研究業績

2022
The immunoreceptor SLAMF8 promotes the differentiation of follicular dendritic cell-dependent monocytic cells with B cell-activating ability.
Magari M, Nishioka M, Hari T, Ogawa S, Takahashi K, Hatano N, Kanayama N, Futami J, and Tokumitsu H
FEBS Lett. (2022) in press, doi: 10.1002/1873-3468.14468

Substrate recognition by Arg/Pro-rich insert domain in calcium/calmodulin‐dependent protein kinase kinase for target protein kinases.
Kaneshige R, Ohtsuka S, Harada Y, Kawamata I, Magari M, Kanayama N, Hatano N, Sakagami H, and Tokumitsu H
FEBS J. 289, 5971-5984 (2022) doi: 10.1111/febs.16467

Conformation-dependent reversible interaction of Ca²⁺/calmodulin-dependent protein kinase kinase with an inhibitor, TIM-063.
Ohtsuka S, Okumura T, Tabuchi Y, Miyagawa T, Kanayama N, Magari M, Hatano N, Sakagami H, Suizu F, Ishikawa T, and Tokumitsu H
Biochemistry 61, 545-553 (2022) doi: 10.1021/acs.biochem

Oligomerization of Ca²⁺/calmodulin-dependent protein kinase kinase.
Fukumoto Y, Harada Y, Ohtsuka S, Kanayama N, Magari M, Hatano N, Sakagami H, and Tokumitsu H
Biochem. Biophys. Res. Commun. 587, 160-165 (2022)

2021
Identification and Biochemical Characterization of High Mobility Group Protein 20A as a Novel Ca²⁺/S100A6 Target.
Yamamoto M, Kondo R, Hozumi H, Doi S, Denda M, Magari M, Kanayama N, Hatano N, Morishita R, and Tokumitsu H
Biomolecules 11, 510 (2021)

Regulation of the tubulin polymerization-promoting protein by Ca²⁺/S100 proteins.
Doi S, Fujioka N, Ohtsuka S, Kondo R, Yamamoto M, Denda M, Magari M, Kanayama N, Hatano N, Morishita R, Hasegawa T, and Tokumitsu H
Cell Calcium 96, 102404 (2021)

2020
Development and characterization of novel molecular probes for Ca²⁺/calmodulin-dependent protein kinase kinase, derived from STO-609.
Ohtsuka S, Ozeki Y, Fujiwara M, Miyagawa T, Kanayama N, Magari M, Hatano N, Suizu F, Ishikawa T, and Tokumitsu H
Biochemistry 59, 1701-1710 (2020)

Phosphorylation and dephosphorylation of Ca²⁺/calmodulin-dependent protein kinase kinase β at Thr144 in HeLa cells.
Takabatake S, Fukumoto Y, Ohtsuka S, Kanayama N, Magari M, Sakagami H, Hatano N, and Tokumitsu H
Biochem. Biophys. Res. Commun. 525, 251-257 (2020)

SRSF1-3, a splicing and somatic hypermutation regulator, controls transcription of IgV genes via chromatin regulators SATB2, UBN1 and histone variant H3.3.
Singh AK, Tamrakar A, Jaiswal A, Kanayama N, Kodgire P
Mol. Immunol. 119, 69-82 (2020)

2019
Regulation of Ca²⁺/calmodulin-dependent protein kinase kinase β by cAMP signaling.
Takabatake S^*, Ohtsuka S^*, Sugawara T, Hatano N, Kanayama N, Magari M, Sakagami H, and Tokumitsu H
Biochim. Biophys. Acta.- General Subjects 1863, 672-680 (2019)
(equal contribution^*)

Interaction of S100A6 with Target Proteins In Vitro and in Living Cells.
Sakane K, Yamaguchi F, Tsuchiya M, Kondo R, Kanayama N, Magari M, Hatano N, Kobayashi R, and Tokumitsu H
Methods Mol. Biol. 1929, 367-377 (2019)

IL-34 cell surface localization regulated by the 78 kDa glucose-regulated protein facilitates the differentiation of monocytic cells.
Ogawa S, Matsuoka Y, Takada M, Matsui K, Yamane F, Kubota E, Yasuhara S, Hieda K, Kanayama N, Hatano N, Tokumitsu H, and Magari M
J. Biol. Chem. 294, 2386-2396 (2019)

Splicing regulator SRSF1-3 that controls somatic hypermutation of IgV genes interacts with topoisomerase 1 and AID.
Kumar Singh A, Tamrakar A, Jaiswal A, Kanayama N, Agarwal A, Tripathi P, Kodgire P
Mol. Immunol. 116, 63-72 (2019)

2017
AMP-activated protein kinase-mediated feedback phosphorylation controls the Ca²⁺/calmodulin (CaM) dependence of Ca²⁺/CaM-dependent protein kinase kinase β.
Nakanishi A, Hatano N, Fujiwara Y, Sha'ri A, Takabatake S, Akano H, Kanayama N, Magari M, Nozaki N, and Tokumitsu H
J. Biol. Chem. 292, 19804-19813 (2017)

Identification and characterization of a centrosomal protein, FOR20 as a novel S100A6 target.
Sakane K, Nishiguchi M, Denda M, Yamaguchi F, Magari M, Kanayama N, Morishita R, and Tokumitsu H
Biochem. Biophys. Res. Commun. 491, 980-985 (2017)

SRSF1-3 contributes to diversification of the immunoglobulin variable region gene by promoting accumulation of AID in the nucleus.
Kawaguchi Y, Nariki H, Kawamoto N, Kanehiro Y, Miyazaki S, Suzuki M, Magari M, Tokumitsu H and Kanayama N
Biochem. Biophys. Res. Commun. 485, 261-266 (2017)

2016
Differential AMP-activated Protein Kinase (AMPK) Recognition Mechanism of Ca²⁺/Calmodulin-dependent Protein Kinase Kinase Isoforms.
Fujiwara Y, Kawaguchi Y, Fujimoto T, Kanayama N, Magari M, and Tokumitsu H
J. Biol. Chem. 291, 13802-13808 (2016)

Identification of Striated Muscle Activator of Rho Signaling (STARS) as a Novel Calmodulin Target by a Newly Developed Genome-Wide Screen.
Furuya Y, Denda M, Sakane K, Ogusu T, Takahashi S, Magari M, Kanayama N, Morishita R, and
Tokumitsu H
Cell Calcium 60, 32-40 (2016)

2015
Analysis of Distinct Roles of CaMKK Isoforms Using STO-609-Resistant Mutants in Living Cells.
Fujiwara Y, Hiraoka Y, Fujimoto T, Kanayama N, Magari M, and Tokumitsu H
Biochemistry 54, 3969–3977 (2015)

2014
免疫グロブリンの体細胞高頻度突然変異とserine/arginine-rich protein splicing factor (SRSF).
曲正樹, 金山直樹
臨床免疫・アレルギー科 61, 489–495 (2014)

AIDによる免疫グロブリンの体細胞高頻度突然変異誘導と関連分子.
金山直樹, 曲正樹
臨床免疫・アレルギー科 61, 467–471 (2014)

H. CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.
Yamane, F., Nishikawa, Y., Matsui, K., Asakura, M., Iwasaki, E., Watanabe, K., Tanimoto, H., Sano, H., Fujiwara, Y., Stanley, E. R., Kanayama, N., Mabbott, N. A., Magari, M. and Ohmori, H
J Leukoc Biol 95, 19–31 (2014)

2012
抗体遺伝子への突然変異脳を有するニワトリB細胞株の機能拡張によるin vitroモノクローナル抗体作製・改良システム.
金山直樹, 大森 斉
実験医学 31, 1159–1165 (2012)

Activation-induced cytidine deaminase (AID)-dependent somatic hypermutation requires a splice isoform of the serine/arginine-rich (SR) protein SRSF1.
Kanehiro, Y., Todo, K., Negishi, M., Fukuoka, J., Gan, W., Hikasa, T., Kaga, Y., Takemoto, M., Magari, M.,
Li, X., Manley, J. L., Ohmori, H. and Kanayama, N
Proc Natl Acad Sci USA 109, 1216–1221 (2012)

胚中心 B 細胞の分化と濾胞樹状細胞 (特集 B 細胞の機能発現とその制御).
大森齊, 曲正樹, 金山直樹
臨床免疫・アレルギー科 56, 116–122 (2012)

2011
IL-21-dependent B cell death driven by prostaglandin E2, a product secreted from follicular dendritic cells.
Magari, M., Nishikawa, Y., Fujii, Y., Nishio, Y., Watanabe, K., Fujiwara, M., Kanayama, N. and Ohmori H
J Immunol 187, 4210–4218 (2011)

2010
Efficient in vitro antibody generation system using an engineered chicken B cell line, DT40-SW.
Ohmori, H., Magari, M., Kanayama, N., Matsuda, S. and Kanehiro, Y
Journal of Biotechnology 150, 440–441 (2010)

Efficient affinity maturation of antibodies in an engineered chicken B cell line DT40-SW by increasing point mutation.
Kajita, M., Okazawa, T., Ikeda, M., Todo, K., Magari, M., Kanayama, N. and Ohmori, H
J Biosci Bioeng 110, 351–358 (2010)

Enhancement of hypermutation frequency in the chicken B cell line DT40 for efficient diversification of the antibody repertoire.
Magari, M., Kanehiro, Y., Todo, K., Ikeda, M., Kanayama, N. and Ohmori, H
Biochem Biophys Res Commun 396, 353–358 (2010)

Conditional transformation of immunoglobulin mutation pattern from gene conversion into point mutation by controlling XRCC3 expression in the DT40 B cell line.
Kajita, M., Magari, M., Todo, K., Kanayama, N. and Ohmori, H
J Biosci Bioeng 109, 407–410 (2010)

2009
高親和性抗体の産生機構に関する研究とその工学的応用
金山直樹
平成20年度生物工学奨励賞（斎藤賞）. 生物工学会誌 87, 116–122 (2009)

Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression.
Magari, M., Aya, T., Ikeda, M., Todo, K
J Biosci Bioeng 107, 206–209 (2009)

Creation of valuable antibodies by an in vitro antibody generation system using a hypermutating B cell line.
Kanayama, N., Todo, K., Magari, M. and Ohmori, H
YAKUGAKU ZASSHI 129, 11–17 (2009)

2008
Analysis of B cell selection in the germinal center reaction during a T-dependent antibody response at a single cell level.
Okazawa, T., Magari, M., Kimoto, T., Kouyama, E., Ohmori, H. and Kanayama, N
Immunol Lett 117, 96–105 (2008)

2007
Analysis of antigen-stimulated B cell migration into germinal centers during the early stage of a T-dependent immune response.
Kouyama, E., Nishikawa, Y., Okazawa, T., Magari, M., Ohmori, H. and Kanayama, N
Immunol Lett 109, 28–35 (2007)

2006
Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library.
Todo, K., Miyake, K., Magari, M., Kanayama, N. and Ohmori, H
J Biosci Bioeng 102, 478–481 (2006)

Establishment of lymphotoxin beta receptor signaling-dependent cell lines with follicular dendritic cell phenotypes from mouse lymph nodes.
Nishikawa, Y., Hikida, M., Magari, M., Kanayama, N., Mori, M., Kitamura, H., Kurosaki, T. and Ohmori, H
J Immunol 177, 5204–5214 (2006)

変異機能のON/OFF制御可能なB細胞株を用いた抗体および変異タンパク質の作製システム.
大森齊, 藤堂景史, 金山直樹
実験医学 24, 1331–1335 (2006)

Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line.
Magari, M., Ikeda, M., Asakura, M., Kanayama, N., Ogawa, M. and Ohmori H
Immunopharmac immunotoxic 28, 491–500 (2006)

2005
Immunogenicity of an inflammation-associated product, tyrosine nitrated self-proteins.
Ohmori, H. and Kanayama, N
Autoimmunity reviews 4, 224–229 (2005)

Reversible switching of immunoglobulin hypermutation machinery in a chicken B cell line.
Kanayama, N., Todo, K., Reth, M. and Ohmori, H
Biochem Biophys Res Commun 327, 70–75 (2005)

Analysis of marginal zone B cell development in the mouse with limited B cell diversity: role of the antigen receptor signals in the recruitment of B cells to the marginal zone.
Kanayama, N., Cascalho, M. and Ohmori, H
J Immunol 174, 1438–1445 (2005)

Immunogenicity of autologous IgG bearing the inflammation-associated marker 3-nitrotyrosine.
Ohmori, H., Oka, M., Nishikawa, Y., Shigemitsu, H., Takeuchi, M., Magari, M. and Kanayama, N
Immunol Lett 96, 47–54(2005)

2003
Mechanisms leading to autoantibody production: link between inflammation and autoimmunity.
Ohmori, H. and Kanayama, N
Current drug targets Inflammation and allergy 2, 232–241 (2003)

2002
B cell selection and affinity maturation during an antibody response in the mouse with limited B cell diversity.
Kanayama, N., Kimoto, T., Todo, K., Nishikawa, Y., Hikida, M., Magari, M., Cascalho, M. and Ohmori, H
J Immunol 169, 6865–6874 (2002)

Role for complement receptors (CD21/CD35) in the regulation of recombination activating gene expression in murine peripheral B cells.
Ohmori, H., Magari, M., Nakayama, Y., Kanayama, N. and Hikida, M
Immunol Lett 83, 95–99 (2002)

Contribution of light chain rearrangement in peripheral B cells to the generation of high-affinity antibodies.
Magari, M., Sawatari, T., Kawano, Y., Cascalho, M., Wabl, M., Kanayama, N., Hikida, M. and Ohmori, H
Eur J Immunol 32, 957–966 (2002)

2001
Use of secondarily revised VH genes in IgE antibodies produced in mice infected with the nematode Nippostrongylus brasiliensis.
Kanayama, N., Hukue, C., Magari, M., Ohtani, K., Hikida, M., Yamada, M., Matsuda, S. and Ohmori, H
Immunol Lett 77, 181–186 (2001)

RESTORATION OF IMMUNOCYTE FUNCTIONS BY THYMOSIN α1 IN CYCLOPHOSPHAMIDE-INDUCED IMMUNODEFICIENT MICE.
Ohmori, H., Kamo, M., Yamakoshi, K., Nitta, M. H. M., Hikida, M. and Kanayama, N
Immunopharmac immunotoxic 23, 75–82 (2001)

2000
Prevention of collagen-induced arthritis in DBA/1 mice by oral administration of AZ-9, a bacterial polysaccharide from Klebsiella oxytoca.
Sugihara, R., Yoshimura, M., Mori, M., Kanayama, N., Hikida, M. and Ohmori, H
Immunopharmacology 49, 325–333 (2000)

Selective augmenting effects of nitric oxide on antigen-specific IgE response in mice.
Ohmori, H., Egusa, H., Ueura, N., Matsumoto, Y., Kanayama, N. and Hikida, M
Immunopharmacology 46, 55–63 (2000)

An n-alkane-responsive promoter element found in the gene encoding the peroxisomal protein of Candida tropicalis does not contain a C(6) zinc cluster DNA-binding motif.

Kanai, T., Hara, A., Kanayama, N., Ueda, M. and Tanaka, A.

J Bacteriol 182, 2492–2497 (2000).

Genetic evaluation of peroxisomal and cytosolic acetoacetyl-CoA thiolase isozymes in n-alkane-assimilating diploid yeast, Candida tropicalis.

Ueda, M., Kanayama, N. and Tanaka, A.

Cell Biochem Biophys 32 Spring, 285–290 (2000).

1999

Construction of an autonomously replicating plasmid in n-alkane-assimilating yeast, Candida tropicalis.

Hara, A., Ueda, M., Matsui, T., Furuhashi, K., Kanayama, N. and Tanaka, A.

J Biosci Bioeng 87, 717–720 (1999).

1998

Genetic evaluation of physiological functions of thiolase isoenzymes in the n-alkalane-assimilating yeast Candida tropicalis.

Kanayama, N., Ueda, M., Atomi, H. and Tanaka, A.

J Bacteriol 180, 690–698 (1998).

1997

Expression of acetoacetyl-CoA thiolase isozyme genes of n-alkane-assimilating yeast, Candida tropicalis: isozymes in two intracellular compartments are derived from the same genes.

Kanayama, N., Himeda, Y., Atomi, H., Ueda, M. and Tanaka, A.

J. Biochem 122, 616–621 (1997).

Derepression of gene expression mediated by the 5' upstream region of the isocitrate lyase gene of Candida tropicalis is controlled by two distinct regulatory pathways in Saccharomyces cerevisiae.

Umemura, K., Atomi, H., Kanai, T., Takeshita, S., Kanayama, N., Ueda, M. and Tanaka, A.

Eur J Biochem 243, 748–752 (1997).

1995

3-Ketoacyl CoA thiolases of a yeast, Candida tropicalis. Properties and functions.

Tanaka, A., Kurihara, T., Kanayama, N., Atomi, H. and Ueda, M.

Ann N Y Acad Sci 750, 39–43 (1995).

1994

Comparison of molecular structures and regulation of biosynthesis of unique thiolase isozymes localized only in peroxisomes of n-alkane-utilizable yeast, Candida tropicalis.

Kanayama, N., Ueda, M., Atomi, H., Kurihara, T., Kondo, J., Teranishi, Y., and Tanaka, T.

J Ferment Bioeng 78, 273–278 (1994).

Molecular evolution of yeast thiolase isozymesT.

Kanayama, N., Ueda, M., Atomi, H., Kurihara, T. and Tanaka, A.

J Ferment Bioeng 78, 279–282 (1994).

1992

Peroxisomal acetoacetyl-CoA thiolase of an n-alkane-utilizing yeast, Candida tropicalis.

Kurihara, T., Ueda, M., Kanayama, N., Kondo, J., Teranishi, Y. and Tanaka, A.

Eur J Biochem 210, 999–1005 (1992).