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In this section, you will learn how use the light microscope to obtain clear and well-contrasted images.
You may use the following set of slides and click on the simulation to practice your microscopy skills:
Miscroscopy Simulator.pptxThe use of microscopes is a critical skill in A-level Practical. You are required to know how to:
prepare the specimen,
mount it on a slide,
stain [sometimes],
observe and
draw the visible biological structures.
Below, we shall learn skills 1 - 4. Please read the following and practice often.
1. Preparing the specimen
(a) Cutting specimen (such as onion, potato)
Cutting techniques.mp4(b) Obtaining inner epidermal layer of onion scale
Sample Preparation - Obtaining inner epidermal layer.m4v(c) Obtaining outer epidermal layer of onion scale
Sample Preparation - Obtaining outer epidermal layer.m4v2. Mount specimen on slide
(a) Via wet mount
Preparation of Slide_Wet Mount.m4v(b) Via smears [e.g. blood samples]
Preparation of Slide_Smears.m4v(c) Via hanging drop method [e.g. live samples]
Preparation of Slide - Hanging Drop Method.mp44. Observe specimen - Use of Microscope
(a) Focusing to obtain a clear image
(b) Adjusting the brightness and contrast of image
(c) Adjusting focus of eyepiece graticule
Preparation of Microscope_Adjusting focus of eyepiece graticule.m4v(d) Adjusting the orientation of eyepiece graticule
Preparation of Microscope_Adjusting orientation of eyepiece graticule.m4vPage updated
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