Fig 1: Mice utilizing Red Huts. This two-story hut can be manipulated by mice in the cage. It has 2 entrance holes on the first story and an entrance on the second level.
< When deciding on the enrichment to reflect natural nesting and hiding behaviors we decided on these plastic red huts. These huts give the mice multiple opportunities to nest in the lower levels or just simply use it as a way to climb and interact on. We hoped that cages with higher mice densities (n=5) would simply have a way to escape the barberer.
Fig 2: Mouse manipulating wood block. Wood blocks and a fluffy nestlet are all present in this treatment group. They remain in the cage for long periods of time.
< Wood Blocks were selected to reflect gnawing behaviors mice naturally exhibit. We know that rodent teeth never quit growing and were curious to see if giving them something to naturally chew on would limit their barbering behavior.
Fig 3: Mice in a cage (n=5 max) after a cage change-out. Mice are given 1 scoop of seeds at cage change and a new fluffy nestlet.
< Irradiated Sunflower Seeds are distributed in a cage. This enrichment was selected to reflect foraging behaviors that mice naturally exhibit. This novel activity was presented to them once every 2 weeks and we hoped the activity would limit their barbering behavior.
< While not a direct enrichment, we were interested as a program to switch to a paper/cellulose based bedding (Biofresh Performance Bedding) for other benefits like higher absorbency and ammonia control. We decided to see if our study could capture any differences from our control group in our barber occurrences, as this was an environmental change.
Fig 5: Dam utilizing fluffy nestlet to build nest after cage change-out. Barbering occuring within this cage present on dam's side.
< Our control group received corn cob bedding and a nestlet only. For this group while the purpose was our control it was interesting to see whether occurrence would go up or down throughout the study.
Fig 6: Mice would be observed to pin each other down and groom obsessively over areas. Mice were also discovered to be balding or dispersedly losing hair. The colony is SPF for all known opportunistic bacterial and parasitic agents including Demodex sp. and is tested monthly using PCR on rack exhaust air or polled dirty bedding.
Barbering was up to ~25% in our colony prior to study start (n=796).
< An example of barbering behavior observed in our NSG colony.