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RESEARCH AREA

Epigenetic modulators play a key role in regulating gene expression as well as DNA damage response. These modulators include histone modifying enzymes and ATP-dependent chromatin remodeling proteins.

We seek to understand:

Role of ATP-dependent chromatin remodeling proteins in Candida albicans: Candida albicans is an opportunistic fungus that causes Candidiasis. We are investigating the role of ATP-dependent chromatin remodeling proteins in the pathogenesis and virulence of the organism.
Regulating the regulators: How are the epigenetic regulators regulated? Our studies show that these epigenetic regulators, in fact, regulate each other. For example, we have shown that SMARCAL1, a distant member of the ATP-dependent chromatin remodeling protein family, and BRG1, another member of the family, mutually co-regulate each other in mammalian cells, and thereby regulate the DNA damage response pathway. In C. albicans, we find that, Rtt109 mediates H3K56 acetylation which is needed for Fun30 expression. In turn, Fun30, an ATP-dependent chromatin remodeler, regulates the transcription of Rtt109. Both together mediate DNA damage response pathway.

ONGOING PROJECTS

Exploring the role of RAD5 in Candida albicans.

Exploring the regulation of the SWI/SNF complex in Candida albicans

TEACHING

M.Sc. Courses:

LS 403A-Genetics

LS 505A-Human Genetics

LS 567- Eukaryotic Gene expression

Ph.D. Courses:

LS 637A- Gene expression in Eukaryotes

LAB MEMBERS

current memBERS

Anusha Ghosh

Akanksha Kanojia

Rajan Kushwaha

Sanju

Ketki Patne

Post-doc

M.Sc. Dissertation.

Uzma Khan

Intern

Akansha Arora

PUBLICATIONS

Kanojia, A., Roy, G., Madhubala, R., and Muthuswami, R., Interplay between DOT1L and HDAC1 regulates Leishmania donovani infection in human THP-1 cells (2024). Acta Tropica.
Kaur, M., Mehta, R., Muthuswami, R., Mallick, B.N., Noradernaline enhances Na-K ATPase subunit expression by HuR-induced mRNA stabilization and their transportation t the cell surface through PLC and PKC mediated pathway: implications with REMS-loss associated disorders (2024). J. Neurochem. Epublished.
Tandon, S., Puri, M., Bharath, Y., Choudhary, U.M., Mohapatra, D.K., Muthuswami, R., and Madhubala, R., In vitro screening of natural product-based compounds for leishmanicidal activity (2023). J. Parasitic Dis. 1-15.
Puri, M., Brar, H.K., Madan, E., Srinivasan, R., Rawat, K., Gorthi, S.S., Kumari, G., Sah, R.K., Ojha, S.B., Panigrahi, S., Dhangadamajhi, G., Muthuswami, R., Singh, S., and Madhubala, R., Rapid diagnosis of Plasmodium falciparum malaria using a point-of care loop-mediated isothermal amplification device (2022). Front. Cell. Infect. Microb. 12:961832.
Dutta, A., Das, A., Bisht, D., Arya, V., and Muthuswami, R., PLK-1 interacting checkpoint helicase, PICH, mediates cellular oxidative stress response (2022). Epigenomes. 6:36.
Bisht, D., Patne, K., Rakesh, R., and Muthuswami, R., On the interaction between SMARCAL1 and BRG1 (2022) Front. Cell Dev. Biol. 10: 870815.
Maurya, P.K., Garai, P., Goel, K., Bhatt, H., Goyal, A., Dewasthale, S., Gupta, M., Dutta, A., Arya, V., and Muthuswami, R., Fun30 and Rtt109 mediate epigenetic regulation of the DNA damage response pathway in C. albicans (2022). J. of Fungi 8: 559.
Brar, H.K., Roy, G., Madan, E., Madhubala R., Muthuswami, R., Chromatin-remodeling factor BRG1 is a negative modulator of L. donovani in IFNγ stimulated and infected THP-1 cells (2022). Front. Cell. Infect. Microb. 392: 860058.
Arya, V., Dutta, A., and Muthuswami, R. CD spectroscopy in studies of DNA-protein interactions (2022). J. Vis. Exp. Feb 10;(180). doi: 10.3791/63147
Tandon, S., Muthuswami, R., and Madhubala, R., Role of two aminoacyl-tRNA synthetase associated proteins (Endothelial Monocyte Activating Peptides 1 and 2) of Leishmania donovani in chemotaxs of human monocytes (2021). Acta Trop. 224: 106128 doi: 10.1016/j.actatropica.2021.106128. Epub 2021 Sep 9

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