Molecular biology and Enzymology continue to make major strides and are rapidly advancing due to their major role in biomedical and pharmacological applications. Some information can be obtained at home even by being without any expensive resources, whereas many skills have to be learnt at the bench. Molecular biology is a subject, in which there is an inherent lag in teaching and learning of the concepts due to lack of exposure and opportunity to learn by hands-on experience. Faculty need to update and reorient themselves to meet the rapidly changing professional and scientific needs of the stakeholders. This workshop focuses on teaching several basic concepts, including recombinant DNA, plasmids, restriction enzymes, primer design, enzyme assay methods, inhibition kinetics for enzymes as drug targets and provides hands on training in the techniques practiced in molecular biology and enzymology. Both theoretical knowledge and hands-on training of skills by experts in the field will provide an in-depth understanding of basic methods in molecular biology and enzymology.