Education

College - Neotia Institute Of Technology Management and Science

University - West Bengal University of Technology

Specialization - Biotechnology

Duration - 2009-2013

CGPA - 8.19

Project -

• Title - Computational prediction of upregulation or downregulation of oncogene by microRNA

• Guide - Dr. Jayprakah Chakraborty

• Brief - In this study the involvement of microRNAs in the up-regulation or downregulation of various oncogenes responsible for oral cancer has been studied.

University - SASTRA University

Department - Department of Chemical and Biotechnology

Duration - 2013-2015

DGPA - 8.69

Project:

• Title: Role of Toxin-Antitoxin Systems on bacterial persistence

• Guide: Dr. R. B. Chandra Mohan

• Brief: In this study the effect of deletion of ten type II Toxin-Antitoxin Systems (TAS) on biomass and persistence under various stressed condition is seen. Most of the bacterial pathogens face anaerobic conditions during infections. Hence it is most appropriate to study persistence and biomass under anaerobic conditions. Since TAS are shown to play an active role in persistence, we investigated the role of type II TAS in persistence under antibiotic stress in both aerobic and anaerobic condition. Persister cell formation in various growth phases (mid-log and stationary) and effects of aeration in biomass and persistence is observed and compared. Significant reduction in persister cell formation is observed in TA deleted strain when treated with Ampicillin and Ciprofloxacin. However, in the presence of translational inhibitors, we observed that there is no significant difference in the number of persisters formed between wild type and mutant strains. These observations proved the involvement of type II TAS in bacterial persistence under various growth stages and various stressed conditions.

Institute - Indian Institute of Technology Kharagpur

Department - Department of Biotechnology.

Status - Synopsis given June 2021.

Duration - 2015 - 2021

Doctoral Thesis:

• Title - Elucidating the importance of microRNA-long non-coding RNA-messenger RNA interaction in the regulation of gene expression

• Supervisor - Dr. Ranjit Prasad Bahadur

• Work in Brief: MicroRNAs (miRNAs) are small ncRNAs, which regulate gene expression by inhibiting translation or by degrading messenger RNAs (mRNAs). On the other hand, long non- coding RNAs (lncRNAs) are the transcripts responsible for the up-regulation and down-regulation of transcription. In this study we have computationally predicted miRNAs and lncRNAs from different Viridiplantae species. Targets for these miRNAs have been predicted by psRNATarget Server and the functional annotation of the targets has also been analyzed. To corroborate our computational prediction, we have randomly validated few miRNAs and lncRNAs by qRT-PCR method. We have also experimentally validated few targets of these miRNAs by 5’ RLM-RACE method. Along with that we have done a computational non-coding genome analysis of fifteen viridiplantae families based on their miRNAs and targets. We have also predicted cross-species targets for these miRNAs by psRNATarget Server.