IV. Acute slice preparation tips

SCJ ver. acute slice cutting solution

pH 7.3~7.4

1. This solution should be used for only cutting acute slices. After cutting, the incubation and storage of slices should be performed in normal recording solution.

2. This high Mg2+ cutting solution is working well for young rat or mice brain cutting (up to 4 weeks). Some recipes containing sucrose may be better for older animals (Dr. Bunano lab protocol, Kwon et al., 2008, PNAS).

3. Sagittal section is generally used for cell body recording, and semi sagittal section for dendritic patches.

4. 350 um thickness is proper for EPSP and EPSC recording.

5. SCJ ver. 37 degree, 30 min incubation after cutting was good for EPSC recording.