Wang, Z., Min, X., Xiao, S. H., Johnstone, S., Romanow, W., Meininger, D., Xu, H., Liu, J., Dai, J., An, S., Thibault, S., & Walker, N. (2013). Molecular basis of sphingosine kinase 1 substrate recognition and catalysis. Structure, 21(5). https://doi.org/10.1016/j.str.2013.02.025
Pyne, N. J., Tonelli, F., Lim, K. G., Long, J. S., Edwards, J., & Pyne, S. (2012). Sphingosine 1-phosphate signalling in cancer. Biochemical Society Transactions, 40(1), 94–100. https://doi.org/10.1042/BST20110602
Vettorazzi, M., Angelina, E., Lima, S., Gonec, T., Otevrel, J., Marvanova, P., Padrtova, T., Mokry, P., Bobal, P., Acosta, L. M., Palma, A., Cobo, J., Bobalova, J., Csollei, J., Malik, I., Alvarez, S., Spiegel, S., Jampilek, J., & Enriz, R. D. (2017). An integrative study to identify novel scaffolds for sphingosine kinase 1 inhibitors. European Journal of Medicinal Chemistry, 139. https://doi.org/10.1016/j.ejmech.2017.08.017
Capra, J. A., & Singh, M. (2007). Predicting functionally important residues from sequence conservation. Bioinformatics, 23(15). https://doi.org/10.1093/bioinformatics/btm270
Valdar, W. S. J. (2002). Scoring residue conservation. Proteins: Structure, Function and Genetics, 48(2). https://doi.org/10.1002/prot.10146
Ashkenazy, H., Abadi, S., Martz, E., Chay, O., Mayrose, I., Pupko, T., & Ben-Tal, N. (2016). ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules. Nucleic Acids Research, 44(W1). https://doi.org/10.1093/NAR/GKW408
Zhao, X., Lu, Z., Na, Y., & and Shu, M. (n.d.). Identification of potential SPHK1 inhibitors based on structural optimization by molecular simulation. Journal of Biomolecular Structure and Dynamics, 1–10. https://doi.org/10.1080/07391102.2025.2479849
Alkafaas, S. S., Elsalahaty, M. I., Ismail, D. F., Radwan, M. A., Elkafas, S. S., Loutfy, S. A., Elshazli, R. M., Baazaoui, N., Ahmed, A. E., Hafez, W., Diab, M., Sakran, M., El-Saadony, M. T., El-Tarabily, K. A., Kamal, H. K., & Hessien, M. (2024). The emerging roles of sphingosine 1-phosphate and SphK1 in cancer resistance: a promising therapeutic target. In Cancer Cell International (Vol. 24, Issue 1). https://doi.org/10.1186/s12935-024-03221-8
Chen, H., Haddadi, N., Zhu, X., Hatoum, D., Chen, S., Nassif, N. T., Lin, Y., & McGowan, E. M. (2022). Expression Profile of Sphingosine Kinase 1 Isoforms in Human Cancer Tissues and Cells: Importance and Clinical Relevance of the Neglected 1b-Isoform. Journal of Oncology, 2022. https://doi.org/10.1155/2022/2250407
Jin, L., Zhu, J., Yao, L., Shen, G., Xue, B. xin, & Tao, W. (2023). Targeting SphK1/2 by SKI-178 inhibits prostate cancer cell growth. Cell Death and Disease, 14(8). https://doi.org/10.1038/s41419-023-06023-4
Software and Tools:
Sequence alignment:
Van Rossum, G., & Drake, F. L. (2009). Python 3 Reference Manual. CreateSpace.
Scientific illustration:
BioRender. (n.d.). BioRender, from https://biorender.com
Sequence Retrieval:
COSMIC. (2025). SPHK1 gene mutations in prostate cancer samples. Retrieved April 15, 2025, from https://cancer.sanger.ac.uk/cosmic/gene/samples?all_data=&coords=AA%3AAA&dr=&end=471&gd=&id=286412&ln=SPHK1&seqlen=471&sn=prostate&src=gene&start=1
UniProt. (2025). SPHK1 – Sphingosine kinase 1 (Human) – Q9NYA1. Retrieved April 1, 2025, from https://www.uniprot.org/uniprotkb/Q9NYA1/entry
UniProt. (2025). SPHK1 – Sphingosine kinase 1 (Mouse) – Q8CI15. Retrieved April 1, 2025, from https://www.uniprot.org/uniprotkb/Q8CI15/entry
UniProt. (2025). SPHK1 – Sphingosine kinase 1 (Rat) – Q91V26. Retrieved April 1, 2025, from https://www.uniprot.org/uniprotkb/Q91V26/entry
UniProt. (2025). SPHK1 – Sphingosine kinase 1 (Chicken) – A0A1D5P1F1. Retrieved April 1, 2025, from https://www.uniprot.org/uniprotkb/A0A1D5P1F1/entry
Multiple Sequence Alignment:
EMBL-EBI. (2025). Clustal Omega – Multiple Sequence Alignment Tool. Retrieved April 1, 2025, from https://www.ebi.ac.uk/Tools/msa/clustalo/
Variant Impact Prediction:
Ensembl. (2025). Homo sapiens gene: ENSG00000176170 (SPHK1) – Gene summary. Retrieved April 15, 2025, from https://useast.ensembl.org/Homo_sapiens/Gene/Summary?g=ENSG00000176170
3D Structure Analysis:
Mirdita, M., Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). AlphaFold2.ipynb – ColabFold notebook [Jupyter notebook]. GitHub. https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2.ipynb
Schrödinger, LLC. (2015). The PyMOL Molecular Graphics System, Version 2.0. https://pymol.org