Tissue
Sectioning
Image Taken By Matt Chu.
When understanding and discovering the mechanisms behind factors that drive and halt proliferation, in vitro work can only go so far. The ability to replicate a treatment in a animal model is the best way to confirm the validity of what is being tested.
In order to do this the Sectioning team freezes hearts of rats, treated and wild type. These hearts are then thinly sliced into sections that are only few micrometers thick using a machine called a cryostat.
This team also heavily applies immunohistochemistry assays in order to be able to view fluorescence of different markers within the tissue.
Similar to the Cellular team, we also learn skills in image analysis with Fiji ImageJ, which is used to determine how effective a treatment is or how cells are acting within the tissue.
What You Can Learn:
Animal Handling and Maintenance (i.e. Rat)
Tissue Dissection
Histology
Immunohistochemistry
Microscopy
Fluorescence
Data Analysis
Oral and Written Scientific Communication
Stained section of a Cardiomyocyte. Image captured by Matt Chu
Raw TIFF file Fluorescent images of Cardiomyocytes (green), DAPI (Blue), Ki-67 (Red). Images taken by Matt Chu.