A Novel Isolate Methylocystis sp. NLS7 as a promising candidate for industrial production of PHA from methane

Author: Eleni Moutsoglou, Department of Chemical and Biological Engineering

Mentor: Dr. David Salem, CNAM-Bio Center and Department of Chemical and Biological Engineering, in affiliation with the BuG ReMeDEE Initiative

Mentor: Dr. Rajesh Sani, CNAM-Bio Center and Department of Chemical and Biological Engineering, in affiliation with the BuG ReMeDEE Initiative

Recent interest in the mitigation of greenhouse gases has contributed to an increased amount of research in the field of methanotrophy. Studies have demonstrated that methane-oxidizing bacteria have the ability to utilize methane as their sole carbon and energy source to produce polyhydroxyalkanoates, or PHAs, which can be modified to produce renewable plastics. Methylosinus trichosporium OB3b and Methylocystis parvus OBBP have been presented as the most feasible bacteria to be used as industrial workhorses for polyhydroxyalkanoate production from methane. We have shown that a novel isolate Methylocystis sp. NLS7 has robust growth on methane, comparable to that of the OB3b and OBBP previously published. In addition, FTIR has confirmed the presence of polyhydroxyalkanoate purified from the biomass of NLS7. Once the yield of PHA from NLS7 is further optimized, the methanotroph will become a promising candidate for the large-scale industrial production of polyhydroxyalkanoates.