CV Erwan GUEGUEN

CURRENT POSITION

Assistant-Professor (Maître de Conférences hors-classe), HDR, at Claude Bernard University Lyon I since September 1, 2012.

Head of microbial genetics teaching team (13 faculty members) since September 2020. Head of the Life Sciences Bachelor's Microbiology program since 2022

Professional Contact: Erwan GUEGUEN, PhD MAP Laboratory Claude Bernard University Lyon 1 10 rue Raphaël Dubois Lwoff building 69622 Villeurbanne Cedex France

Profile: Molecular bacteriology and bacterial genetics. Affiliated with the "Microbiology, Adaptation and Pathogenicity" laboratory CNRS UMR5240

erwan.gueguen@univ-lyon1 http://map.univ-lyon1.fr

EDUCATION AND DEGREES

• 2021. Habilitation to Direct Research. Claude Bernard University, Lyon, France.

• 2002-2006. PhD in Genetics and Microbiology, 2006. Paul Sabatier University, Toulouse, France.

ACADEMIC CAREER

• Since 2015: Assistant Professor in the "Membrane Traffic and Bacterial Signaling (MTSB)" team, CNRS-MAP, Villeurbanne (team leaders Agnès Rodrigue and Vladimir Shevchik). Study of pathogenicity mechanisms in phytopathogenic bacteria of the genus Dickeya.

• 2012 to 2015: Associate Professor in the "bacteria and metals" team, CNRS-MAP, Villeurbanne (team leader Agnès Rodrigue). Study of bacterial resistance mechanisms to heavy metals.

• 2010-2012: Post-doctoral researcher in Eric Cascales' team "Assembly of trans-membrane systems", CNRS-LISM, Marseille. Characterization of type VI secretion systems in Yersinia pseudotuberculosis and Citrobacter rodentium.

• 2007-2010: Post-doctoral researcher. Dr. Andrew Darwin's Laboratory, New York University, USA. Study of the Phage Shock Protein system in the pathogenic bacterium Yersinia enterocolitica.

• 2002-2006: PhD student. Chandler Team, CNRS-LMGM, Toulouse. Study of transposition of the bacterial mobile element IS911.

RESEARCH ACTIVITIES

PUBLICATIONS

The names of PhD students I supervised are highlighted.

1. Lorang C, Canto P-Y, Gueguen E, Pédron J, Barny M-A. Pectobacterium versatile β-Lactamase Contributes to Soft Rot Pectobacteriaceae (SRP) Community Diversity During Potato Infection. Environmental Microbiology Reports. 2025;17: e70111.

2. Baffert Y, Fraikin N, Makhloufi Y, Baltenneck J, Val M-E, Dedieu-Berne A, Degosserie J, Iorga BI, Bogaerts P, Gueguen E, Lesterlin C, Bigot S. Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli. Nat Commun. 2025;16: 7734.

3. Royet K, Kergoat L, Lutz S, Oriol C, Parisot N, Schori C, et al. High-Throughput Tn-Seq Screens Identify Both Known and Novel KT2440 Genes Involved in Metal Tolerance. Environmental Microbiology. 2025;27: e70095. ($ Corresponding author)

4. Dubois Q, Brual T, Oriol C, Mandin P, Condemine G, Gueguen E$ . *Function and mechanism of action of the small regulatory RNA ArcZ in Enterobacterales.* RNA. 2024 Jun 5:rna.080010.124. doi: 10.1261/rna.080010.124. PMID: 38839110. ($ Corresponding author)

5. Brual T, Effantin G, Baltenneck J, Attaiech L, Grosbois C, Royer M, Cigna J, Faure D, Hugouvieux-Cotte-Pattat N, Gueguen E$. A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria. PLoS Genet. 2023 Apr 27;19(4):e1010725. doi: 10.1371/journal.pgen.1010725. ($ Corresponding author)

6. Robic K, Munier E, Effantin G, Lachat J, Naquin D, Gueguen E, Faure D. 2023. Dissimilar gene repertoires of Dickeya solani involved in the colonization of lesions and roots of Solanum tuberosum. Front Plant Sci 14:1154110.

7. Job V, Gomez-Valero L, Renier A, Rusniok C, Bouillot S, Chenal-Francisque V, Gueguen E, Adrait A, Robert-Genthon M, Jeannot K, Panchev P, Elsen S, Fauvarque MO, Couté Y, Buchrieser C, Attrée I. Genomic erosion and horizontal gene transfer shape functional differences of the ExlA toxin in Pseudomonas spp. iScience. 2022 Jun 14;25(7):104596. 

8. Morinière L, Mirabel L, Gueguen E, Bertolla F. A Comprehensive Overview of the Genes and Functions Required for Lettuce Infection by the Hemibiotrophic Phytopathogen Xanthomonas hortorum pv. vitians. mSystems. 2022 Mar 21:e0129021. doi: 10.1128/msystems.01290-21

9. Hugouvieux-Cotte-Pattat N, Royer M, Gueguen E, Le Guen P, Süssmuth RD, Reverchon S, Cociancich S. Specificity and genetic polymorphism in the Vfm quorum sensing system of plant pathogenic bacteria of the genus Dickeya. Environ Microbiol. 2022 Jan 10. doi: 10.1111/1462-2920.15889.

10. Morinière L, Lecomte S, Gueguen E, Bertolla F. In-depth exploration of Xanthomonas hortorum pv. vitians essential genome in vitro using transposon insertion sequencing and comparative genomics discriminated between core and contextual essential genes. Microb Genom. 2021 Mar 24. doi: 10.1099/mgen.0.000546

11. Reuter A, Hilpert C, Berne AD, Lematre S, Gueguen E, Launay G, Bigot S, Lesterlin C. Targeted-Antibacterial-Plasmids (TAPs) combining conjugation and CRISPR/Cas systems achieve strain-specific antibacterial activity. Nucleic Acids Res. 2021 Apr 6;49(6):3584-3598. doi: 10.1093/nar/gkab126.

12. Hugouvieux‐Cotte‐Pattat N, Condemine G, Gueguen, E and Shevchik VE. (2020). Dickeya Plant Pathogens. In eLS, John Wiley & Sons, Ltd (Ed.).

13. Royet K, Parisot N, Rodrigue A, Condemine G*, Gueguen E$*. Identification by Tn-seq of Dickeya dadantii genes required for survival in chicory plants. Mol Plant Pathol. 2019 Feb;20(2):287-306. doi: 10.1111/mpp.12754. Epub 2018 Nov 15. PubMed PMID: 30267562. (Co-last authorship $ Corresponding author)

14. Rome K, Borde C, Taher R, Cayron J, Lesterlin C, Gueguen E, De Rosny E, Rodrigue A. The Two-Component System ZraPSR Is a Novel ESR that Contributes to Intrinsic Antibiotic Tolerance in Escherichia coli. J Mol Biol. 2018 Dec 7;430(24):4971-4985. doi: 10.1016/j.jmb.2018.10.021. Epub 2018 Oct 30. PubMed PMID: 30389436.

15. Cayron J, Prudent E, Escoffier C, Gueguen E, Mandrand-Berthelot MA, Pignol D, Garcia D, Rodrigue A. Pushing the limits of nickel detection to nanomolar range using a set of engineered bioluminescent Escherichia coli. Environ Sci Pollut Res Int. 2017 Jan;24(1):4-14. doi:10.1007/s11356-015-5580-6. Epub 2015 Oct 24. PubMed PMID: 26498802.

16. Petit-Härtlein I, Rome K, de Rosny E, Molton F, Duboc C, Gueguen E, Rodrigue A, Covès J. Biophysical and physiological characterization of ZraP from Escherichia coli, the periplasmic accessory protein of the atypical ZraSR two-component system. Biochem J. 2015 Dec 1;472(2):205-16. doi:10.1042/BJ20150827. Epub 2015 Oct 5. PubMed PMID: 26438879.

17. Gueguen E$, Wills NM, Atkins JF, Cascales E$. Transcriptional frameshifting rescues Citrobacter rodentium type VI secretion by the production of two length variants from the prematurely interrupted tssM gene. PLoS Genet. 2014 Dec 4;10(12):e1004869. doi: 10.1371/journal.pgen.1004869. eCollection 2014 Dec. PubMed PMID: 25474156. ($ Co-corresponding author)

18. Blériot C, Gault M, Gueguen E, Arnoux P, Pignol D, Mandrand-Berthelot MA,Rodrigue A. Cu binding by the Escherichia coli metal-efflux accessory protein RcnB. Metallomics. 2014 Aug;6(8):1400-9. doi: 10.1039/c4mt00036f. PubMed PMID: 24658095.

19. Gueguen E$, Durand E, Zhang XY, d'Amalric Q, Journet L, Cascales E$. Expression of a Yersinia pseudotuberculosis Type VI Secretion System Is Responsive to Envelope Stresses through the OmpR Transcriptional Activator. PLoS One. 2013 Jun 19;8(6):e66615. doi:0.1371/journal.pone.0066615. Print 2013. PubMed PMID: 23840509. ($ Co-corresponding author)

20. Gueguen E$, Cascales E. Promoter swapping unveils the role of the Citrobacter rodentium CTS1 type VI secretion system in interbacterial competition. Appl Environ Microbiol. 2013 Jan;79(1):32-8. doi: 10.1128/AEM.02504-12. Epub 2012 Oct 12. PubMed PMID: 23064344. ($ Corresponding author)

21. Gueguen E, Flores-Kim J, Darwin AJ. The Yersinia enterocolitica phage shock proteins B and C can form homodimers and heterodimers in vivo with the possibility of close association between multiple domains. J Bacteriol. 2011 Oct;193(20):5747-58. doi: 10.1128/JB.05080-11. Epub 2011 Aug 19. PubMed PMID: 21856846.

22. Yamaguchi S, Gueguen E, Horstman NK, Darwin AJ. Membrane association of PspA depends on activation of the phage-shock-protein response in Yersinia enterocolitica. Mol Microbiol. 2010 Oct;78(2):429-43. PubMed PMID: 20979344.

23. Bernard CS, Brunet YR, Gueguen E, Cascales E. Nooks and crannies in type VI secretion regulation. J Bacteriol. 2010 Aug;192(15):3850-60. doi:10.1128/JB.00370-10. Epub 2010 May 28. Review. PubMed PMID: 20511495.

24. Gueguen E, Savitzky DC, Darwin AJ. Analysis of the Yersinia enterocolitica PspBC proteins defines functional domains, essential amino acids and new roles within the phage-shock-protein response. Mol Microbiol. 2009 Nov;74(3):619-33. doi: 10.1111/j.1365-2958.2009.06885.x. Epub 2009 Sep 22. PubMed PMID: 19775245.

25. Gueguen E, Rousseau P, Duval-Valentin G, Chandler M. Truncated forms of IS911 transposase downregulate transposition. Mol Microbiol. 2006 Nov;62(4):1102-16. PubMed PMID: 17078817.

26. Gueguen E, Rousseau P, Duval-Valentin G, Chandler M. The transpososome: control of transposition at the level of catalysis. Trends Microbiol. 2005 Nov;13(11):543-9. Epub 2005 Sep 21. Review. PubMed PMID: 16181782.

27. Rousseau P, Gueguen E, Duval-Valentin G, Chandler M. The helix-turn-helix motif of bacterial insertion sequence IS911 transposase is required for DNA binding. Nucleic Acids Res. 2004 Feb 23;32(4):1335-44. Print 2004. PubMed PMID: 14981152.

TEACHING

Assistant Professor in Microbiology and Bacterial Genetics at Claude Bernard University Lyon I. Approximately 220 teaching hours per year.

• An average of 211 hours of teaching equivalent TD from 2012 to 2022, including 10% lectures, 10% tutorials, 72% practical work, and 8% HRS. In 2020 and 2021, approximately 100 hours equivalent TD following two consecutive half-time CNRS delegations. Then return to full service in 2022.

• Director of the "Molecular Genetics of Microbes" course unit in the Lyon I Master's in Microbiology since 2016.

• Co-director of the "Microbial Engineering" course unit in the Lyon I Master's in Microbiology since 2016.

• Director of the "Methods and Techniques in Molecular Microbiology" course unit in the Microbiology Bachelor's program since 2022.

• Co-director of the "Molecular Microbiology" course unit in the ENS Lyon Bachelor's program since 2022.

• Director of the E2M2 doctoral school module "Genetic Tools and Mutant Construction in Microorganisms"

• Director or co-director of 3 bacterial genetics practical courses from L3 to M2 level in the Master's in Microbiolo

FUNDING OBTAINED

I have demonstrated a strong ability to secure significant funding for my research. I am currently a partner in the ANR DeCa-P project (2023-2026) with funding of €110k, and I participated in the ANR Tn-Phyto (2020-2024) which brought €205k to my team out of a total of €516k. I also obtained major funding of €150k from the University of Lyon I for a plant imaging system and a CNRS "Chitibact" project of €89k. My collaborations with Inov3PT allowed me to secure €30k for 2022-2023, supplemented by several FR Bienvis research AAP grants. Early in my career, I had already demonstrated this ability by obtaining significant funding of €110k from FRM for my postdoctoral research.

MEDIA APPEARANCES ON SCIENCE AND SOCIETY ISSUES

• Interview with L'Express in 2019 for the article: "Science: The New Obscurantists" (https://www.lexpress.fr/actualite/sciences/sciences-les-nouveaux-obscurantistes_2087668.html)

• Interview with Arrêt sur Images about the peer review process in 2020 (https://www.arretsurimages.net/articles/les-revues-scientifiques-des-medias-comme-les-autres)