Plants communicate through chemical secretions in the soil in order to receive necessary nutrients from microorganisms. Microorganisms offer support in many ways to plants, specifically in terms of enhanced growth, tolerance to stress, and disease resistance. However, due to genetically inherited features, native plants are far less vulnerable to issues such as disease than invasive plants, which does not account for the ability of invasive plant species to completely take over environments. In order to combat the advantage that native plants would have, invasive plants are more dependent on microbes to act in place of generations of protection (Kowalski et al., 2019). Due to this idea, we hypothesized that areas in the forest with high levels of invasive plants would have higher bacteria populations than areas in the forest with high levels of native plants. We used the correlation between protozoa and bacteria that was recognized during our preliminary E.S.S.R.E microclimate research to predict that invasive plant plots would also show higher levels of protozoa than native plant plots. By testing the soil for bacteria population, pH, and protozoa population in areas of the forest with high-density native and invasive areas, we were able to confirm this. To test this hypothesis, we conducted experimental extractions from two sets of two 4x4 meter plots, one to a high density of native plants, and one to invasive plants, our hypothesis was not fully supported. The difference between bacteria levels in invasive and native plots were significantly different with higher levels in the invasive plot, our hypothesis about protozoa was not supported, however. We believed that, due to the fact that protozoa eat bacteria, there would be a positive correlation- meaning that when bacteria populations are higher, protozoa populations would also be higher. This led us to believe that invasive plots would have increased protozoa populations.