We and our collaborators have used short-read sequencing to identify SNPs, indels, and structural variations relative to the C57BL/6J mouse reference genome. The strains that have been sequenced and are in our variation catalog are:

The sample accession codes are listed here. The sequence variation can be queried via our query tool. For bulk download, the sequencing reads are available in BAM format from our ftp site and the variations are available in VCF format on our ftp site. All of the variation data has been published and can be used without restriction. The primary citation for the resource is:


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NOTE: These assembled chromosomes are released as unpublished, preliminary and incomplete sequences and as such they have not yet been submitted to the accessioned in the public genome sequence repositories (INSDC). The assembled sequences will be fully accessioned in public repositories at the time of publication. These data are released in accordance with the Fort Lauderdale agreement and Toronto agreements. As producers of these data we reserve the right to be the first to publish a genome-wide analysis of the data we have generated. The pre-publication data that we release are embargoed for publication except for analyses of single chromosomes in single strains or single gene loci across multiple strains. We strongly encourage researchers to contact us (mousegenomes@gmail.com) if there are any queries about referencing or publishing analysis based on pre-publication data. We expect to accession and publish the genome sequences and strain specific gene annotation in mid-late 2016.

This sequencing centre plans on publishing the completed and annotated sequences in a peer-reviewed journal as soon as possible. Permission of the principal investigator should be obtained before publishing analyses of the sequence/open reading frames/genes on a chromosome or genome scale. See our data sharing policy.

Now I want to do the same with a mouse. However, Android is grabbing the mouse and presenting a mouse-cursor. When I write a device-filter with the vendor and product ID of the mouse, I do not get it like with the SpaceNavigator (strangely, both are HID -- I get no cursor with the SpaceNavigator).

As soon as your Application claims the Mouse (as a USB HID device while being Host), Android should hide the cursor and you can read the raw data. This should work on stock android, but your device has to support USB Host mode and a USB OTG cable will be needed to connect the mouse.

I am completely new to this type of work, and really need help in understanding how to approach this (I've never used CIBERSORT before). My supervisor has given me an excel file full of RNAseq data from mouse tumors. There are a total of 12 samples (6 controls & 6 tumors) with the gene names on the left column. She wants me to figure out if we can use CIBERSORTx on mouse data. I've followed the CIBERSORTx tutorials with their practice files, and while it appears very straightforward regarding human data, I feel I don't have enough of a thorough understanding of the concepts to translate it to mouse data. I hope that makes sense. I tried imputing cell fractions on the mouse data using the signature matrix files already uploaded by CIBERSORT and it gives an error. I made sure to clean up the mouse data (removed any duplicates or blanks, left only the gene names on the left, the samples to the right, and saved as a .txt file). I tried replacing the mouse gene names with the human equivalents, but still get the same error:

I am new to cybersort as well. You can use mouse bulk RNA-seq data, but blindly using "signature matrix files already uploaded by CIBERSORT" would be weird to do in my opinion. Try to find mouse single cell datasets that are relevant to your bulk RNA-seq data using celldex and scRNAseq packages. Then use this to create a signature matrix. There are many for mouse (maybe even more than human) but pick one that is going to provide useful cell type information for deconvolution of bulk data. I would also read through this([SOLVED] How to build a table with gene expression per cell type with Seurat ?) post thoroughly and also read the attached links in the comments of that post.

The Mouse Phenome Database (MPD; ) is a widely accessed and highly functional data repository housing primary phenotype data for the laboratory mouse accessible via APIs and providing tools to analyze and visualize those data. Data come from investigators around the world and represent a broad scope of phenotyping endpoints and disease-related traits in nave mice and those exposed to drugs, environmental agents or other treatments. MPD houses rigorously curated per-animal data with detailed protocols. Public ontologies and controlled vocabularies are used for annotation. In addition to phenotype tools, genetic analysis tools enable users to integrate and interpret genome-phenome relations across the database. Strain types and populations include inbred, recombinant inbred, F1 hybrid, transgenic, targeted mutants, chromosome substitution, Collaborative Cross, Diversity Outbred and other mapping populations. Our new analysis tools allow users to apply selected data in an integrated fashion to address problems in trait associations, reproducibility, polygenic syndrome model selection and multi-trait modeling. As we refine these tools and approaches, we will continue to provide users a means to identify consistent, quality studies that have high translational relevance.

Both individuals and organizations that work with arXivLabs have embraced and accepted our values of openness, community, excellence, and user data privacy. arXiv is committed to these values and only works with partners that adhere to them.

The mammalian brain is composed of many cell populations that differ based on their molecular, morphological, electrophysiological and functional characteristics. Classifying these cells into types is one of the essential approaches to defining the diversity of the brain's building blocks. This project seeks to characterize cortical diversity at the cellular level for several neuroanatomical areas in both mouse and human. Additional data will be released regularly, building toward a complete picture of cellular diversity of the brain and how this diversity is conserved across species.

Single cell (or single nucleus) RNA sequencing (RNA-Seq) is a scalable approach to provide genome-wide expression profiles for thousands of cells. This data set includes single cell and nuclear transcriptomic profiles, assayed from human and mouse brain regions. Anatomical specificity is achieved by microdissecting tissue from defined brain areas, such as cortical layers or cell groups in LGd.

Abundant anatomical and physiological evidence supports the presence of at least 3 distinct types of principal or relay neurons in the dorsal lateral geniculate nucleus (dLGN), the brain region that conveys visual information from the mammalian retina to the primary visual cortex. This data set provides detailed transcriptional profiles of cells from this area, available for download. Cells derived from this area were compared across 3 species (human, macaque, and mouse) to assess cross-species correspondence based on transcriptional profiles.

The Allen Institute for Brain Science uses a unique approach to generate data, tools and knowledge for researchers to explore the biological complexity of the mammalian brain. This portal provides access to high quality data and web-based applications created for the benefit of the global research community.

The rise of full transcriptome acquisition technologies has fueled the rapid proliferation of molecular-level biological data. These large datasets require interpretation beyond the single-gene level to connect them to meaningful biology and clinical impacts. In 2003, we pioneered the gene set enrichment analysis (GSEA) approach1 to enable the identification of the coordinate activation or repression of sets of genes that share common biology, thereby distinguishing even subtle differences between phenotypes or cellular states. We first released our GSEA software and a companion resource of gene sets, the Molecular Signatures Database (MSigDB), in 2005 (ref. 2).

A.S.C. and J.M.R. developed the ortholog mapping procedure. C.J.B. and J.M.R. identified and curated the gene sets in the MP:Tumor collection; J.M.R. wrote the code to generate the gene sets. J.M.R. and C.J.B. produced the ortholog translated hallmarks collection. D.E. collected existing mouse gene sets from MSigDB. A.S.C. curated all other sets and collections and produced the underlying gene mapping data. A.S.C. and D.E. produced the complete MSigDB build. A.S.C., D.E. and H.T. designed the updates to the MSigDB website; D.E. implemented the updates. A.S.C., H.T. and J.P.M. wrote the manuscript with input and feedback from all authors. C.J.B. and J.P.M. provided oversight and guidance.

We developed a three-dimensional (3D) synthetic animated mouse based on computed tomography scans that is actuated using animation and semirandom, joint-constrained movements to generate synthetic behavioral data with ground-truth label locations. Image-domain translation produced realistic synthetic videos used to train two-dimensional (2D) and 3D pose estimation models with accuracy similar to typical manual training datasets. The outputs from the 3D model-based pose estimation yielded better definition of behavioral clusters than 2D videos and may facilitate automated ethological classification.

This work was supported by a Canadian Institutes of Health Research (CIHR) FDN-143209, NIH R21, a Fondation Leducq grant, Brain Canada for the Canadian Neurophotonics Platform and a Canadian Partnership for Stroke Recovery Catalyst grant to T.H.M. H.R. is supported by grants from Natural Sciences and Engineering Research Council of Canada (NSERC). D.X. was supported in part by funding provided by Brain Canada, in partnership with Health Canada, for the Canadian Open Neuroscience Platform initiative. This work was supported by computational resources made available through the NeuroImaging and NeuroComputation Centre at the Djavad Mowafaghian Centre for Brain Health (RRID SCR_019086). N.L.F. is supported by an NSERC Discovery Grant. Micro-CT imaging was performed at the UBC Centre for High-Throughput Phenogenomics, a facility supported by the Canada Foundation for Innovation, British Columbia Knowledge Development Foundation and the UBC Faculty of Dentistry. We thank E. Koch and L. Raymond of the UBC Djavad Mowafaghian Centre for Brain Health for the mouse open field video. e24fc04721

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