Our Lab is focused on understanding the mechanism of autophagy in the mammalian system. Studies include understanding the membrane dynamics during the biogenesis of autophagosomes and investigation of consequent steps in autophagy. Autophagy has a role in a vast variety of human pathophysiological conditions including cancer. Inhibition of autophagy and lysosomal systems is being tested in clinical trials against several types of cancer. However, the molecular mechanism of the role of autophagy in cancer is poorly understood. Our lab works on delineating the mechanism by which autophagy regulates cancer progression. We also study the mechanism underlying the role of autophagy in conferring resistance to chemotherapies against colorectal, lung, and breast cancer. Our lab utilizes multiple microscopy-based techniques including super-resolution microscopy, electron microscopy, and confocal microscopy to study the membrane dynamics in autophagy. Moreover, unbiased proteomics studies, RNA-Seq, and metabolomics approaches are carried out to study the role of autophagy in cancer. We work with cancer cell lines, and mouse models and develop organoids from biopsies from cancer patients.