Our research team aims to integrate LC-MS/MS-based omics technologies, including proteomics, peptidomics, and metabolomics analyses, to uncover the peptide signaling pathways and regulatory mechanisms involved in plant-nematode interactions. We have identified a set of evolutionarily conserved long-distance migrating peptides that can regulate lignin biosynthesis and enhance plant immunity, thereby contributing to the development of a novel biotechnological approach for nematode control. Peptides are recognized as primary mediators of intercellular communication across various physiological processes in multicellular organisms. Plant peptide hormones have been demonstrated to systematically modulate developmental processes in response to both abiotic and biotic stressors. Recent findings underscore the significance of peptides in parasitic nematode infections through host mimicry. Hence, investigating the roles of peptides in the systemic mediation of plant-nematode interactions not only advances our understanding of nematode infection physiology but also offers an alternative perspective for devising new biotechnological strategies for nematode control.