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Throughout the day, Dr. Louis removed ticks from two other dogs. Dr. Louis was concerned that the ticks might be carrying pathogens (viruses and other disease-causing microorganisms) that could harm the dogs. These pathogens would be living inside of the tick’s body and are transmitted to the dog during the bite. Dr. Louis packaged the ticks into sealed plastic bags and sent them to the local veterinary laboratory for analysis.
Throughout the day, Dr. Louis removed ticks from two other dogs. Dr. Louis was concerned that the ticks might be carrying pathogens (viruses and other disease-causing microorganisms) that could harm the dogs. These pathogens would be living inside of the tick’s body and are transmitted to the dog during the bite. Dr. Louis packaged the ticks into sealed plastic bags and sent them to the local veterinary laboratory for analysis.
To test for pathogens, a lab technician places DNA samples from the ticks into different wells on an electrophoresis gel. Gel electrophoresis separates DNA fragments (pieces) on the basis of size. Large DNA fragments move slowly through the gel. Small DNA fragments move quickly through the gel.
To test for pathogens, a lab technician places DNA samples from the ticks into different wells on an electrophoresis gel. Gel electrophoresis separates DNA fragments (pieces) on the basis of size. Large DNA fragments move slowly through the gel. Small DNA fragments move quickly through the gel.
Tick Pathogen Detection Video
Tick Pathogen Detection Video
Tick Pathogen Detection Protocol
1. DNA stain is made by adding 20 ml of tap water to a plastic bag labeled DNA Stain.
2. The plastic bag is sealed and gently swirled so the DNA stain dissolves.
3. The Simulated Electrophoresis Gel is then added to the plastic bag.
4. The plastic bag is sealed, then placed on a flat surface.
5. After a few minutes a color change can be observed showing the banding patterns (pink bands) for each of the dogs (Max, Daisy, Lainey).
(Note: The video above does not have audio)
1. After watching the Tick Pathogen Detection video (above), click HERE to view the results of the Tick Pathogen Detection. Then determine if any of the ticks removed from Dr. Louis' patients were carrying pathogens. Record the banding pattern of the simulated electrophoresis gel in your Digital Lab Notebook.
1. After watching the Tick Pathogen Detection video (above), click HERE to view the results of the Tick Pathogen Detection. Then determine if any of the ticks removed from Dr. Louis' patients were carrying pathogens. Record the banding pattern of the simulated electrophoresis gel in your Digital Lab Notebook.
You can continue below, once you record your observations and findings in your Digital Lab Notebook!
Tick Pathogen Standard Key
Tick Pathogen Standard Key
The Tick Pathogen Standard Key displays banding patterns for common tick-borne pathogens. Compare the banding patterns in the tick samples to the Tick Pathogen Standard Key.
The Tick Pathogen Standard Key displays banding patterns for common tick-borne pathogens. Compare the banding patterns in the tick samples to the Tick Pathogen Standard Key.
2. Use your digital lab notebook to record the information needed to complete the laboratory form. The form will be used to return the results to Dr. Louis. Follow the prompts in your digital lab notebook by placing an "X" in the box where pathogens were detected. Then move on to step 3.
2. Use your digital lab notebook to record the information needed to complete the laboratory form. The form will be used to return the results to Dr. Louis. Follow the prompts in your digital lab notebook by placing an "X" in the box where pathogens were detected. Then move on to step 3.
3. Which pet’s owners should be notified of possible pathogen transmission to their dog? Record your answer in your digital lab notebook.
3. Which pet’s owners should be notified of possible pathogen transmission to their dog? Record your answer in your digital lab notebook.
4. During a follow up visit with Daisy, Dr. Louis was informed that Daisy’s owner was walking through the woods with Daisy when she was bitten by the tick. Dr. Louis was concerned that Daisy’s owner may also have been exposed to the pathogen through a tick bite. List some of the symptoms that Daisy’s owner might exhibit if he was exposed to this pathogen. Record your list in your digital lab notebook.
4. During a follow up visit with Daisy, Dr. Louis was informed that Daisy’s owner was walking through the woods with Daisy when she was bitten by the tick. Dr. Louis was concerned that Daisy’s owner may also have been exposed to the pathogen through a tick bite. List some of the symptoms that Daisy’s owner might exhibit if he was exposed to this pathogen. Record your list in your digital lab notebook.
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