Research 

JAN 2023 - Jan 2024

Master's thesis (research intern) & Laboratory Assistant

Prof. Erik Alexanderssn & Dr. Sajeevan Radha Sivarajan team , [Supervisor: Prof.Filippo Geuna, Unimi]

Swedish University of Agricultural Sciences(SLU) 

"Understanding the role of WRKY transcription factors in early blight disease of potato caused by Alternaria solani"

Techniques & learning: PCR, Gradient PCR, qRT-PCR, molecular cloning, Invitro culture, RNA isolation, Primer design, DNA extraction, sterile techniques, Agroinfiltration, fungal inoculation, disease assessment, Virus-Induced Gene silencing(VIGS), Bioinformatics: sequence analysis, genomic data analysis,  Cis-regulatory elements analysis, Protein coexpression network analysis, phylogenetic relation analysis, synteny analysis etc.  

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MAY 2019 - JAN 2020

Research intern

Nepal Agriculture genetic resource center (Genebank), Kathmandu Nepal 

"Characterization and regeneration of Phaseolus vulgaris and Abelmoschus esculentus genotypes of Nepal"

Techniques and learning: Experimental design, seed quality test, seed germination test, phenotyping and characterization of the crops, disease assessment and scoring, tissue culture techniques, DNA extraction, PCR, tissue culture techniques, long-term storage of regenerated seeds 

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Research Skills:

Molecular techniques: 

DNA & RNA extraction, PCR, Gradient PCR, qRT-PCR, agarose gel electrophoresis,  cDNA synthesis, Molecular cloning technique, agroinfiltration

Bioinformatics:

Genome-wide analysis, familiarization with databases, sequence analysis, the study of domain pattern, gene structure and motif analysis, phylogenetic analysis, synteny analysis, gene duplication events, cis-regulatory element analysis, Protein co-expression network analysis, Snapgene, TBtool and its functions, MEGA11, UGENE, EMBOSS suit, GenALEx etc.

Programming and data analysis:

R, Python (Basics), JASP

Other:

Proposal and report writing, Presentation skills, familiarisation with Google online tools, Microsoft Office, Basic graphic design for scientific reports