8 , Fatimah Abdullah 9 , Amr Alnagar 10, Nashwa ElTaweel 11, Majed Alharthi 12 , Ali Mohsin 13, Ana Ordóñez-Cruickshank 7 , Bianca Toniolo 14, Tâmela Grafolin 14 , Thit Thit Aye 15 , Yong Zhin Goh 7 , Ehsan Akram Deghidy 16, Siti Bahri 17, Jarntrah Sappayabanphot 18 , Yasir Ahmed Mohammed Elhadi 19 , Salma Mohammed 20, Ahmed Nour El-Deen 21, Ismail Ismail 22 , Samar Abd ElHafeez 3 , Iffat Elbarazi 23 , Basema Saddik 24 , Ziad El-Khatib 25 , Hiba Mohsin 26 and Ahmed Kamal 27 1 Department of Instructional Technology and Learning Sciences, Utah State University, Logan, UT 84321, USA; ramy.shaaban@usu.edu 2 Tropical Health Department, High Institute of Public Health, Alexandria University, Alexandria 21561, Egypt; ramy_ghazy@alexu.edu.eg 3 Epidemiology Department, High Institute of Public Health, A.); samarabdelhafeez.epid@gmail.com (S.A.E.) 4 Department of Communications Media, Indiana University of Pennsylvania, Indiana, PA 15705, USA; n.s.ali@iup.edu (N.A.); Egypt; ronyibrahim13@hotmail.com 7 Institute of Tropical Medicine and International Health, Charité—Universitätsmedizin Berlin, 13353 Berlin, Germany; bonny-dowd.mckinna@charite.de (B.M.); ana-magdalena.ordonez-cruickshank@charite.de (A.O.-C.); yong-zhin.goh@charite.de (Y.Z.G.) 8 Training and Biostatistics Administration, Ministry of Health and Population, Alexandria 21561, Egypt; hiph.amiraelzorkany@alexu.edu.eg 9 Internal Medicine Department, Alexandria University, Alexandria 21526, Egypt; fatemaalabed94@gmail.com 10 General Surgery Department, Faculty of Medicine, Alexandria University, Alexandria 21561, Egypt; amr.alnagar@alexmed.edu.eg 11 University Hospital of Coventry and Warwickshire, Coventry CV2 2DX, UK; dr_nashwa.anwar@hotmail.com 12 Faculty of Communication and Media, King Abdulaziz University, Jeddah 22254, Saudi Arabia; malharthi5@kau.edu.sa 13 Biomedical Engineering Department, Collage of Engineering, Wraith Al-Anbiyaa University, Karbala 56001, Iraq; alikareem.mohsin@gmail.com 14 LabCom Research Unit, University of Beira Interior, Foundation for Science and Technology, 6201-001 Covilha, Portugal; bianca.toniolo@ubi.pt (B.T.); tamela.grafolin@ubi.pt (T.G.) 15 Heidelberg Institute of Global Health, Heidelberg University, 69120 Heidelberg, Germany; thitthit.aye@uni-heidelberg.de 16 Department of Biomedical Informatics and Medical Statistics, Medical Research Institute, Alexandria University, Alexandria 21526, Egypt; drehsan.deghidy@yahoo.com 17 Pharmacy Department, Rompin Hospital, Ministry of Health Malaysia, 62590 Putrajaya, Malaysia; siti.sbahri@gmail.com 18 Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand; jarntrah@tropmedres.ac 19 Department of Public Health, Medical Research Office, Sudanese Medical Research Association, Khartoum 11111, Sudan; hiph.yelhadi@alexu.edu.eg 20 Department of Women’s and Children’s Health, Uppsala University, 75236 Uppsala, Sweden; salmaelmukashfieltahir.mohammed.0806@student.uu.se 21 Department of Physiology, Faculty of Medicine, Al-Azhar University, Assiut 71524, Egypt; drnoor83@hotmail.com 22 Department of Neurology, Ibn Sina Hospital, Safat, Kuwait City 13115, Kuwait; dr.ismail.ibrahim2012@gmail.com 23 Institute of Public Health, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain 15551, United Arab Emirates; ielbarazi@uaeu.ac.ae 24 College of Medicine, University of Sharjah, Sharjah 27272, United Arab Emirates;. J. Environ. Res. Public Health 2022, 19, 5737. Environ. Res. Public Health 2022, 19, 5737 2 of 14 25 Department of Global Public Health, Karolinska Institutet, 17176 Stockholm, Sweden; ziad.khatib@gmail.com 26 College of Pharmacy, Al-Zahraa University for Women, Karbala 56001, Iraq; hiba.akram@alzahraa.edu.iq 27 Hepatology Unit, Internal Medicine Department, Faculty of Medicine, Alexandria University, Alexandria 21131, Egypt; ahmed.kamal@alexmed.edu.eg * Correspondence: hiph.felsherif@alexu.edu.eg; Tel.: +20-12-7916-6080 † These authors contributed equally to this work. Abstract: Vaccine hesitancy (VH) is defined as a delayed in acceptance or refusal of vaccines despite availability of vaccination services. This multinational study examined user interaction with social media about COVID-19 vaccination. The study analyzed social media comments in 24 countries from five continents. In total, 5856 responses were analyzed; 83.5% of comments were from Facebook, while 16.5% were from Twitter. In Facebook, the overall vaccine acceptance was 40.3%; the lowest acceptance rates were evident in Jordan (8.5%), Oman (15.0%), Senegal (20.0%) and Morocco (20.7%) and the continental acceptance rate was the lowest in North America 22.6%. In Twitter, the overall acceptance rate was (41.5%); the lowest acceptance rate was found in Oman (14.3%), followed by USA (20.5%), and UK (23.3%) and the continental acceptance rate was the lowest in North America (20.5%), and Europe (29.7%). The differences in vaccine acceptance across countries and