Mutant Strains

Lots and Lots of Mutant Strains...

Molecular Genetics classes in the Microbiology Department at the Ohio State University (taught by Kathleen Sandman) generated more than 25,000 random transposon mutants of Rhodobacter sphaeroides strain 2.4.1 that grow on a defined minimal medium with either succinate or D/L-lactate as the carbon source. The work was presented by Emily Smith and Jordan Allen at the 2010 Denman Undergraduate Research Forum. A copy of their poster is available here.

Minimal Media Composition (1 L)

22.4 mM NH₄Cl, 0.8 mM MgSO₄, 0.5 mM CaCl₂, 15 mM potassium phosphate buffer (pH 6.7), 1 ml/L vitamin solution (100 mg cyanocobalamin, 300 mg pyridoxamine-2HCl, 100 mg calcium-D(+)-pantothenate, 200 mg thiamindichlorine, 200 mg nicotinic acid, 80 mg 4-aminobenzoic acid, 20 mg D-(+)-biotin) 10 ml/L trace element solution (500 mg ethylenediamine tetracetic acid (EDTA, disodiun salt), 300 mg FeSO₄ · 7H₂O, 3 mg MnCl₂ · 4H₂O, 5 mg CoCl₂ · 6H₂O, 1 mg CuCl₂ ·2H₂O, 2 mg NiCl₂ · 6H₂O, 3 mg Na₂MoO₄ · 2H2O, 5 mg ZnSO₄ · 7H₂O, and 2 mg H₃BO₃ per liter (pH3) and 10 mM of either sodium succinate or sodium lactate.

Table of Transposon Mutants

The following table, assembled by Jordan Allen and Birgit Alber, lists thos mutants for which transposon insertion has been mapped and a stock culture is available upon request. Please contact Birgit Alber (alber.8@osu.edu) with any requests for stock cultures.