Procedure

1. Preparation of samples

a. Normal Blood Color Accuracy Setup and Test

i. Open Corel DRAW X7 and navigate to the

tools section

ii. Under the tools tab, open the color wheel

window. Clear any values already loaded

and/or reset the tool

iii. Input value 138 into the Red, or R section

iv. Input value 3 into the Green, or G section

v. Input 3 into the Blue, or B section

vi. Click save.

vii. Afterwards, open the display and save the

resulting color square, as determined by the

values inputted

viii. Save the image as “BloodStandard.”

ix. Upload into a .docx document using Microsoft

Word

x. Load standard 8 x 11 paper into the printer.

xi. Select print on the document

xii. Once printed, inspect the document for any

marks, printing errors, or any other type of

disruption in the color pattern.

xiii. Set the clamp platform adjacent to the paper,

and orient the clamp horizontally

xiv. Depress the rear metal clip on the clamp

xv. Move the upper right edge of the color sensor

into the jaws of the clamp

xvi. Release the rear clip

xvii. Adjust as necessary to secure the color

sensor and assure that no movement or shift is

possible

xviii. Upon doing so, power on the desired

computer

xix. Load Arduino IDE

xx. Navigate to the “Sketch” tab

xxi. Open the Updatedcode.ino

xxii. Use the verify function

xxiii. Upon verification, select the upload and

compile function

xxiv. Attach the connecting end of the USB

cable to an available port on the desktop

xxv. Reload the code

xxvi. To check for functionality, observe a

flickering effect in two second intervals on the

LED light source affixed to the TCS34725 color

sensor

xxvii. After, navigate to the Tools tab in the

Arduino IDE

xxviii. Open the Serial Monitor

xxix. The sensor should begin producing

RGB values

xxx. After confirmation of RGB value input,

the Serial Port monitor should then be closed.

xxxi. Afterwards, take the printed out color

block and position it underneath the sensor.

Observe where the photodiode array is located

in relation to the paper. Make sure that the

LED and the array are positioned directly over

the paper

xxxii. Lower or raise the clamp until the

sensor is 1 inch away from the paper sample

xxxiii. Press and hold the reset button on the

Arduino board

xxxiv. Navigate to the Serial Monitor tool and

open the new tab

xxxv. Make sure that no data is being

recorded before the selected timeframe

xxxvi. Set a timer for 1 minute and position it

next to the sensor

xxxvii. Start the timer

xxxviii. Release the reset button on the sensor

xxxix. Allow for the data collection period to

run, and then at the 1 minute mark press the

reset button again. This will stop the Arduino

from collecting more data than specified in the

1 minute mark.

xl. Export the Serial Monitors data into a separate

notepad document and save it.

xli. Take a random set of RGB values to plug into

Corel DRAW X7

xlii. Plug in the values and open up the display

window and verify that the color matches

xliii. Save all related imagery in data not

already collected

b. Blood substitute samples

i. Measure out 40mL of water into beaker

ii. Inspect each of the sample containers for any

marks, cracks, or otherwise that might affect

the amount of liquid or the color sensors

readings

iii. Label the samples per at risk, not at risk

sample

iv. Set out the first sample for not at risk with

10mL of water

v. Measure out two drops of Substitute 1A dye for

the first sample and set aside

vi. Measure out 10mL of water into second not at

risk container

vii. Measure out two drops of Substitute 2A dye

for second sample and place next to first

sample

viii. Measure out 10 mL of water into first at risk

container

ix. Measure out 2 drops of Substitute 3B dye into

first at risk container and set aside

x. Measure out last 10mL of water from beaker

xi. Add 2 drops of Substitute 4B into second at

risk container

xii. Order according to dye and place printed

control sheet to farthest left

2. Preparation of Arduino Board

a. Attach USB connector cable to COM port on the

Arduino MAX 2560 board

b. Attach opposite end to USB port on selected

computer with Arduino IDE installed

c. Run ArduinoIDE.exe

d. Once opened, navigate to the file tab and select the

‘sketchbook’ subsection

e. Open prepared Updatedcode.ino

f. Use the verify tool to make sure that the import

process was correctly done

3. Preparation of TCS34725 color sensor

a. Attach the VCC wire end of the TCS34725 to the

extended F-F wire and hook the pin into the 5V port

b. Attach the SCL wire end of the TCS34725 to the

extended F-F wire and hook the pin into the 21SCL

port

c. Attach VCC wire end of the TCS34725 to the first

GND port on the Arduino board

4. Sample Testing

a. Using the wire holder, depress the rear end of the

clamps and hold them

b. Navigate the upper right hand edge of the prepared

TCS34725 within the clamps

c. Release the rear end of the clamps

d. Check to make sure that no form of movement is

possible for the TCS34725 within the clamps

e. Make sure that the photodiode array and the LED

system is not blocked by any other part

f. Connect the opposite end into the computer with the

Updatedcode.ino loaded

g. Select the compile and upload tool in the Arduino IDE

h. To begin, order the test strip panel in a horizontal

pattern relative to the location of the TCS34725

i. Adjust the TCS34725 to a height of ½ inch above the

surface

j. Set a timer for 15 seconds

k. Begin moving sample 1 under the TCS34725. Make

sure that the photodiode array is within the center for

maximum accuracy

l. In intervals of 15 seconds, do a trial of three runs over

sample 1

m. Collect the data and paste each run into a separate

document

n. Shift the panel until sample 2 is now under the

photodiode array part of the TCS34725

o. Repeat the 15 second interval trials and save them

according to the sample in separate documents each

p. Adjust the panel for sample 3’s analysis, and once

again set 3 separate 15 second trials and record the

data

q. Finally, adjust sample 4. Take care not to catch the

edge and make sure the outer edge of the LED

systems illumination is not reaching the edges.

r. Repeat the 3 separate 15 second trial tests.

s. Save the data.