The 3rd quarterly edition of RuBisCo newsletter presents an immersive read on the fascinating possibilities offered and milestones achieved by the ever-evolving field of metabolomics. This field provides the researchers with the ability to take a peek into a system’s physiological states and the effects of environmental, genetic and lifestyle factors on them. On an application front, metabolomics provides an avenue for research and development for personalized medicine by identification of disease-specific biomarkers for prognosis, diagnosis and treatment. Metabolomics helps greatly in elucidating various metabolic pathways implicated with pharmaceutical compounds and study their effect on the pathways as well as on a system in general, thus improving the quality of drug development, drug efficacy and safety profiles. As an evolving field, metabolomics faces several challenges but as the understanding and technologies get advanced, metabolomics along with other -omics technologies give researchers a holistic understanding of a biological system, standing at the forefront of modern biological sciences research.