Pathway Tracing Methods
The table below lists neuronal pathway tracers that have been methodologically employed in experiments designed to determine the organization of neuronal connections. For each pathway tracer, its direction of transport and transport properties are indicated (in so far as these are known). A general validity rating (1-7 / lowest-highest) is assigned to each pathway tracer based on several criteria (including its uptake and transport properties, and sensitivity). Refer to the document below the table for additional details and information (best viewed in pop out window).
Header Image: Photo illustration of the iontophoretic method that is often used in pathway tracing experiments. A glass micropipette is filled with an ionic solution of pathway tracer molecules. Application of an electric current of appropriate polarity results in ejection of the tracer molecules from the tip of the micropipette (adapted from Purves, R. D. 1979).