At UC Santa Cruz's Camps lab, I am investigating genetic mutations that confer antibiotic resistance to Fluoroquinolones in Uropathogenic E. coli causing complicated urinary tract infections (UTIs). Utilizing clinical isolates and samples, we employ whole genome sequencing to identify Quinolone Resistance Determining Region (QRDR) mutations. I use Lambda Red mediated recombineering to create isogenic strains, ensuring precise genetic contexts for minimum inhibitory concentration (MIC) assays. Through these assays and statistical analysis, my project aims to elucidate how different QRDR mutations confer resistance and each QRDRs binding affinity to DNA replication enzymes, ultimately informing future novel drug design.