PAKAR Laboratories LTD

[PAKAR Laboratories LTD]

Active pharmaceutical and fine chemical library preparation and large scale production

Mission of the project

Driving drug discovery and development by providing Active pharmaceutical and fine chemical library and large scale production

PLG Technology Platforms Innovation

Updates👑

PLG Technology Platforms Innovation

Updates👑

Molecular Super Intelligence in the

age of Digital Biology🎯✅

💥1️⃣ Imagine a source of new molecules

with unprecedented chemical and

structural diversity.

📌Medicinal fungal fermentation

technology platform | MFFTP.

💥2️⃣Imagine a system of multi-tier

chemical separation so powerful that it

can generate over 132,000,000 fractions

at industrial scale.

📌Ultra-deep chemical analysis and

separation technology platform

| UDCASTP.

💥3️⃣Imagine large libraries of new

chemical entities of more than

1,000,000,000, capable of producing hits

against any biological target, any

biological mechanism and any biological

question.

📌 Libraries of active

pharmaceutical ingredients with

unprecedented chemical

structural diversity and

mechanisms of action | APILTP.

💥4️⃣Imagine a wide array bioassays to

interrogate huge numbers of biological

targets and biological mechanisms more

than 1,000,000 across all life forms.

📌Chemical Systems Biology using

multi-omics technologies

| CSB-MOT

🎁 Finally imagine employing these

powerful machines to produce data of

unprecedented depths and scope

unveiling master regulators and master

mechanisms leading to most definitive

and decisive solutions to global health

and one health challenges.

⛔Challenges such as cancer, neglected

tropical diseases, antimicrobial

resistance, emergence of new

hypervigilant pathogens, pandemics and

aging.

🆔️PAKARLab and 🆔️PAKARLabs our

vision and mission is audacious but

we will proceed inspite of formidable

odds.

🎯 PLG LLC Products Innovation Update🎁:

The BBC News reports a unique medical story of a woman

who suffered eye 👁️ problems for months.

It was solved only when a biopsy of eye fluid was analyzed

using metagenomics that a rare bacterium was detected.

She has been cured with a combination of antibiotics.

This illustrates the power of our approach toward the

fight against antimicrobial resistance and zoonotic

disease transmission and one health. We employ a

unique set of products and service innovations through:

📌The routine metagenomic surveys of poultry and livestock farms.

📌 AI-powered automation of data analysis and bespoke access to farmers.

📌 Provision of a wide variety of anti-bacterial feed supplements.

📌 Pre-emptive and preventive mode of fighting infections

and boosting productivity and food security.

✅ This will ensure a long lasting and sustainable

growth in the food supply and one health.

Anti-Infective Feed Supplements

Active Pharmaceutical Ingredients Libraries

Imagination and creativity makes it work beyond the limits.

Chemistry also works with simple and elegant techniques. Mastering the value of low cost methods paves the way for reaching higher heights.

Imagination and creativity makes it work beyond the limits.

Imagination is more important than knowledge: inventing a TLC sprayer.

We live by this idea daily in our small and struggling laboratory for chemical systems biology. Struggling yet bold with our ideas and ambitions.

This is a story of the power improvisation within low resources settings. For months now we have been trying to procure spraying device for thin layer chromatography. The device we wanted is a bit expensive and the procurement is taking forever.

So got to work to find a local replacement

which will work fine. All the options we tried fall apart after a few rounds of use due to the strong acid in the staining reagent.

So we went back to town and found this item that looks like it's made with durable plastic. It's used for spreading insecticides and cleaning solutions.

We could vouch for the spraying mechanism to survive so this quick thinking brother decided to separate the problem into two and limit the exposure of strong acid only to the nozzle side and spare the air pump.

He tried a long test tube first and then we thought of this little flask. At least it reduces the number of times we need to refill.

We tested it and worked beautifully. The nozzle is tunable which allows us to obtain super fine spraying. And it is very durable too. We have used it intensely for two months now and it's going strong.

Again our quick thinking brother decided to be over cautious. And he rinses the device with deionized water each time after use in order not to give the strong acid the chance to drift into the air pump side. The soft plastics that allows the piston to deliver the pressure will not survive.

It looks like with this strategy we can going to have this improvised device around for a long time. And costed us just about 7 USD instead 190 USD that would have taken forever to arrive.

Feel free and set one for your Laboratory if you don't have a good sprayer at hand. This is better than pouring the reagent over the plate or even dipping into a tank of reagent.

14 years of R&D

This year 2022 marks 14 years of my scientific and research career: 14 years of R&D

This year 2022 marks 14 years of my scientific and research career mainly in Ghana. In 2015 I had the privilege of participating in a workshop and a conference organized by the Grand Challenges Canada in Nairobi Kenya to begin the process of implementing the concept of integrated innovation which seeks to transform the results of scientific research into a technological enterprise.

During my graduate training and in the first 7 years of my career I didn't factor into my plans this enterprise development exercise. So it came as an interesting shift in my goals to suddenly begin to think like a businessman. In the USA and other places University Professors creating companies out of their research is quite common. Here in Ghana, the resources for conducting research alone is impossible to come by so how am I supposed to advance into enterprise development?

I have devoted much of the second seven years of my career to this impossible dream. This second seven comes to a close this year 2022. This piece is therefore a tribute to a goal not yet realized despite best efforts. I have competed in 6 funding programs and none has yet been successful. I therefore have a decision to make as I open the third seven years of my career.

The research work so far points to the possibility of a Biotech enterprise in Ghana and I have a small but determined team willing to stick to the mission. We are also actively engaging with investors for the capital to take off and build a unique company that produces fine chemicals and pharmaceutical ingredients. And we will do this by exploiting fungal fermentation at scale.

Mindful of the importance of pharmaceutical ingredients I sought the mentorship of Paul A. Lartey, Ph.D. and Dr. Alexandra E. Graham, MBA of LaGray Chemical Company in Ghana in 2012 on. The interactions have been an eye opening journey and now that mission continue onto enterprise development and I will strive for this goal

Many of the substances we study shine brightly and I hope the light they produce will soon become the light in our path.

MultiOMICS approach to Chemical Systems Biology Strategy

On the question of data integration in multiomics project to derive mechanistic insights:

My take is that there is the need to first pursue concept integration ahead of data integration. As many data elements exhibit stochasticity over time and some conditions, data integration suffers.

At conceptual or mechanistic level, a smaller set key data points are relevant which are also not that stochastic.

These key data elements are those which are also classified as "necessary and sufficient" in classical biology studies.

This approach one of the efficient ways to deal with multiomics datasets, which is expected to be less prone to data artefacts.

That said, data integration should be iterative at best, starting with the conceptual and then add more data points that share the features of the key data points and on and on while controlling for stochastic and longitudinal effects.

However, in situations where such conceptual frameworks do not exist, data clustering can offer the way forward. The clusters can be studied and organized in a hierarchical order to suggest the first draft of mechanisms and then iterate as earlier stated.

Questions?

Contact pakar.laboratories@gmail.com to get more information on the project

Join Us

Wood-decaying fungi found in Ghana: A rich source of new anti-infective compounds

Background: Discovery of bioactive natural products are instrumental for development of novel antibiotics. The discovery and development of natural products such as penicillin represented a major milestone in the treatment of bacterial infections. Currently, many antibiotics have lost their relevance in clinics due to the emergence of drug-resistant microbial pathogens. Hence, there is the need for continuous search of new compounds endowed with potent antimicrobial activity.

Methods: In this study, wood-decaying fungi (WDF) from Ghana were explored for their potential as sources of novel antimicrobial compounds with intent of expanding the effort into a drug discovery programme in the near future. Extracts from cultures of 54 morphologically distinct WDF isolates were analyzed for the presence of antimicrobial agents.

Results: The extracts from 40 out of the 54 WDF isolates exhibited significant antimicrobial activity against either Staphylococcus aureus, Escherichia coli or Candida albicans. Fractionation of these bioactive extracts, followed by bioassay of the organic fractions obtained, indicate that extracts exhibiting antimicrobial activity against more than one of the three test organisms could be attributed to the presence of different bioactive compounds. Analysis of the composition of the extracts revealed that terpenes were predominant.

Conclusions: This study suggests that a significant proportion of WDF in Ghana produce antimicrobial compounds which could be potential sources of novel anti-infective agents and support the plans of developing a drug discovery programme in Ghana based on the fermentation of WDF.

DNA double-strand break formation and repair as targets for novel antibiotic combination chemotherapy

Executive summary

This article discusses a strategy for the development of robust chemotherapy against antimicrobial-resistant infections by:

Providing insights into the possibility of exploiting the bacterial double-strand break (DSB) repair pathways as targets.

Exploring crucial synergistic combinations between DSB-inducing compounds and inhibitors of DSB repair.

Examining the effectiveness of this new therapeutic regimen in combating antimicrobial-resistant infections.

The proposed chemotherapeutic strategy is discussed as a perspective, highlighting the possibility of utilizing bacterial DSB repair pathways as targets for the discovery and development of novel antibiotics.

Characterization of Two New Multidrug-Resistant Strains of Mycobacterium smegmatis_Tools for Routine In Vitro Screening of Novel Anti-Mycobacterial Agents

Mycobacterium tuberculosis is a pathogen of global public health concern. This threat is exacerbated by the emergence of multidrug-resistant and extremely-drug-resistant strains of the pathogen. We have obtained two distinct clones of multidrug-resistant Mycobacterium smegmatis after gradual exposure of Mycobacterium smegmatis mc2 155 to increasing concentrations of erythromycin. The resulting resistant strains of Mycobacterium smegmatis exhibited robust viability in the presence of high concentrations of erythromycin and were found to be resistant to a wide range of other antimicrobials. They also displayed a unique growth phenotype in comparison to the parental drug-susceptible Mycobacterium smegmatis mc2 155, and a distinct colony morphology in the presence of cholesterol. We propose that these two multidrug-resistant clones of Mycobacterium smegmatis could be used as model organisms at the inceptive phase of routine in vitro screening of novel antimicrobial agents targeted against multidrug-resistant Mycobacterial tuberculosis.

Transcriptomic analysis of marine endophytic fungi extract identifies highly enriched anti-fungal fractions targeting cancer pathways in HepG2 cell lines

Background: Marine endophytic fungi (MEF) are good sources of structurally unique and biologically active secondary metabolites. In this study, ethyl acetate extracts from marine endophytic fungal cultures were tested for their antifungal activity and anticancer properties

against C. albicans and the human liver cancer cell line HepG2, respectively. The highly enriched fractions were also analyzed by high performance liquid chromatography coupled with high resolution mass spectrometry (HPLCHRMS) and their effect on the HepG2 cells was assessed via transcriptomics and with a proliferation assay.

Results: We demonstrated that the fractions could reduce proliferation in HepG2 cells. The detailed transcriptome analysis revealed regulation of several cancer- and metabolism-related pathways and gene ontologies. The downregulated pathways included, cell cycle, p53 signaling, DNA replication, sphingolipid metabolism and drug metabolism by cytochrome P450. The upregulated pathways included HIF-1 signaling, focal adhesion, necroptosis and transcriptional mis-regulation of cancer. Furthermore, a protein interaction network was constructed based on the 26 proteins distinguishing the three treatment conditions from the untreated cells. This network was composed of central functional components associated with metabolism and cancer such as TNF, MAPK, TRIM21 and one component contained APP.

Conclusions: The purified fractions from MEF investigated in this study showed antifungal activity against C. albicans and S. cerevisiae alone or both and reduced proliferation of the human liver cancer cell line HepG2 implicating regulation of several cancer- and metabolism-related pathways. The data from this study could be instrumental in identifying new pathways associated with liver cancer anti-proliferative processes which can be used for the development of novel antifungal and anti-cancer drugs.

Page updated

Google Sites

Report abuse