Research Articles

RESEARCH ARTICLES FROM LAB 

1. Raina, K., Modak, K., Premkumar, C., Joshi, G., Palani, D., Nandy, K., ... & Thummer, R. P. (2025). UTF1 Expression is Important for the Generation and Maintenance of Human iPSCs. Stem Cell Reviews and Reports, 1-13 (IF: 4.2).

2. Narayan G, Sen P, Nagotu S, Thummer RP (2023) Biological activity of recombinant human PDX1 protein produced from Escherichia coli. J Biochem Mol Toxicol (Wiley); 37(12): e23511, https://doi.org/10.1002/jbt.23511 (IF: 3.600)

3. Narayan G, Agrawal A, Sen P, Nagotu S, Thummer RP (2023) Production of bioactive human PAX4 protein from E. coli. The Protein Journal (Springer Nature), 42, 766–777, https://doi.org/10.1007/s10930-023-10143-3 (IF: 3.000)

4. Khyati R, Joshi G, Modak K, Premkumar C, Priyanka S, Rajesh P, Velayudhan SR, Thummer RP (2023) Generation and characterization of induced Pluripotent Stem Cell line IITGi001-A derived from adult human primary dermal fibroblasts. Stem Cell Research; 71:103159, https://doi.org/10.1016/j.scr.2023.103159 (IF: 1.200)

5. Haridhasapavalan KK, Sundaravadivelu PK, Voorkara U, Kaveeshwar V, Thummer RP (2023) Generation of recombinant version of a bioactive human MEF2C transcription factor from _E. coli_. In: Pandey, L.M., Gupta, R., Thummer, R.P., Kar, R.K. (eds) Healthcare Research and Related Technologies. NERC 2022. Springer Nature. https://doi.org/10.1007/978-981-99-4056-1_1, ISBN: 978-981-99-4055-4

6. Dey C, Raina R, Thummer RP (2023) Production of a bioactive recombinant human UTF1 protein from E. coli. In: Pandey, L.M., Gupta, R., Thummer, R.P., Kar, R.K. (eds) Healthcare Research and Related Technologies. NERC 2022. Springer Nature. https://doi.org/10.1007/978-981-99-4056-1_4, ISBN: 978-981-99-4055-4

7. Thool M, Sudhagar S, Thummer RP (2023) Soluble Expression and Purification of Biologically Active Human NANOG from Escherichia coli. In: Pandey, L.M., Gupta, R., Thummer, R.P., Kar, R.K. (eds) Healthcare Research and Related Technologies. NERC 2022. Springer Nature. https://doi.org/10.1007/978-981-99-4056-1_6, ISBN: 978-981-99-4055-4

8. Narayan G, Nagotu S, Thummer RP (2023) Heterologous Expression, Purification, and Biological Activity Determination of Codon-Optimized Human MAFA Transcription Factor from E. coli. In: Pandey, L.M., Gupta, R., Thummer, R.P., Kar, R.K. (eds) Healthcare Research and Related Technologies. NERC 2022. Springer Nature, Singapore. https://doi.org/10.1007/978-981-99-4056-1_9, ISBN: 978-981-99-4055-4

9.  Haridhasapavalan KK, Sundaravadivelu PK, Joshi N, Das NJ, Mohapatra A, Voorkara U, Kaveeshwar V*, Thummer RP* (2022) Generation of a recombinant version of a biologically active cell-permeant human HAND2 transcription factor from E. coli. Scientific Reports 12, 16129 (Springer Nature), https://doi.org/10.1038/s41598-022-19745-w * Equal contribution (IF: 4.996)

10. Haridhasapavalan KK, Das NJ, Thummer RP (2022) Generation of a transducible version of a bioactive recombinant human TBX5 transcription factor from E. coli. Current Research in Biotechnology, 4: 66-77 (Elsevier), https://doi.org/10.1016/j.crbiot.2022.01.004 (IF: N/A)

11. Bhattacherjee D, Raina K, Mandal TK, Thummer RP$, Bhabak KP$ (2022) Targeting Wnt/β-catenin signaling pathway in triple-negative breast cancer by benzylic organotrisulfides: Contribution of the released hydrogen sulfide towards potent anti-cancer activity. Free Radical Biology and Medicine (Elsevier), https://doi.org/10.1016/j.freeradbiomed.2022.08.029 $ Corresponding authors (IF: 8.101)

12.  Narayan G, Agrawal A, Gogoi R, Nagotu S, Thummer RP (2021) Protein production and purification of a codon-optimized human NGN3 transcription factor from E. coli. The Protein Journal 40(6), 891-906, (Springer Nature), https://doi.org/10.1007/s10930-021-10020-x (IF: 4000)

13. Dey C, Venkatesan V, Thummer RP (2021) Identification of optimal expression parameters and purification of a codon-optimized human GLIS1 transcription factor from Escherichia coli. Molecular Biotechnology 64, 42–56 (Springer Nature), https://doi.org/10.1007/s12033-021-00390-z (IF: 2.860)

14. Haridhasapavalan KK, Das NJ, Thummer RP (2021) Generation of a transducible version of a bioactive recombinant human TBX5 transcription factor from E. coli. bioRxiv, https://doi.org/10.1101/2021.09.04.458986 (IF: N/A)

15. Dey C*, Thool M*, Bhattacharyya S, Sudhagar S, Thummer RP (2021) Generation of biologically active recombinant human OCT4 protein from E. coli. 3 Biotech, 11(5): 207. (Springer Nature), *Equal contribution. https://doi.org/10.1007/s13205-021-02758-z (IF: 2.893)

16. Haridhasapavalan KK, Harsh Ranjan S, Bhattacharyya S, Thummer RP (2021) Soluble expression, purification, and secondary structure determination of human MESP1 transcription factor. Applied Microbiology and Biotechnology, 105(6): 2363-2376 (Springer Nature), https://doi.org/10.1007/s00253-021-11194-1 (IF: 5.560)

17. Thool M*, Dey C*, Sudhagar S, Thummer RP (2021) Generation of a recombinant stem cell-specific human SOX2 protein from Escherichia coli under native conditions. Molecular Biotechnology, 63(4): 327-338 (Springer Nature), *Equal contribution. https://doi.org/10.1007/s12033-021-00305-y (IF: 2.860)

18. Haridhasapavalan KK, Sundaravadivelu PK, Bhattacharyya S, Harsh Ranjan S, Raina K, Thummer RP (2021) Generation of cell-permeant recombinant human transcription factor GATA4 from E. coli. Bioprocess and Biosystems Engineering, 44(6): 1131-1146 (Springer Nature), https://doi.org/10.1007/s00449-021-02516-8 (IF: 3.434)

19. Narayan G, Sundaravadivelu PK, Agrawal A, Gogoi R, Nagotu S, Thummer RP (2021) Soluble expression, purification, and secondary structure determination of human PDX1 transcription factor. Protein Expression and Purification, 180: 105807 (Elsevier), https://doi.org/10.1016/j.pep.2020.105807 (IF: 2.025)

20.  Haridhasapavalan KK*, Sundaravadivelu PK*, Thummer RP (2020) Codon optimization, cloning, expression, purification, and secondary structure determination of human ETS2 transcription factor. Molecular Biotechnology, 62(10): 485-494 (Springer Nature), *Equal contribution. https://doi.org/10.1007/s12033-020-00266-8 (IF: 2.860)

21.  Munst B*, Thier MC*, Winnemoller D*, Helfen M, Thummer RP$, Edenhofer F$ (2016) Nanog induces suppression of senescence through downregulation of p27KIP1 expression. Journal of Cell Science 129(5): 912-920 (The Company of Biologists (United Kingdom)), https://doi.org/10.1242/jcs.167932 $ Corresponding authors (IF: 5.235)

22. Peitz M*, Munst B*, Thummer RP*, Helfen M, Edenhofer F (2014) Cell-permeant recombinant Nanog protein promotes pluripotency by inhibiting endodermal specification. Stem Cell Research 12(3): 680-689 (Elsevier), *Equal contribution. https://doi.org/10.1016/j.scr.2014.02.006 (IF: 1.587)

23. Kadari A, Lu M, Li M, Thileepan S, Thummer RP, Guyette N, Chu V, Edenhofer F (2014) Excision of a viral reprogramming cassette by Cre protein transduction enables rapid and efficient derivation of transgene-free human induced pluripotent stem cells. Stem Cell Research and Therapy 5(2): 1-10 (BioMed Central Ltd; Part of Springer Nature), https://doi.org/10.1186/scrt435 (IF: 8.079)

24. Sekaran T*, Thummer RP*, Edenhofer F (2013) Exploring the neural reprogrammome using bioinformatics approaches. Journal of Stem Cell Research and Therapy 3(3): 1-6 (Longdom), *Equal contribution. http://dx.doi.org/10.4172/2157-7633.1000146

25. Thummer RP, Drenth-Diephuis LJ, Eggen BJ (2012) Constitutive UTF1 expression interferes with ES and EC cell differentiation. Journal of Stem Cell Research and Therapy 2(4): 1-7 (Longdom), http://dx.doi.org/10.4172/2157-7633.1000127

26. Thummer RP, Drenth-Diephuis LJ, Carney KE, Eggen BJ (2010) Functional characterization of single-nucleotide polymorphisms in the human undifferentiated embryonic-cell transcription factor 1 gene. DNA and Cell Biology (Mary Ann Liebert, Inc.) 29(5): 241-248, https://doi.org/10.1089/dna.2009.0981 (IF: 3.550)

27. Kooistra SM*, van den Boom V*, Thummer RP, Johannes F, Wardenaar R, Tesson BM, Veenhoff LM, Fusetti F, O'Neill LP, Turner BM, de Haan G, Eggen BJ (2010) UTF1 regulates ESC chromatin organization and gene expression. Stem Cells 28(10): 1703-1714 (Wiley), *Equal contribution. https://doi.org/10.1002/stem.497 (IF: 5.845)

28. Kooistra SM, Thummer RP, Eggen BJ (2009) Characterization of human UTF1, a chromatin-associated protein with repressor activity expressed in pluripotent cells. Stem Cell Research 2(3): 211-218 (Elsevier), https://doi.org/10.1016/j.scr.2009.02.001 (IF: 1.587)

29. Thummer RP, Campbell MP, Dean MK, Frusher MJ, Scott PD, Reynolds CA (2005) Entropy and Oligomerization in GPCRs. Journal of Molecular Neuroscience 26(2-3): 113-122 (Springer Nature), https://doi.org/10.1385/jmn:26:2-3:113 (IF: 2.866)

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 Other Research Articles (in collaboration) 

30. Yadav, M., Singh, H. V., Basu, S., Thummer, RP (2025), & Chatterjee, S. Impact of the Arrangement Modes of the Antimicrobial Motifs WWR‐7 and its Analogs in CAMPs on Their Therapeutic Properties. Chemistry–An Asian Journal, e70330 (IF: 3.3).

31. Chaudhari H, Das A, Singh H, Ghosh A, Kulkarni R, Bhuyan MK, Thummer RP (2025) Common path off-axis digital holographic flow cytometry based on lateral shearing using beam splitter. In Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VII (Vol. 13571, pp. 276–282). SPIE.

32. Chaudhari H, Das A, Singh HV, Ghosh A, Kulkarni R, Bhuyan MK, Thummer RP (2025) Lateral shearing common-path digital holographic microscopy with iterative reconstruction. Applied Optics, 64(22), 6285–6291 (IF: 1.7).

33. Sarkar T, Rajalakshmi VS, KR R, Thummer RP, Chatterjee S (2025) Serum-Stable, Cationic, α-Helical AMPs to Combat Infections of ESKAPE Pathogens and C. albicans. ACS Appl Bio Mater. https://doi.org/10.1021/acsabm.5c00126 (IF: 4.5)

34. Yadav M, Singh T, Singh HV, Thummer RP, Chatterjee S (2025) Salt-Tolerant, Protease-Stable and Non-Resistance Developing Cationic AMPs for Combatting Planktonic MRSA and its Biofilms. ACS Med Chem Lett. https://doi.org/10.1021/acsmedchemlett.5c00121 (IF: 3.9)

35. Chaudhari H, Sundaravadivelu PK, Kulkarni R, Bhuyan MK, Thummer RP (2025) Cumulative learning based segmentation aided cell mixtures classification in digital holographic microscopy. Opt Laser Technol; 181: 112029. https://doi.org/xxxxxx (IF: X.XXX)

36. Bhabak KP, Badirujjaman M, Thummer RP (2024) Esterase‐responsive Self‐immolative Prodrugs for the Sustained Delivery of the Anticancer Drug 5‐Fluorouracil with Turn‐on Fluorescence. Chem Asian J; e202400846. https://doi.org/xxxxxx (IF: X.XXX)

37. Chatterjee S, Sarkar T, Sundaravadivelu PK, Thummer RP, Satpati P (2024) De novo Design of Tryptophan containing Broad‐Spectrum Cationic Antimicrobial Octapeptides. ChemMedChem; e202400566. https://doi.org/xxxxxx (IF: X.XXX)

38. Chaudhari H, Kulkarni R, Bhuyan MK, Sundaravadivelu PK, Thummer R (2024) Machine-learning-based classification of co-cultured cells from the phase images in digital holographic microscopy. Unconventional Optical Imaging IV; 12996: 87-96. SPIE. https://doi.org/xxxxxx (IF: X.XXX)

39. Mehak, Thummer RP, Pandey LM, Srivatsan TS (2024) Engineered Iron-Oxide Based Nanomaterials for Magnetic Hyperthermia. Adv Mater Emerg Appl; 440-463. Bentham Science Publishers. https://doi.org/xxxxxx (IF: X.XXX)

40. Sarkar T, Vignesh SR, Sehgal T, Ronima KR, Thummer RP, Satpati P, Chatterjee S (2024) Development of protease resistant and non-cytotoxic Jelleine analogs with enhanced broad spectrum antimicrobial efficacy. Biochim Biophys Acta Biomembr; 1866(6): 184336. https://doi.org/xxxxxx (IF: X.XXX)

41. Sarkar T, Ghosh S, Sundaravadivelu PK, Pandit G, Debnath S, Thummer RP, Satpati P, Chatterjee S (2024) Mechanism of protease resistance of D-amino acid residue containing cationic antimicrobial heptapeptides. ACS Infectious Diseases (ACS Publications), 10, 2, 562–581, https://doi.org/10.1021/acsinfecdis.3c00491 (IF: 4.870)

42. Chetia M, Sarkar T, Yadav M, Dey C, Sundaravadivelu PK, Thummer RP, Chatterjee S (2024) Tuning of hydrophobic-hydrophilic balance for the development of a salt-tolerant and protease-resistant lipopeptide AMP. New Journal of Chemistry (Royal Society of Chemistry), 48, 2764-2777, https://doi.org/10.1039/d3nj04815b (IF: 3.300)

43. Chaudhari H, Kulkarni R, Sundaravadivelu PK, Thummer RP, Bhuyan MK (2024) Dimensionality reduction technique based phase aberration compensation and spurious fringe removal in off-axis digital holographic microscopy. Optics and Lasers in Engineering (Elsevier), 107853, https://doi.org/10.1016/j.optlaseng.2023.107853 (IF: 4.600)

44. Chaudhari H, Kulkarni R, Sundaravadivelu PK, Thummer RP, Bhuyan MK (2024) Digital hologram reconstruction algorithm based on Fractional Fourier transform in non-telecentric digital holographic microscopy. Optics Letters (Optica Publishing Group), 49(2), 182-185, https://doi.org/10.1364/OL.504723 (IF: 3.600)

45.  Deori NM, Infant T, Thummer RP, Nagotu S (2022) Characterization of the multiple domains of Pex30 involved in subcellular localization of the protein and regulation of peroxisome number. Cell Biochemistry and Biophysics (Springer Nature), https://doi.org/10.1007/s12013-022-01122-z (IF: 2.989)

46. Abu S, Bhattacherjee D, Pallavi B, Thummer RP, Bhabak KP (2022) Stimuli-responsive prodrug of non-steroidal anti-inflammatory drug diclofenac: self-immolative drug release with turn-on near-infrared fluorescence. Chemical Communications (Royal Society of Chemistry), https://doi.org/10.1039/d2cc02132c (IF: 6.065)

47. Banerjee K, Bhattacherjee D, Raina K, Thummer RP, Bhabak KP (2022) Benzimidazole-based ionic and non-ionic organoselenium compounds: Innovative synthetic strategies, structural characterizations and preliminary anti-proliferative activities. New Journal of Chemistry (Royal Society of Chemistry), https://doi.org/10.1039/d2nj01322c (IF: 3.925)

48. Deori NM, Infant T, Sundaravadivelu PK, Thummer RP, Nagotu S (2022) Pex30 undergoes phosphorylation and regulates peroxisome number in Saccharomyces cerevisiae. Molecular Genomics and Genetics, 297: 573–590 (Springer Nature), https://doi.org/10.1007/s00438-022-01872-8 (IF: 2.980)

$ Corresponding authors.

* These authors have contributed equally to this manuscript.

Impact factor (IF) based on year 2023 (Clarivate Analytics).