Due to the high demand of biofuel, corn belt is dynamically expanding towards its periphery in the midwestern USA. As the center of the corn belt has become saturated with corn and other bio feedstock production, a peripheral shift makes Kansas a potential area for biofuel production. In fact, northeastern Kansas has experienced an increase in agricultural production in recent decades. However, the water quality impacts of biofuel-based landuse scenarios (increased rotational corn-soybeans production) at watershed scale is largely understudied. This study focuses 2924 km2 Delaware River Watershed located in northeastern Kansas which drains into Perry Lake, a regionally important reservoir further drains into the Kansas River. Our 15-years (2006-2021) land use change analysis revealed a significant grassland to corn-soy conversion rate (15% reduction and 12% increase respectively) which is causing return of CRP land into farming. Based on land use change intensity and accessibility of the location, 30 sampling sites were selected for seasonal synoptic sampling from Fall 2020 to Summer 2022 to capture the effect of land use change on water quality. Based on the experimental results of water quality parameters (total phosphorous, soluble reactive phosphorous, nitrate and other environmentally relevant anions, total and dissolved organic carbon, total suspended solid, organic matter percentage etc.) and the location of the sites, the whole watershed was delineated into 30 subwatershed and water quality parameters were used to correlate with the land use change occurred in the subwatershed scale. In the next phase of our study, a water quality model will be developed to determine the extent of biofuel expansion based on the water quality data and land use analysis. It can reveal sustainable extent of renewable fuel growth towards the periphery of corn belt and whether Kansas can be the next hub of biofuel expansion.