Manuela Zoonens

Research articles:

35. Influence of Hydrophobic Groups Attached to Amphipathic Polymers on the Solubilization of Membrane Proteins along with Their Lipids. Marconnet A, Michon B, Prost B, Solgadi A, Le Bon C, Giusti F, Tribet C, Zoonens M. Anal Chem. 2022 Oct 18;94(41):14151-14158. doi: 10.1021/acs.analchem.2c01746. Epub 2022 Oct 6. PMID: 36200347

34. Author Correction: Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis. Higgins AJ, Flynn AJ, Marconnet A, Musgrove LJ, Postis VLG, Lippiat JD, Chung CW, Ceska T, Zoonens M, Sobott F, Muench SP. Commun Biol. 2022 Jan 5;5(1):1. doi: 10.1038/s42003-021-02997-z. PMID: 34987157 Free PMC article. No abstract available.

33. Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis.Higgins AJ, Flynn AJ, Marconnet A, Musgrove LJ, Postis VLG, Lippiat JD, Chung CW, Ceska T, Zoonens M, Sobott F, Muench SP. Commun Biol. 2021 Nov 25;4(1):1337. doi: 10.1038/s42003-021-02834-3. PMID: 34824357 Free PMC article.

32. Full-length G glycoprotein directly extracted from rabies virus with detergent and then stabilized by amphipols in liquid and freeze-dried forms.Clénet D, Clavier L, Strobbe B, Le Bon C, Zoonens M, Saulnier A. Biotechnol Bioeng. 2021 Nov;118(11):4317-4330. doi: 10.1002/bit.27900. Epub 2021 Aug 5. PMID: 34297405 Free PMC article.

31. Amphipathic environments for determining the structure of membrane proteins by single-particle electron cryo-microscopy. Le Bon C, Michon B, Popot JL, Zoonens M. Q Rev Biophys. 2021 Mar 31;54:e6. doi: 10.1017/S0033583521000044. PMID: 33785082 Free article.

30. Solubilization and stabilization of membrane proteins by cycloalkane-modified amphiphilic polymers. Marconnet A, Michon B, Le Bon C, Giusti F, Tribet C, Zoonens M. Biomacromolecules. 2020 Jun 30. doi: 10.1021/acs.biomac.0c00929. Online ahead of print. PMID: 32602705 

29. Improved protection against Chlamydia muridarum using the native major outer membrane protein trapped in Resiquimod-carrying amphipols and effects in protection with addition of a Th1 (CpG-1826) and a Th2 (Montanide ISA 720) adjuvant. Tifrea DF, Pal S, le Bon C, Cocco MJ, Zoonens M, de la Maza LM. Vaccine. 2020 Jun 9;38(28):4412-4422. doi: 10.1016/j.vaccine.2020.04.065. Epub 2020 Apr 30. PMID: 32386746 

28. BAmSA: Visualising transmembrane regions in protein complexes using biotinylated amphipols and electron microscopy. Perry TN, Souabni H, Rapisarda C, Fronzes R, Giusti F, Popot JL, Zoonens M, Gubellini F. Biochim Biophys Acta Biomembr. 2019 Feb 1;1861(2):466-477. doi: 10.1016/j.bbamem.2018.11.004. Epub 2018 Nov 13. PMID: 30444973 Free article. 

27. Co-delivery of amphipol-conjugated adjuvant with antigen, and adjuvant combinations, enhance immune protection elicited by a membrane protein-based vaccine against a mucosal challenge with Chlamydia. Tifrea DF, Pal S, Le Bon C, Giusti F, Popot JL, Cocco MJ, Zoonens M, de la Maza LM. Vaccine. 2018 Oct 29;36(45):6640-6649. doi: 10.1016/j.vaccine.2018.09.055. Epub 2018 Oct 4. PMID: 30293763 

26. Stabilization of a Membrane-Associated Amyloid-β Oligomer for Its Validation in Alzheimer's Disease. Serra-Batiste M, Tolchard J, Giusti F, Zoonens M, Carulla N. Front Mol Biosci. 2018 Apr 19;5:38. doi: 10.3389/fmolb.2018.00038. eCollection 2018. PMID: 29725595 Free PMC article. 

25. Folding and stabilizing membrane proteins in amphipol A8-35. Le Bon C, Marconnet A, Masscheleyn S, Popot JL, Zoonens M. Methods. 2018 Sep 1;147:95-105. doi: 10.1016/j.ymeth.2018.04.012. Epub 2018 Apr 18. PMID: 29678587 Review. 

24. Systematic analysis of the use of amphipathic polymers for studies of outer membrane proteins using mass spectrometry. Watkinson TG, Calabrese AN, Giusti F, Zoonens M, Radford SE, Ashcroft AE. Int J Mass Spectrom. 2015 Nov 30;391:54-61. doi: 10.1016/j.ijms.2015.06.017. PMID: 26869850 Free PMC article. 

23. Synthesis of a Polyhistidine-bearing Amphipol and its Use for Immobilizing Membrane Proteins. Giusti F, Kessler P, Hansen RW, Della Pia EA, Le Bon C, Mourier G, Popot JL, Martinez KL, Zoonens M. Biomacromolecules. 2015 Dec 14;16(12):3751-61. doi: 10.1021/acs.biomac.5b01010. Epub 2015 Nov 5. PMID: 26492302 

22. Amphipols for each season. Zoonens M, Popot JL. J Membr Biol. 2014 Oct;247(9-10):759-96. doi: 10.1007/s00232-014-9666-8. Epub 2014 Jun 27. PMID: 24969706 Free PMC article. Review. 

21. Amphipol-trapped ExbB-ExbD membrane protein complex from Escherichia coli: a biochemical and structural case study. Sverzhinsky A, Qian S, Yang L, Allaire M, Moraes I, Ma D, Chung JW, Zoonens M, Popot JL, Coulton JW. J Membr Biol. 2014 Oct;247(9-10):1005-18. doi: 10.1007/s00232-014-9678-4. Epub 2014 May 27. PMID: 24862870 

20. Synthesis of an oligonucleotide-derivatized amphipol and its use to trap and immobilize membrane proteins. Le Bon C, Della Pia EA, Giusti F, Lloret N, Zoonens M, Martinez KL, Popot JL. Nucleic Acids Res. 2014 Jun;42(10):e83. doi: 10.1093/nar/gku250. Epub 2014 Apr 17. PMID: 24744236 Free PMC article. 

19. Functionalized amphipols: a versatile toolbox suitable for applications of membrane proteins in synthetic biology. Della Pia EA, Hansen RW, Zoonens M, Martinez KL. J Membr Biol. 2014 Oct;247(9-10):815-26. doi: 10.1007/s00232-014-9663-y. Epub 2014 Apr 13. PMID: 24728227 Review. 

18. The use of amphipols for solution NMR studies of membrane proteins: advantages and constraints as compared to other solubilizing media. Planchard N, Point É, Dahmane T, Giusti F, Renault M, Le Bon C, Durand G, Milon A, Guittet É, Zoonens M, Popot JL, Catoire LJ. J Membr Biol. 2014 Oct;247(9-10):827-42. doi: 10.1007/s00232-014-9654-z. Epub 2014 Mar 28. PMID: 24676477 

17. How amphipols embed membrane proteins: global solvent accessibility and interaction with a flexible protein terminus. Etzkorn M, Zoonens M, Catoire LJ, Popot JL, Hiller S. J Membr Biol. 2014 Oct;247(9-10):965-70. doi: 10.1007/s00232-014-9657-9. Epub 2014 Mar 26. PMID: 24668145 

16. A step closer to membrane protein multiplexed nanoarrays using biotin-doped polypyrrole. Della Pia EA, Holm JV, Lloret N, Le Bon C, Popot JL, Zoonens M, Nygård J, Martinez KL. ACS Nano. 2014 Feb 25;8(2):1844-53. doi: 10.1021/nn406252h. Epub 2014 Feb 4. PMID: 24476392 Free PMC article. 

15. Dangerous liaisons between detergents and membrane proteins. The case of mitochondrial uncoupling protein 2. Zoonens M, Comer J, Masscheleyn S, Pebay-Peyroula E, Chipot C, Miroux B, Dehez F. J Am Chem Soc. 2013 Oct 9;135(40):15174-82. doi: 10.1021/ja407424v. Epub 2013 Sep 26. PMID: 24021091 

14. Assaying the proton transport and regulation of UCP1 using solid supported membranes. Blesneac I, Ravaud S, Machillot P, Zoonens M, Masscheylen S, Miroux B, Vivaudou M, Pebay-Peyroula E. Eur Biophys J. 2012 Aug;41(8):675-9. doi: 10.1007/s00249-012-0844-2. Epub 2012 Jul 31. PMID: 22847775 

13. Production of UCP1 a membrane protein from the inner mitochondrial membrane using the cell free expression system in the presence of a fluorinated surfactant. Blesneac I, Ravaud S, Juillan-Binard C, Barret LA, Zoonens M, Polidori A, Miroux B, Pucci B, Pebay-Peyroula E. Biochim Biophys Acta. 2012 Mar;1818(3):798-805. doi: 10.1016/j.bbamem.2011.12.016. Epub 2011 Dec 27. PMID: 22226924 

12. Amphipols from A to Z. Popot J-L, Althoff T, Bagnard D, Banères JL, Bazzacco P, Billon-Denis E, Catoire LJ, Champeil P, Charvolin D, Cocco MJ, Crémel G, Dahmane T, de la Maza LM, Ebel C, Gabel F, Giusti F, Gohon Y, Goormaghtigh E, Guittet E, Kleinschmidt JH, Kühlbrandt W, Le Bon C, Martinez KL, Picard M, Pucci B, Sachs JN, Tribet C, van Heijenoort C, Wien F, Zito F, Zoonens M. Annu Rev Biophys. 2011, 40:379-408.

11. Expression of membrane proteins at the Escherichia coli membrane for structural studies. Zoonens M, Miroux B. Methods Mol Biol. 2010;601:49-66. doi: 10.1007/978-1-60761-344-2_4. PMID: 20099139 

10. Solution NMR mapping of water-accessible residues in the transmembrane beta-barrel of OmpX. Catoire LJ, Zoonens M, van Heijenoort C, Giusti F, Guittet E, Popot JL. Eur Biophys J. 2010 Mar;39(4):623-30. doi: 10.1007/s00249-009-0513-2. Epub 2009 Jul 29. PMID: 19639312 

9. Inter- and intramolecular contacts in a membrane protein/surfactant complex observed by heteronuclear dipole-to-dipole cross-relaxation. Catoire LJ, Zoonens M, van Heijenoort C, Giusti F, Popot JL, Guittet E. J Magn Reson. 2009 Mar;197(1):91-5. doi: 10.1016/j.jmr.2008.11.017. Epub 2008 Dec 6. PMID: 19101186 

8. Bilayer interactions of pHLIP, a peptide that can deliver drugs and target tumors. Zoonens M, Reshetnyak YK, Engelman DM. Biophys J. 2008 Jul;95(1):225-35. doi: 10.1529/biophysj.107.124156. Epub 2008 Mar 21. PMID: 18359793 Free PMC article. 

7. Dynamics of membrane protein/amphipol association studied by Förster resonance energy transfer: implications for in vitro studies of amphipol-stabilized membrane proteins. Zoonens M, Giusti F, Zito F, Popot JL. Biochemistry. 2007 Sep 11;46(36):10392-404. doi: 10.1021/bi7007596. Epub 2007 Aug 18. PMID: 17705558 

6. Free-standing films of fluorinated surfactants as 2D matrices for organizing detergent-solubilized membrane proteins. Petkova V, Benattar JJ, Zoonens M, Zito F, Popot JL, Polidori A, Jasseron S, Pucci B. Langmuir. 2007 Apr 10;23(8):4303-9. doi: 10.1021/la063249o. Epub 2007 Mar 17. PMID: 17367172 

5. Dynamic helix interactions in transmembrane signaling. Matthews EE, Zoonens M, Engelman DM. Cell. 2006 Nov 3;127(3):447-50. doi: 10.1016/j.cell.2006.10.016. PMID: 17081964 Free article. 

4. Reconstitution and alignment by a magnetic field of a beta-barrel membrane protein in bicelles. Triba MN, Zoonens M, Popot JL, Devaux PF, Warschawski DE. Eur Biophys J. 2006 Feb;35(3):268-75. doi: 10.1007/s00249-005-0014-x. Epub 2005 Sep 27. PMID: 16187128 

3. NMR study of a membrane protein in detergent-free aqueous solution. Zoonens M, Catoire LJ, Giusti F, Popot JL. Proc Natl Acad Sci U S A. 2005 Jun 21;102(25):8893-8. doi: 10.1073/pnas.0503750102. Epub 2005 Jun 14. PMID: 15956183 Free PMC article. 

2. Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method. Ferrage F, Zoonens M, Warschawski DE, Popot JL, Bodenhausen G. J Am Chem Soc. 2003 Mar 5;125(9):2541-5. doi: 10.1021/ja0211407. PMID: 12603142 

1. Amphipols : polymeric surfactants for membrane biology research. Popot J-L, Berry EA, Charvolin D, Creuzenet C, Ebel C, Engelman DM, Flötenmeyer M, Giusti F, Gohon Y, Hong Q, Lakey JH, Leonard K, Shuman HA, Timmins P, Warschawski DE, Zito F, Zoonens M, Pucci B, Tribet C. Cell Mol Life Sci. 2003, 60(8):1559-74

Book chapters:

3. Amphipols: a general introduction and some protocols. Zoonens M, Zito F, Martinez KL, Popot J-L. MEMBRANE PROTEINS PRODUCTION FOR STRUCTURAL ANALYSIS. 2014, Springer, New-York, 173-203.

2. Membrane protein production in Escherichia coli: overview and protocols. Hattab G, Suisse A, Ilioaia O, Casiraghi M, Dezi M, Warnet X, Warschawski D, Moncoq K, Zoonens M, Miroux B. MEMBRANE PROTEINS PRODUCTION FOR STRUCTURAL ANALYSIS. 2014,Springer, New-York, 87-106.

1. Amphipols: Strategies for an improved PS2 environment in detergent-free aqueous solution. Nowaczyk M, Oworah-Nkruma R, Zoonens M, Rogner M, Popot J-L. Biohydrogen III : Renewable energy system by biological solar energy conversion. 2004, 151-159.