Ruiz-Hurtado G, Pitt B. Eur Heart J. 2022 Dec 7:ehac683. doi: 10.1093/eurheartj/ehac683.

Evidence of chronic kidney injury in patients not meeting KDIGO criteria for chronic kidney disease. Alvarez-Llamas G, Santiago-Hernandez A, Ruilope LM. Clin Kidney J. 2022 Jan 12;15(7):1217-1220. doi: 10.1093/ckj/sfac007.

Metabolic Alterations Identified in Urine, Plasma and Aortic Smooth Muscle Cells Reflect Cardiovascular Risk in Patients with Programmed Coronary Artery Bypass Grafting. Santiago-Hernandez A, Martinez PJ, Agudiez M, Heredero A, Gonzalez-Calero L, Yuste-Montalvo A, Esteban V, Aldamiz-Echevarria G, Martin-Lorenzo M, Alvarez-Llamas G. Antioxidants (Basel). 2021 Aug 27;10(9):1369. doi:10.3390/antiox10091369.

TCA Cycle and Fatty Acids Oxidation Reflect Early Cardiorenal Damage in Normoalbuminuric Subjects with Controlled Hypertension. Santiago-Hernandez A, Martin-Lorenzo M, Martin-Blazquez A, Ruiz-Hurtado G, Barderas MG, Segura J, Ruilope LM, Alvarez-Llamas G.  Antioxidants (Basel). 2021 Jul 9;10(7):1100. doi: 10.3390/antiox10071100. 

Early renal and vascular damage within the normoalbuminuria condition. Santiago-Hernandez A, Martin-Lorenzo M, Martínez PJ, Gómez-Serrano M, Lopez JA, Cannata P, Esteban V, Heredero A, Aldamiz-Echevarria G, Vázquez J, Ruiz-Hurtado G, Barderas MG, Segura J, Ruilope LM, Alvarez-Llamas G. J Hypertens. 2021 Nov 1;39(11):2220-2231. doi: 10.1097/HJH.0000000000002936. 

Urinary Spermidine Predicts and Associates with In-Hospital Acute Kidney Injury after Cardiac Surgery. Martin-Lorenzo M, Ramos-Barron A, Gutierrez-Garcia P, Martin-Blazquez A, Santiago-Hernandez A, Rodrigo Calabia E, Gomez-Alamillo C, Alvarez-Llamas G.Antioxidants (Basel). 2021 Jun 2;10(6):896. doi: 10.3390/antiox10060896. 

Non-syndromic thoracic aortic aneurysm: cellular and molecular insights. Martin-Blazquez A, Heredero A, Aldamiz-Echevarria G, Martin-Lorenzo M, Alvarez-Llamas G. J Pathol. 2021 Jul;254(3):229-238. doi: 10.1002/path.5683. 

Analysis of urinary exosomal metabolites identifies cardiovascular risk signatures with added value to urine analysis. Agudiez M, Martinez PJ, Martin-Lorenzo M, Heredero A, Santiago-Hernandez A, Molero D, Garcia-Segura JM, Aldamiz-Echevarria G, Alvarez-Llamas G. BMC Biol. 2020 Dec 14;18(1):192. doi: 10.1186/s12915-020-00924-y. 

Urinary metabolic signatures reflect cardiovascular risk in the young, middle-aged, and elderly populations. Martinez PJ, Agudiez M, Molero D, Martin-Lorenzo M, Baldan-Martin M, Santiago-Hernandez A, García-Segura JM, Madruga F, Cabrera M, Calvo E, Ruiz-Hurtado G, Barderas MG, Vivanco F, Ruilope LM, Alvarez-Llamas G. J Mol Med (Berl). 2020 Nov;98(11):1603-1613. doi: 10.1007/s00109-020-01976-x. 

Molecular signatures of atherosclerotic plaques: An up-dated panel of protein related markers. Eslava-Alcon S, Extremera-García MJ, González-Rovira A, Rosal-Vela A, Rojas-Torres M, Beltran-Camacho L, Sanchez-Gomar I, Jiménez-Palomares M, Alonso-Piñero JA, Conejero R, Doiz E, Olarte J, Foncubierta-Fernández A, Lozano E, García-Cozar FJ, Rodríguez-Piñero M, Alvarez-Llamas G, Duran-Ruiz MC.J Proteomics. 2020 Jun 15;221:103757. doi: 10.1016/j.jprot.2020.103757.

Differential metabolic profile associated with the condition of normoalbuminuria in the hypertensive population. Santiago-Hernandez A, Martinez PJ, Martin-Lorenzo M, Ruiz-Hurtado G, Barderas MG, Segura J, Ruilope LM, Alvarez-Llamas G. Nefrologia (Engl Ed). 2020 Jul-Aug;40(4):440-445. doi: 10.1016/j.nefro.2019.10.007. 

Diabetes-mediated promotion of colon mucosa carcinogenesis is associated with mitochondrial dysfunction. Del Puerto-Nevado L, Santiago-Hernandez A, Solanes-Casado S, Gonzalez N, Ricote M, Corton M, Prieto I, Mas S, Sanz AB, Aguilera O, Gomez-Guerrero C, Ayuso C, Ortiz A, Rojo F, Egido J, Garcia-Foncillas J, Minguez P, Alvarez-Llamas G; DiabetesCancerConnect Consortium. Mol Oncol. 2019 Sep;13(9):1887-1897. doi: 10.1002/1878-0261.12531. 

Urine Haptoglobin and Haptoglobin-Related Protein Predict Response to Spironolactone in Patients With Resistant Hypertension. Martin-Lorenzo M, Martinez PJ, Baldan-Martin M, Lopez JA, Minguez P, Santiago-Hernandez A, Vazquez J, Segura J, Ruiz-Hurtado G, Vivanco F, Barderas MG, Ruilope LM, Alvarez-Llamas G. Hypertension. 2019 Apr;73(4):794-802. doi:10.1161/HYPERTENSIONAHA.118.12242.

Identification of six cardiovascular risk biomarkers in the young population: A promising tool for early prevention. Atherosclerosis. Martínez PJ, Baldán-Martín M, López JA, Martín-Lorenzo M, Santiago-Hernández A, Agudiez M, Cabrera M, Calvo E, Vázquez J, Ruiz-Hurtado G, Vivanco F, Ruilope LM, Barderas MG, Alvarez-Llamas G. 2019 Mar;282:67-74. doi: 10.1016/j.atherosclerosis.2019.01.003.

Molecular evidence of field cancerization initiated by diabetes in colon cancer patients. Del Puerto-Nevado L, Minguez P, Corton M, Solanes-Casado S, Prieto I, Mas S, Sanz AB, Gonzalez-Alonso P, Villaverde C, Portal-Nuñez S, Aguilera O, Gomez-Guerrero C, Esbrit P, Vivanco F, Gonzalez N, Ayuso C, Ortiz A, Rojo F, Egido J, Alvarez-Llamas G, Garcia-Foncillas J; DiabetesCancerConnect Consortium. Mol Oncol. 2019 Apr;13(4):857-872. doi: 10.1002/1878-0261.12438.

Advances in understanding the role of angiotensin-regulated proteins in kidney diseases. Expert Rev Proteomics. Sanz AB, Ramos AM, Soler MJ, Sanchez-Niño MD, Fernandez-Fernandez B, Perez-Gomez MV, Ortega MR, Alvarez-Llamas G, Ortiz A. 2019 Jan;16(1):77-92. doi: 10.1080/14789450.2018.1545577.

Urine metabolomics insight into acute kidney injury point to oxidative stress disruptions in energy generation and H2S availability. Martin-Lorenzo M, Gonzalez-Calero L, Ramos-Barron A, Sanchez-Niño MD, Gomez-Alamillo C, García-Segura JM, Ortiz A, Arias M, Vivanco F, Alvarez-Llamas G. J Mol Med (Berl). 2017 Dec;95(12):1399-1409. doi: 10.1007/s00109-017-1594-5.

Citric Acid Metabolism in Resistant Hypertension: Underlying Mechanisms and Metabolic Prediction of Treatment Response. Martin-Lorenzo M, Martinez PJ, Baldan-Martin M, Ruiz-Hurtado G, Prado JC, Segura J, de la Cuesta F, Barderas MG, Vivanco F, Ruilope LM, Alvarez-Llamas G. Hypertension. 2017 Nov;70(5):1049-1056. doi: 10.1161/HYPERTENSIONAHA.117.09819.

Immune system deregulation in hypertensive patients chronically RAS suppressed developing albuminuria. Martin-Lorenzo M, Gonzalez-Calero L, Martinez PJ, Baldan-Martin M, Lopez JA, Ruiz-Hurtado G, de la Cuesta F, Segura J, Vazquez J, Vivanco F, Barderas MG, Ruilope LM, Alvarez-Llamas G. Sci Rep. 2017 Aug 21;7(1):8894. doi: 10.1038/s41598-017-09042-2. Erratum in: Sci Rep. 2018 Mar

2;8(1):4154. 

Urinary exosomes reveal protein signatures in hypertensive patients with albuminuria. Gonzalez-Calero L, Martínez PJ, Martin-Lorenzo M, Baldan-Martin M, Ruiz-Hurtado G, de la Cuesta F, Calvo E, Segura J, Lopez JA, Vázquez J, Barderas MG, Ruilope LM, Vivanco F, Alvarez-Llamas G. Oncotarget. 2017 Jul 4;8(27):44217-44231. doi: 10.18632/oncotarget.17787.

Hypertensive patients exhibit an altered metabolism. A specific metabolite signature in urine is able to predict albuminuria progression. Gonzalez-Calero L, Martin-Lorenzo M, Martínez PJ, Baldan-Martin M, Ruiz-Hurtado G, Segura J, de la Cuesta F, Barderas MG, Ruilope LM, Vivanco F, Alvarez-Llamas G. Transl Res. 2016 Dec;178:25-37.e7. doi: 10.1016/j.trsl.2016.07.003.

Urinary Kininogen-1 and Retinol binding protein-4 respond to Acute Kidney Injury: predictors of patient prognosis? Gonzalez-Calero L, Martin-Lorenzo M, Ramos-Barron A, Ruiz-Criado J, Maroto AS, Ortiz A, Gomez-Alamillo C, Arias M, Vivanco F, Alvarez-Llamas G. Sci Rep. 2016 Jan 21;6:19667. doi: 10.1038/srep19667. 

Urinary alpha-1 antitrypsin and CD59 glycoprotein predict albuminuria development in hypertensive patients under chronic renin-angiotensin system suppression. Gonzalez-Calero L, Martin-Lorenzo M, de la Cuesta F, Maroto AS, Baldan-Martin M, Ruiz-Hurtado G, Pulido-Olmo H, Segura J, Barderas MG, Ruilope LM, Vivanco F, Alvarez-Llamas G. Cardiovasc Diabetol. 2016 Jan 16;15:8. doi:

10.1186/s12933-016-0331-7. 

Cytoskeleton deregulation and impairment in amino acids and energy metabolism in early atherosclerosis at aortic tissue with reflection in plasma. Martin-Lorenzo M, Gonzalez-Calero L, Maroto AS, Martinez PJ, Zubiri I, de la Cuesta F, Mourino-Alvarez L, Barderas MG, Heredero A, Aldamiz-Echevarría G, Vivanco F, Alvarez-Llamas G. Biochim Biophys Acta. Mol Basis Dis 2016 Apr;1862(4):725-732. doi:

10.1016/j.bbadis.2015.12.006.

Molecular histology of arteries: mass spectrometry imaging as a novel ex vivo tool to investigate atherosclerosis. Martin-Lorenzo M, Alvarez-Llamas G, McDonnell LA, Vivanco F. Expert Rev Proteomics. 2016;13(1):69-81. doi: 10.1586/14789450.2016.1116944. 

KLK1 and ZG16B proteins and arginine-proline metabolism identified as novel targets to monitor atherosclerosis, acute coronary syndrome and recovery. Martin-Lorenzo M, Zubiri I, Maroto AS, Gonzalez-Calero L, Posada-Ayala M, de la Cuesta F, Mourino-Alvarez L, Lopez-Almodovar LF, Calvo-Bonacho E, Ruilope LM, Padial LR, Barderas MG, Vivanco F, Alvarez-Llamas G. Metabolomics. 2015;11(5):1056-1067. doi: 10.1007/s11306-014-0761-8. 

 Lipid and protein maps defining arterial layers in atherosclerotic aorta. Martin-Lorenzo M, Balluff B, Maroto AS, Carreira RJ, van Zeijl RJ, Gonzalez-Calero L, de la Cuesta F, Barderas MG, Lopez-Almodovar LF, Padial LR, McDonnell LA, Vivanco F, Alvarez-Llamas G. Data Brief. 2015 Jun 24;4:328-31. doi: 10.1016/j.dib.2015.06.005. 

Molecular anatomy of ascending aorta in atherosclerosis by MS Imaging: Specific lipid and protein patterns reflect pathology.  Martin-Lorenzo M, Balluff B, Maroto AS, Carreira RJ, van Zeijl RJ, Gonzalez-Calero L, de la Cuesta F, Barderas MG, Lopez-Almodovar LF, Padial LR, McDonnell LA, Vivanco F, Alvarez-Llamas G. J Proteomics. 2015 Aug 3;126:245-51. doi: 10.1016/j.jprot.2015.06.005. 

Kidney tissue proteomics reveals regucalcin downregulation in response to diabetic nephropathy with reflection in urinary exosomes. Zubiri I, Posada-Ayala M, Benito-Martin A, Maroto AS, Martin-Lorenzo M, Cannata-Ortiz P, de la Cuesta F, Gonzalez-Calero L, Barderas MG, Fernandez-Fernandez B, Ortiz A, Vivanco F, Alvarez-Llamas G. Transl Res. 2015 Nov;166(5):474-484.e4. doi: 10.1016/j.trsl.2015.05.007. 

Exosomes: a potential key target in cardio-renal syndrome. Gonzalez-Calero L, Martin-Lorenzo M, Alvarez-Llamas G Front Immunol. 2014 Oct 8;5:465. doi: 10.3389/fimmu.2014.00465. 

30μm spatial resolution protein MALDI MSI: In-depth comparison of five sample preparation protocols applied to human healthy and atherosclerotic arteries. Martin-Lorenzo M, Balluff B, Sanz-Maroto A, van Zeijl RJ, Vivanco F, Alvarez-Llamas G, McDonnell LA. J Proteomics. 2014 Aug 28;108:465-8. doi: 10.1016/j.jprot.2014.06.013. 

Urine 2DE proteome analysis in healthy condition and kidney disease. Martin-Lorenzo M, Gonzalez-Calero L, Zubiri I, Diaz-Payno PJ, Sanz-Maroto A, Posada-Ayala M, Ortiz A, Vivanco F, Alvarez-Llamas G. Electrophoresis. 2014 Sep;35(18):2634-41. doi: 10.1002/elps.201300601. 

The authors reply: Kidney Int. 2014 May;85(5):1240. Alvarez-Llamas G, Vivanco F, Ortiz A. doi: 10.1038/ki.2014.39.

Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis. Zubiri I, Posada-Ayala M, Sanz-Maroto A, Calvo E, Martin-Lorenzo M, Gonzalez-Calero L, de la Cuesta F, Lopez JA, Fernandez-Fernandez B, Ortiz A, Vivanco F, Alvarez-Llamas G. J Proteomics. 2014 Jan 16;96:92-102. doi: 10.1016/j.jprot.2013.10.037.

Identification of a urine metabolomic signature in patients with advanced-stage chronic kidney disease. Posada-Ayala M, Zubiri I, Martin-Lorenzo M, Sanz-Maroto A, Molero D, Gonzalez-Calero L, Fernandez-Fernandez B, de la Cuesta F, Laborde CM, Barderas MG, Ortiz A, Vivanco F, Alvarez-Llamas G. Kidney Int. 2014 Jan;85(1):103-11. doi: 10.1038/ki.2013.328. 

Proteomic analysis of urinary exosomes in cardiovascular and associated kidney diseases by two-dimensional electrophoresis and LC-MS/MS. Zubiri I, Vivanco F, Alvarez-Llamas G. Methods Mol Biol. 2013;1000:209-20. doi: 10.1007/978-1-62703-405-0_16. 

Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS. de la Cuesta F, Barderas MG, Calvo E, Zubiri I, Maroto AS, Lopez JA, Vivanco F, Alvarez-Llamas G. Methods Mol Biol. 2013;1000:81-90. doi: 10.1007/978-1-62703-405-0_7. 

Secretome analysis of atherosclerotic and non-atherosclerotic arteries reveals dynamic extracellular remodeling during pathogenesis. de la Cuesta F, Barderas MG, Calvo E, Zubiri I, Maroto AS, Darde VM, Martin-Rojas T, Gil-Dones F, Posada-Ayala M, Tejerina T, Lopez JA, Vivanco F, Alvarez-Llamas G. J Proteomics. 2012 Jun 6;75(10):2960-71. doi: 10.1016/j.jprot.2011.12.005. 

Metabolomic profiling for identification of novel potential biomarkers in cardiovascular diseases. Barderas MG, Laborde CM, Posada M, de la Cuesta F, Zubiri I, Vivanco F, Alvarez-Llamas G. J Biomed Biotechnol. 2011;2011:790132. doi: 10.1155/2011/790132.

Recent advances in atherosclerosis-based proteomics: new biomarkers and a future perspective. Alvarez-Llamas G, de la Cuesta F, Barderas ME, Darde V, Padial LR, Vivanco F. Expert Rev Proteomics. 2008 Oct;5(5):679-91. doi: 10.1586/14789450.5.5.679.