Metabolomics and Natural Products Screening
Fungal Metabologenomics
Fungal Metabologenomics
We probed fungi for anticancer, antifungal, and antiparasitic activity through high throughput bioactivity screening, metabolomics based bioactive molecule identification and genomics analysis to identify the biosynthetic gene clusters that are making those bioactive molecules using a metabologenomics platform.
We probed fungi for anticancer, antifungal, and antiparasitic activity through high throughput bioactivity screening, metabolomics based bioactive molecule identification and genomics analysis to identify the biosynthetic gene clusters that are making those bioactive molecules using a metabologenomics platform.
Workflow for integration of metabolomics and genomics to identify bioactive fungal secondary metabolites and their biosynthetic gene clusters.
I grew 276 fungi strains under various growth conditions, extracted their secondary metabolites, and ran LC-MS/MS for metabologenomics and bioactivity screening.
I grew 276 fungi strains under various growth conditions, extracted their secondary metabolites, and ran LC-MS/MS for metabologenomics and bioactivity screening.
Bacterial Metabolomics
Bacterial Metabolomics
We used targeted LC-MS/MS methods to study the effect of drugs on cell wall and DNA biosynthesis pathway in bacteria.
Cytoplasmic intermediates and the enzymes involved in S. aureus cell wall biosynthesis pathway showing stepwise formation of functional units leading to peptidoglycan formation.
(Ref.: Vemula et al. Biochimie 2016)
