The more time the mice spend closer to the walls, the more anxious they are. I am going to look at the videos in 10-second intervals. If the mouse seems to be exploring the outer perimeter/walls of the enclosure, they will get +1, and if they don't, they will get -1. I measured the exploration/interaction of the perimeter based on whether the mouse stopped moving to interact with the wall-if they stopped moving for a second, I counted it as +1, but if they were moving the whole time, I considered it -1. The total score will indicate how anxious I think the mouse was during the study. A higher score means the mouse was more anxious, and a lower score means it was less anxious.
PT_OF_M.11.15 Total Score: 14
PT_OF_M.11.16 Total Score: 10
PT_OF_F.11.19 Total Score: 12
PT_OF_F.11.14 Total Score: 9
I think the mouse from the last video was the least anxious, and the mouse from the first video was the most anxious of the four.