Molecular Biology

When trying to understand and control the mechanisms behind what drives proliferation as well as what causes the cardiomyocytes to stop dividing, molecular biology techniques are required to design and express constructs that would then be used by the cellular biology team can then use the product to test the validity of the construct in an in vitro assay. Techniques include Bacterial Cloning, Gene Silencing, Cell Marking, Gene Regulation.

What You Can Learn:

• Molecular Cloning

  • Bacterial Cultures

  • Plasmid Preparations

  • Combinatorial Assembly Methods (e.g. Gibson, Multi-Site) 

  • Sequencing

• Viral Packaging

• DNA/RNA/Protein Isolation and Purification

• PCR and Primer Design

• Gel Electrophoresis

• Western Blotting

• Data Analysis

• Oral and Written Scientific Communication