Publications

Comparison of 3D cellular imaging techniques based on scanned electron probes: serial block face SEM vs. axial bright-field STEM tomography

McBride EL, Rao A, Zhang G, Hoyne JD, Calco GN, Kuo BC, He Q, Prince AA, Pokrovskaya ID, Storrie B, Sousa AA, Aronova MA, Leapman RD. Journal of Structural Biology, 202, 216-228 (2018).

Identification of PSD-95 in the Postsynaptic Density Using MiniSOG and EM Tomography

Chen X, Winters C, Crocker V, Lazarou M, Sousa AA, Leapman RD, Reese TS. Frontiers in Neuroanatomy. 12, 107 (2018).

Physicochemical characterization of ferumoxytol, heparin and protamine nanocomplexes for improved magnetic labeling of stem cells

Bryant LH, Kim SJ, Hobson M, Milo B, Kovacs ZI, JiKiria N, Lewis BK, Aronova MA, Sousa AA, Zhang G, Leapman RD, Frank JA. Nanomedicine: Nanotechnology, Biology and Medicine, 13, 503-513 (2017).

PAR1 activation induces rapid changes in glutamate uptake and astrocyte morphology

Sweeney AM, Fleming KE, McCauley JP, Rodriguez MF, Martin ET, Sousa AA, Leapman RD, Scimemi A. Scientific Reports, 7 (2017).

PSD-95 family MAGUKs are essential for anchoring AMPA and NMDA receptor complexes at the postsynaptic density

Chen X, Levy JM, Hou A, Winters C, Azzam R, Sousa AA, Leapman RD, Nicol RA, Reese TS. Proceedings of the National Academy of Sciences USA, 112, 6983-6992 (2015).

Passive Diffusion as a Mechanism Underlying Ribbon Synapse Vesicle Release and Resupply

Graydon CW, Zhang J, Oesch NW, Sousa AA, Leapman RD, Diamond JS. Journal of Neuroscience, 34, 8948-8962 (2014).

Nanoscale imaging of protein molecules at the postsynaptic density

Chen X, Winters C, Azzam R, Sousa AA, Leapman RD, Reese TS. In: Valentin Nägerl; Antoine Triller (Eds.). Nanoscale Imaging of Synapses. Neuromethods (Springer) v. 84 (2014).

Cryo-electron tomography of the magnetotactic vibrio Candidatus Magnetovibrio blakemorei

Abreu F, Sousa AA, Aronova AA, Leapman RD, Andrade L, Kachar B, Bazylinski D, Lins U. Journal of Structural Biology, 181, 162-168 (2013).

Synthesis, characterization and direct intracellular imaging of ultrasmall and uniform glutathione-coated gold nanoparticles

Sousa AA, Morgan JT, Brown PH, Adams A, Zhang G, AckersonCJ, Kruhlak MJ, Leapman RD. Small, 8,2277-2286 (2012).

Development and application of STEM for the biological sciences [Review]

Sousa AA, Leapman RD. Ultramicroscopy, 123, 38-49 (2012). 

Nanoimaging: Methods and Protocols, 1 ed.

Ed: Alioscka A. Sousa & Michael J Kruhlak. Methods in Molecular Biology (Springer/Humana Press), 2012.

Mass mapping of amyloid fibrils in the electron microscope using STEM imaging

Sousa AA, Leapman RD. In: Alioscka A. Sousa; Michael J. Kruhlak (Eds.). Nanoimaging: Methods and Protocols. 1ed. Methods in Molecular Biology (Springer/Humana Press), v. 950, p. 195-207 (2012).

Aggregation and fibril morphology of the artic mutation of Alzheimer’s Abeta peptide by CD, TEM, STEM and in situ AFM

Norlin N, Hellberg M, Filippov A, Sousa AA, Grobner G, Leapman RD, Almqvist N, Antzutkin ON. Journal of Structural Biology, 180, 174-189 (2012).

Direct visualization of CamKII at postsynaptic densities by EM tomography

Fera A, Dosemeci A, Sousa AA, Yang C, Leapman RD, Reese TS.  Journal of Comparative Neurology, 520, 4218-4225 (2012).

Dual axis electron tomography of biological specimens: Extending the limits of specimen thickness with bright-field STEM imaging

Sousa AA, Azari AA, Zhang G, Leapman RD. Journal of Structural Biology, 174, 107-114 (2011).

PSD-95 is required to sustain the molecular organization of the postsynaptic density

Chen X, Nelson CD, Li X, Winters CA, Azzam R, Sousa AA, Leapman RD, Gainer H, Sheng M, Reese TS. Journal of Neuroscience, 31, 6329-6338 (2011).

Effect of the charge state on the nuclear magnetic resonance of monodisperse Au25[S(CH2)2Ph]18z clusters

Venzo A, Antonello S, Gasco JA, Guryanov I, Leapman RD, Perera NV, Sousa AA, Zamuner M, Zanella A, Maran F.  Analytical Chemistry, 83, 6355-6362 (2011).

Combining portable Raman probes with nanotubes for theranostic applications

Bhirde AA, Liu G, Jin A, Iglesias R, Sousa AA, Leapman RD, Gutkind JS, Lee S, Chen X. Theranostics, 1, 310-321 (2011).

EELS characterization of radiolytic products in frozen samples 

Aronova MA, Sousa AA, Leapman RD. Micron 42, 252-256 (2011).

Distribution and clearance of PEG-single-walled carbon nanotube cancer drug delivery vehicles in mice

Bhirde AA, Patel S, Sousa AA, Patel V, Molinolo AA, Ji Y, Leapman RD, Gutkind JS, Rusling JF. Nanomedicine, 5, 1535-1546 (2010). 

Limitations of beam damage in electron spectroscopic tomography of embedded cells

Aronova MA, Sousa AA, Zhang G, Leapman RD. Journal of Microscopy, 239, 223-232 (2010).

Nanoscale 3D cellular imaging by axial scanning transmission electron tomography

Hohmann-Marriott MF, Sousa AA, Glushakova S, Azari A, Zhang G, Zimmerberg J, Leapman RD. Nature Methods, 6, 729-731 (2009).

Monte Carlo electron-trajectory simulations in bright-field and dark-field STEM: implications for tomography of thick biological specimens

Sousa AA, Hohmann-Marriott MF, Zhang G, Leapman RD. Ultramicroscopy, 109, 213-221 (2009).

Determining molecular mass distributions and compositions of functionalized dendrimer nanoparticles 

Sousa AA, Aronova MA, Wu H, Sarin H, Griffiths GL, Leapman RD. Nanomedicine, 4, 391-399 (2009). 

Physiologic upper limit of pore size in the blood-tumor barrier of malignant solid tumors

Sarin H, Kanevsky AS, Wu H, Sousa AA, Wilson CM, Aronova MA, Griffiths GL, Leapman RD, Vo HQ. Journal of Translational Medicine, 7, 51 (2009).

Targeted killing of cancer cells in vivo and in vitro with EGF-directed carbon nanotube-based drugs

Bhirde AA, Patel V, Gavard J, Zhang G, Sousa AA, Masedunskas A, Leapman RD, Weigert R, Gutkind JS, Rusling JF. ACS Nano, 3, 307-316 (2009).

Selective adsorption of surface-modified ferritin on a phase-separated polymer blend

Sengonul M, Sousa A, Libera M. Colloids and Surfaces B - Biointerfaces, 73, 152-155 (2009).

Effective transvascular delivery of nanoparticles across the blood-brain tumor barrier into malignant glioma cells 

Sarin H, Kanevsky AS, Wu H, Brimacombe KR, Fung SH, Sousa AA, Auh S, Wilson CM, Aronova MA, Leapman RD, Griffiths GL, Sharma K, Hall MD. Journal of Translational Medicine, 6, 80 (2008). 

Determination of quantitative distributions of heavy-metal stain in biological specimens by annular dark-field STEM

Sousa AA, Hohmann-Marriott MF, Aronova MA, Zhang G, Leapman RD. Journal of Structural Biology, 162, 14-28 (2008).

Nanoscale composition of biphasic polymer nanocolloids in aqueous suspension

Kim G, Sousa AA, Meyers D, Libera M. Microscopy and Microanalysis, 14, 459-468 (2008).

On the feasibility of visualizing ultrasmall immunogold labels in biological specimens by STEM tomography

Sousa AA, Aronova MA, Kim YC, Dorward LM, Zhang G, Leapman RD. Journal of Structural Biology, 159, 507-522 (2007).

Three-dimensional elemental mapping of phosphorus by quantitative electron spectroscopic tomography

Aronova MA, Kim YC, Harmom R, Sousa AA, Zhang G, Leapman RD. Journal of Structural Biology, 160, 35-48 (2007).

Quantitative STEM mass measurement of biological macromolecules in a 300 kV TEM

Sousa AA, Leapman RD. Journal of Microscopy, 228, 25-33 (2007).

Selective protein adsorption on a phase-separated solvent-cast polymer blend

Sousa A, Sengonul M, Latour R, Kohn J, Libera M. Langmuir, 22, 6286-6292 (2006).

Diffuse polymer interfaces in lobed nanoemulsions preserved in aqueous media

Kim G, Sousa A, Meyers D, Shope M, Libera M. Journal of the American Chemical Society, 128, 6570-6571 (2006). 

Nanoscale morphological changes during hydrolytic degradation and erosion of a bioresorbable polymer

Sousa A, Schut J, Kohn J, Libera M. Macromolecules, 39, 7306-7312 (2006). 

Water mapping in hydrated soft materials

Sousa A, Aitouchen A, Libera M. Ultramicroscopy, 106, 130-145 (2006).