T cell response is produced by temporally-dynamic activities of polyclonal T cells (i.e. heterogenous T cells in natural settings, each of which has unique T cell receptor [TCR] structures).
We thus developed Tocky to investigate temporally dynamic mechanisms underlying T cell activation, differentiation and response.
1. Timer-of-Cell-Kinetics-and-Activity (Tocky とき).
Tocky is a single-cell method using Fluorescent Timer protein to analyse temporally-dynamic activities of individual cells in vivo.
Read our paper which reported Tocky, which established the use of TCR-Tocky (Nr4a3-Tocky) to analyse:
(1) temporal sequence of Treg development
(2) tonic TCR signalling in Treg and memory-phenotype T-cells; and
(3) antigen-reactive T-cells in neural inflammation.
2. Canonical Correspondence Analysis (CCA) for multidimensional analysis of multiple transcriptomes.
We used CCA to develop our Tocky for TCR signalling (Nr4a3-Tocky)
A single cell method using CCA (Single Cell CCA, SC4A)
3. We proposed a new model for Foxp3-mediated T cell regulation from a dynamic perspective (Ono & Tanaka, 2016), which was addressed by Tocky (Bending et al, 2018).
