The last two decades witnessed a significant alteration in the design, fabrication, and selection of biomaterials in tissue engineering applications. Recent advancements in 3D printing have made it possible to print biocompatible materials and living cells into complex 3D functional tissue constructs. Therefore, macroporous anisotropic scaffolds with hierarchical structure using bioactive, biomimetic materials are suitable for developing various in-vitro tumor models. Osteosarcoma (OS) is one of the primary malignant bone tumors that primarily impact the age group of children and affects the long bones of the extremities. Currently available techniques, like surgical and chemotherapy approaches, are ineffective in reducing tumor growth and metastasis. Hence, mimicking such diseased model in vitro condition and investigating with new OS anticancer drugs have attracted attention to understand the mechanisms of tumor progression, invasion, and metastasis, which direct toward effective cancer therapies. Here, we propose a synthetic tissue analogue by 3D printing of organoid harbouring stimulated design under dynamic cell culture conditions.