Publications

2024

Svetlov, M.S., Dunand, C.F., Nakamoto, J.A., Atkinson, G.C., Safdari, H.A., Wilson, D.N., Vázquez-Laslop, N, Mankin, A.S., (2024) Peptidyl-tRNA hydrolase is the nascent chain release factor in bacterial ribosome-associated quality control. Mol Cell. 84, 715-726. 

Judd, H.N.G., Martínez, A.K., Klepacki, D., Vázquez-Laslop, N., Sachs, M.S., Cruz-Vera, L.R. (2024) Functional domains of a ribosome arresting peptide are affected by surrounding nonconserved residues. J. Biol. Chem. 300, 105780

2023

Skowron, K. J., Baliga, C., Johnson, T., Kremiller, K. M., Castroverde, A., Dean, T. T., Allen, A. C., Lopez-Hernandez, A. M., Aleksandrova, E. V., Klepacki, D., Mankin, A. S., Polikanov, Y. S., Moore, T. W. (2023). Structure-Activity Relationships of the Antimicrobial Peptide Natural Product Apidaecin. J. Med, Chem., 66, 11831–11842. 

Vàzquez-Laslop, N. & Polikanov, Y.S. (2023) Motif-ation matters. Nat. Chem. Biol. 19, 1044-1045

Wu, K. J. Y., Klepacki, D., Mankin, A. S., & Myers, A. G. (2023). A method for tritiation of iboxamycin permits measurement of its ribosomal binding. Bioorg. & Med. Chem. Lett., 91, 129364.

Mangano, K., Klepacki, D., Ohanmu, I., Baliga, C., Huang, W., Brakel, A., Krizsan, A., Polikanov, Y. S., Hoffmann, R., Vázquez-Laslop, N., & Mankin, A. S. (2023). Inhibition of translation termination by the antimicrobial peptide Drosocin. Nat. Chem. Biol., 19, 1082–1090. 

2022

Mangano, K., Marks, J., Klepacki, D., Saha, C.K., Atkinson, G.C., Vázquez-Laslop, N., Mankin, A.S. (2022) Context-based sensing of orthosomycin antibiotics by the translating ribosome Nat. Chem. Biol. 18, 1277-1286.

Zhang, Y., Aleksashin, N. A., Klepacki, D., Anderson, C., Vázquez-Laslop, N., Gross, C. A., Mankin, A.S. (2022) The context of the ribosome binding site in mRNAs defines specificity of action of kasugamycin, an inhibitor of translation initiation Proc. Natl. Acad. Sci. USA 119, e2118553119.

Tsai, K., Stojkovic, V., Lee, D.J., Young, I.D., Szal, T., Klepacki, D., Vázquez-Laslop, N., Mankin, A.S., Fraser, J.S., Fujimori, D.G. (2022) Structural basis for context-specific inhibition of translation by oxazolidinone antibiotics. Nat. Struct. Molec. Biol. 29, 162-171.

Mitcheltree, M.J., Pisipati, A., Syroegin, E.A., Silvestre, K.J., Klepacki, D., Mason, J.D., Terwilliger, D.W., Testolin, G., Pote, A.R., Wu, K.J.Y., Ladley, R.P., Chatman, K., Mankin, A.S., Polikanov, Y.S., Myers, A.G. (2022) A synthetic antibiotic class overcoming bacterial multidrug resistance. Nature 599, 507-512.

Vázquez-Laslop, N., Sharma, C.M., Mankin, A.S., Buskirk, A.R. (2022) Identifying small open reading frames in prokaryotes with ribosome profiling. J. Bacteriol. 204, e00294-21.

Laczkovich, I., Mangano, K., Shao, X., Hockenberry, A.J., Gao, Y., Mankin, A., Vàzquez-Laslop, N., and Federle, M.J. (2022) Discovery of unannotated small open reading frames in Streptococcus pneumoniae D39 involved in quorum sensing and virulence using ribosome profiling. mBio 13, e0124722.

2021

Svetlov, M. S., Syroegin, E. A., Aleksandrova, E. V., Atkinson, G. C., Gregory, S. T., Mankin, A. S., Polikanov, Y. S. (2021) Structure of Erm-modified 70S ribosome reveals the mechanism of macrolide resistance. Nat. Chem. Biol. 17, 412-420.

Svetlov, M.S., Koller, T.O., Meydan, S., Shankar, V., Klepacki, D., Polacek, N., Guydosh, N.R., Vázquez-Laslop, N., Wilson, D.N., Mankin, A.S. (2021) Context-specific action of macrolide antibiotics on the eukaryotic ribosome Nat. Commun. 12, 2803.

Meydan, S., Klepacki, D., Mankin, A.S., Vázquez-Laslop, N. (2021) Identification of translation start sites in bacterial genomes. Methods Mol. Biol. 2252, 27-55.

Beckert, B., Leroy, E.C., Sothiselvam, S., Bock, L.V., Svetlov. M.S., Graf, M., Arenz, S., Abdelshahid, M., Seip, B., Grubmüller, H., Mankin, A.S., Innis, C.A., Vázquez-Laslop, N., Wilson, D.N. (2021) Structural and mechanistic basis for translation inhibition by macrolide and ketolide antibiotics Nat. Commun. 12, 4466.

Baliga, C., Brown, TY, Florin, T., Colon, S., Shah, V., Skowron, K.J., Kefi, A., Szal, T., Klepacki, D., Moore, T.W., Vázquez-Laslop, N., Mankin, A.S. (2021) Structure of Erm-modified 70S ribosome reveals the mechanism of macrolide resistance. Proc. Natl. Acad. Sci. USA, 118, e2026465118.

Svetlov, M.S, Syroegin, E.A., Aleksandrova, E.V., Atkinson, G.C., Gregory, S.T., Mankin, A.S. Polikanov, Y.S. (2021) Structure of Erm-modified 70S ribosome reveals the mechanism of macrolide resistance. Nature Chem. Biol. 17, 412-420.

2020

Mangano, K., Florin, T., Shao, X., Klepacki, D., Chelysheva, I., Ignatova, Z., Gao, Y., Mankin, A.S., Vázquez-Laslop, N. (2020) Genome-wide effects of the antimicrobial peptide apidaecin on translation termination in bacteria eLife, 9:e62655

Mankin, A.S. Frameshifted proteins of a given gene are unlikely to retain the same function. (2020) RNA, 26, 1301-1302.

Huang, S., Aleksashin, N.A., Loveland, A.B., Klepacki, D., Reier, K., Kefi, A., Szal, T., Remme, J., Jaeger, L., Vázquez-Laslop, N., Korostelev, A.A., Mankin, A.S (2020) Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly. Nat Commun, 10, 1038.

Aleksashin, N.A., Szal, T., d’Aquino, A.E., Jewett, M.C., Vázquez-Laslop, N., Mankin, A.S (2020) A fully orthogonal system for protein synthesis in bacterial cells. Nat Commun 11, 1858.

Svetlov, M.S., Cohen, S., Alsuhebany, N., Vázquez-Laslop, N., Mankin, A.S. (2020) A long-distance rRNA base-pair impacts the ability of macrolide antibiotics to kill bacteria. Proc. Natl. Acad. Sci. USA, 117, 1971-1975.

2019

Choi, J., Marks, J., Zhang, J., Chen, D.-H., Wang, J., Vázquez-Laslop, N., Mankin, A.S., Puglisi, J.D. (2019) Dynamics of the context-specific translation arrest by chloramphenicol and linezolid. Nature Chem. Biol. 16, 310-317.

Carlson, E.D., d'Aquino, A.E., Kim, D.S., Fulk, E.M., Hoang, K., Szal, T., Mankin, A.S., Jewett, M.C. (2019) Engineered ribosomes with tethered subunits for expanding biological function. Nat. Commun., 10, 3920.

Meydan, S., Marks, J., Klepacki, D., Shrama, V., Baranov, V.P., Firth, A.E., Margus, T., Kefi, A., Vázquez-Laslop, N., Mankin, A.S. (2019) Retapamulin-assisted ribosome profiling reveals the alternative bacterial proteome. Mol Cell, 74, 1-13. 

Aleksashin, N.A., Leppik, M., HockenberryA.J., Klepacki, D., Vázquez-Laslop, N., Jewett, M.C., Remme, J., Mankin, A.S. (2019) Assembly and functionality of the ribosome with tethered subunits. Nat. Commun., 10(1):930. 

2018

Vázquez-Laslop, N., Mankin, A.S. (2018) How macrolide antibiotics work. Trends Biochem. Sci. 43, 668-684.

Vázquez-Laslop, N., Mankin, A.S. (2018) Context-specific action of ribosomal antibiotics. Ann. Rev. Microbiol. 72, 185-207.

Meydan, S., Vázquez-Laslop, N., Mankin, A.S. (2018) Genes within genes in bacterial genomes. In Regulating with RNA in Bacteria and Archaea (Storz, G. and Papenfort, K., eds) ASM Press, Washington DC, pp. 133-154.

Pantel, L., Florin, T., Dobosz-Bartoszek, M., Racine E., Sarciaux, M., Serri, M., Houard, J., Campagne, J.M., de Figueiredo, R.M., Midrier, C., Gaudriault, S., Givaudan, A., Lanois, A., Forst, S., Aumelas, A., Cotteaux-Lautard, C., Bolla, J.M., Vingsbo Lundberg, C., Huseby, D.L., Hughes, D., Villain-Guillot, P., Mankin, A.S., Polikanov, Y.S., Gualtieri, M. (2018) Odilorhabdins, antibacterial agents that cause miscoding by binding at a new ribosomal site. Mol. Cell 70, 83-94

Tereshchenkov, A.G., Dobosz-Bartoszek, M., Osterman, I.A., Marks, J., Sergeeva, V.A., Kasatsky, P., Komarova, E.S., Stavrianidi, A.N., Rodin, I.A., Konevega, A.L., Sergiev, P.V., Sumbatyan, N.V., Mankin, A.S., Bogdanov, A.A., Polikanov, Y.S. (2018) Binding and action of amino acid analogs of chloramphenicol upon the bacterial ribosome. J. Mol. Biol. 430, 842-852.

2017

Svetlov, M.S., Vázquez-Laslop, N., Mankin, A.S. (2017) Kinetics of drug–ribosome interactions defines the cidality of macrolide antibiotics Proc. Natl. Acad. Sci. USA 114, 13673-13678 

Florin, T., Maracci, C., Graf, M., Karki, P., Klepacki, D., Berninghausen, O., Beckmann, R., Vázquez-Laslop, N., Wilson, D.N., Rodnina, M.V., Mankin, A.S. (2017) An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome. Nat. Struct. Molec. Biol., 24, 752-757 (Highlighted on the cover, News release, UIC Press).

Almutairi, M., Svetlov, M.S., Hansen, D.A., Khabibullina, N.F., Klepacki, D., Kang, H-Y., Sherman, D.H., Vázquez-Laslop, N., Polikanov, Y.S., Mankin, A.S. (2017) Co-produced natural ketolides methymycin and pikromycin inhibit bacterial growth by preventing synthesis of a limited number of proteins. Nucl. Acids Res., 45, 9573-9582.

Meydan, S., Klepacki, D., Karthikeyan, S., Thomas, P., Jones, J.E., Khan, Y., Briggs, J., Dinman, J.D., Vázquez-Laslop, N., Mankin, A.S. (2017) Programmed ribosomal frameshifting generates a copper transporter and a copper chaperone from the same gene. Mol. Cell, 65, 207-219 

2016

Marks, J., Kannan, K., Roncase, E. J., Klepacki, D., Kefi, A., Orelle, C., Vázquez-Laslop, N., Mankin, A.S. (2016) Context-specific inhibition of translation by ribosomal antibiotics targeting the peptidyl transferase center. Proc. Natl. Acad. Sci. USA, 113, 12150-12155 

Sothiselvam, S., Neuner, S., Rigger, L., Klepacki, D., Micura, R., Vazquez-Laslop, N., Mankin, A.S. (2016) Binding of macrolide antibiotics compels the ribosome to discriminate against specific substrates based on their charge and size. Cell Reports, 16, 1789-1799.

Gupta, P., Liu, B., Klepacki, D., Gupta, V., Schulten K., Mankin, A.S., Vazquez-Laslop, N. (2016) The nascent polypeptide assists the ribosome in discriminating between chemically distinct molecules. Nature Chem. Biol.12, 153-158. 

Gagnon, M.G., Roy, R.N., Lomakin, I.B., Florin, T., Mankin, A.S., Steitz, T.A. (2016) Structures of proline-rich peptides bound to the ribosome reveal a common mechanism of protein synthesis inhibition. Nucl. Acids Res. 44, 2439-2450.

2015

Almutairi, M.M., Park, S.R., Rose, S., Hansen, D.A., Vázquez-Laslop, N., Douthwaite, S., Sherman, D.H., Mankin, A.S. (2015) Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides. Proc. Natl. Acad. Sci. USA 112, 12956-12961.

Orelle, C., Carlson, E.D., Szal, T., Florin, T., Jewett, M.C., Mankin, A.S. (2015) Protein synthesis by ribosomes with tethered subunits. Nature 524, 119-124. 

2014

Kannan, K., Kanabar, P., Schryer, D., Florin, T., Oh, E., Bahroos, N., Tenson, T., Weissman, J.S., Mankin, A.S. (2014) The general mode of translation inhibition by macrolide antibiotics. Proc. Natl. Acad. Sci. USA 111, 15958-15963.

Polikanov, Y.S., Osterman, I.A., Szal, T., Tashlitsky, V.N., Serebryakova, M.V., Kusochek, P., Bulkley, D., Malanicheva, I.A., Efimenko, T.A., Efremenkova, O.V., Konevega, A.L., Shaw, K.J., Bogdanov, A.A., Rodnina, M.V., Dontsova, O.A., Mankin, A.S., Steitz, T.A., Sergiev. P.V. (2014) Amicoumacin A inhibits translation by stabilizing mRNA interaction with the ribosome. Mol. Cell 56, 531-540.

Polikanov, Y.S., Szal, T., Jiang, F., Gupta, P., Matsuda, R., Shiozuka, M., Steitz, T.A., Vázquez-Laslop N., Mankin, A.S. (2014) Negamycin interferes with decoding and translocation by simultaneous interaction with rRNA and tRNA. Mol. Cell 56, 541-550.

Sothiselvam, S., Liu, B., Han, W., Ramu, H., Klepacki, D., Atkinson, G.C., Brauer, A., Remm, M., Tenson, T., Schulten, K., Vázquez-Laslop, N., Mankin, A.S. (2014) Macrolide antibiotics allosterically predispose the ribosome for translation arrest. Proc. Natl. Acad. Sci. USA 111, 9804-9809.

Vázquez-Laslop, N., Mankin, A.S. (2014) Protein accounting in the cellular economy. Cell 157, 529-531.

Vázquez-Laslop, N., Mankin, A.S. (2014) Triggering peptide-dependent translation arrest by small molecules: ribosome stalling modulated by antibiotics. In: Regulatory Nascent Polypeptides (Ito, K., ed). Springer (Tokyo), pp. 165-186.

Arenz, S., Ramu, H., Gupta, P., Berninghausen, O., Beckmann, R., Vázquez-Laslop, N., Mankin, A.S., Wilson, D.N. (2014) Molecular basis for erythromycin-dependent ribosome stalling during translation of the ErmBL leader peptide. Nat. Commun. 5, 3501.

Martínez, A.K., Gordon, E., Sengupta, A., Shirole, N., Klepacki, D., Martinez-Garriga, B., Brown, L.M., Benedik, M.J., Yanofsky, C., Mankin, A.S., Vazquez-Laslop, N., Sachs, M.S., Cruz-Vera, L.R. (2014) Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan. Nucl. Acids Res., 42, 1245-1256.

2013

Gupta, P., Kannan, K., Mankin, A.S., Vázquez-Laslop, N. (2013) Regulation of gene expression by macrolide-induced ribosomal frameshifting. Mol. Cell 52, 629-642. 

Orelle, C., Carlson, S., Kaushal, B., Almutairi, M.M., Liu, H., Ochabowicz, A., Quan, S., Pham, V.C., Squires, C.L., Murphy, B.T., Mankin, A.S. (2013) Tools for characterizing bacterial protein synthesis inhibitors. Antimicrob. Agents Chemother. 57, 5994-6004.

Gupta, P., Sothiselvam, S., Vázquez-Laslop, N., Mankin, A. S. (2013) Deregulation of translation due to posttranscriptional modification of rRNA explains why erm genes are inducible. Nat. Commun. 4, 1984.

Orelle, C., Szal, T., Klepacki, D., Shaw, K.J., Vázquez-Laslop, N., Mankin, A.S. (2013) Identifying the targets of aminoacyl-tRNA synthetase inhibitors by primer extension inhibition. Nucl. Acids Res. 10, 526.

LaMarre, J., Mendes, R.E., Szal, T., Schwarz, S., Jones, R.N., Mankin, A.S. (2013) The genetic environment of the cfr gene and the presence of other mechanisms account for the very high linezolid resistance of the Staphylococcus epidermidis isolate 426-3147L. Antimicrob. Agents Chemother 57, 1173-1179.

2012

Kannan, K., Vázquez-Laslop, N., Mankin, A. S. (2012) Selective proteins synthesis by ribosomes with a drug-obstructed exit tunnel. Cell, 151, 508-520.

LeTourneau N, Vimal P, Klepacki D, Mankin A, Melman A. (2012) Synthesis and antibacterial activity of desosamine-modified macrolide derivatives. Bioorg. Med. Chem. Lett. 22, 4575-4578.

Locke, J. B., Rahawi, S., LaMarre, J. M., Mankin, A. S., Shaw, K. J. (2012) Genetic environment and stability of cfr in methicillin-resistant Staphylococcus aureus CM05. Antimicrob. Agents Chemother. 56, 332-340.

2011

Kannan, K., Mankin, A. S. (2011) Macrolide antibiotics in the ribosome exit tunnel: species-specific binding and action. Ann. N. Y. Acad. Sci. 1241, 33-47

LaMarre, J. M, Howden, B. P., Mankin, A. S. (2011) Inactivation of the indigenous methyltransferase RlmN in Staphylococcus aureus increases linezolid resistance Antimicrob. Agents Chemother. 55, 2989-2991

LaMarre, J. M., Locke, J.B., Shaw, K.J., Mankin, A. S. (2011) Low fitness cost of the multidrug resistance gene cfr. Antimicrob. Agents Chemother. 55, 3714-3719.

Vázquez-Laslop, N., Mankin, A.S. (2011) Picky nascent peptides do not talk to foreign ribosomes. Proc. Natl. Acad. Sci. USA 108, 5931-5932.

Vazquez-Laslop, N., Klepacki, D., Mulhearn, D.C., Krasnykh, O., Franzblau, S.G, Mankin, A.S. (2011) Role of antibiotic ligand in nascent peptide–dependent ribosome stalling. Proc. Natl. Acad. Sci. USA 108, 10496-10501.

Ramu, H., Vázquez-Laslop, N., Klepacki, D., Dai, Q., Piccirilli, J., Micura, R., Mankin, A.S. (2011) Nascent peptide in the ribosome exit tunnel affects functional properties of the A-site of the peptidyl transferase center. Mol. Cell, 41, 321-330. 

2010

David-Eden, H., Mankin, A.S., Mandel-Gutfreund, Y. (2010) Structural signatures of antibiotic binding sites on the ribosome. Nucl. Acids Res. 38, 5982-5994.

Vázquez-Laslop, N., Ramu, H., Klepacki, D., Kannan, K., Mankin, A.S. (2010) The key function of a conserved and modified rRNA residue in the ribosomal response to the nascent peptide. EMBO J., 29, 3108-3117.

Llano-Sotelo, B., Dunkle, J.A., Klepacki, D., Zhang, W., Fernandes, P., Cate, J.H.D., Mankin, A.S. (2010) Binding and action of CEM-101, a new fluoroketolide antibiotic that inhibits protein synthesis. Anitmicrob. Agents. Chemother. 54, 4961-4970.

Dunkle, J.A., Xiong, L., Mankin, A.S., Cate, J.H.D. (2010) Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action. Proc. Natl. Acad. Sci. USA 107, 17152-17157.

Yan,F., LaMarre, J.M., Röhrich, R., Wiesner, J., Jomaa, H., Mankin, A.S., Galonić-Fujimori, D. (2010) RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA. J. Am. Chem. Soc.132, 3953-3964. 

Auerbach, T., Mermershtain, I., Davidovich, C., Bashan, A., Belousoff, M., Wekselman, I., Zimmerman, E., Xiong, L., Klepacki, D., Arakawa, K., Kinashi, H., Mankin, A.S., Yonath, A. (2010) The structure of ribosome-lankacidin complex reveals ribosomal sites for synergistic antibiotics. Proc. Natl. Acad. Sci. USA 107, 1983-1988.

2009

Llano-Sotelo, B., Kleapcki, D., Mankin A.S. (2009) Selection of small peptides, inhibitors of translation. J. Mol. Biol. 391, 813-819.

Llano-Sotelo, B., Hickerson, R.P, Lancaster, L., Noller, H.F., Mankin A.S. (2009) Fluorescently-labeled ribosomes as a tool for analyzing antibiotic binding. RNA 15, 1597-1604.

Katz, L. and Mankin, A.S. (2009) Macrolides. In Encyclopedia of Microbiology (Third Edition), (Schaechter M., ed.) Elsevier Press, pp. 529-558.

Ramu, H., Mankin, A. and Vazquez-Laslop, N. (2009) Programmed drug-dependent ribosome stalling. Mol. Microbiol. 71, 811-824.

Siibak, T., Peil, L., Xiong, L., Mankin A., Remme, J., Tenson, T. (2009) Erythromycin- and chloramphenicol-induced ribosomal assembly defects are secondary effects of protein synthesis inhibition. Antimicrob. Agents. Chemother. 53, 563-571. 

2008

Mankin, A.S. (2008) Macrolide myths. Curr. Opin. Microbiol. 11, 414-421.

Toh, S,-M., Mankin, A.S. (2008) An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors. J. Mol. Biol. 14, 593-597.

Vazquez-Laslop, N., Thum, C., Mankin, A.S. (2008) Molecular mechanism of drug-dependent ribosome stalling. Mol. Cell 30, 190-202. (Recommended by Faculty of 1000)

Smith, L.K., Mankin, A.S. (2008) Transcriptional and translational control of the mlr operon, which confers resistance to seven classes of protein synthesis inhibitors. Antimicrob. Agents Chemother. 52, 1703-1712.

Bailey, M., Chettiath, T., Mankin, A.S. (2008) Induction of erm(C) expression by ‘non-inducing’ antibiotics. Antimicrob. Agents. Chemother. 52, 866-874.

Toh, S,-M., Xiong, L., Bae, T., Mankin, A.S. (2008) The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA. RNA 14, 98-106.

Mankin, A.S., Polacek, N. (2008) SPARK: A new peptidyl transferase activity assay. Methods Mol. Med. 142, 107-116.

Samant, S., Lee, H., Ghassemi, M., Chen, J., Cook, J.L., Mankin, A.S., Neyfakh, A.A. (2008) Nucleotide biosynthesis is critical for growth of bacteria in human blood. PLoS Pathogen 4, e37.

2007

Yassin, A., Mankin, A.S. (2007) Potential new antibiotic sites in the ribosome revealed by deleterious mutations in RNA of the large ribosomal subunit. J. Biol. Chem., 282, 24329-24342.

Leach, K.L., Swaney, S.M., Colca, J.R., McDonald, W.G., BlinnJ.R., Thomasco, L.M., Gadwood, R.C., Shinabarger, D. , Xiong, L., Mankin, A.S. (2007) The site of action of oxazolidinone antibiotics in living bacteria and in human mitochondria. Mol. Cell, 26, 393-402.

Toh, S.-M, Xiong, L., Arias, C.A., Villegas, M.V., Lolans, K., Quinn, J., Mankin, A.S. (2007) Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid. Mol. Microbiol. 64, 1506-1514.

2006

Mankin, A.S. (2006) Antibiotic blocks mRNA path on the ribosome. Nat. Struct. Mol. Biol. 13, 858-860.

Tenson, T., Mankin, A.S. (2006) Antibiotics and the ribosome. Mol. Microbiol. 59,1664-1677.

Sherman, D., Xiong, L., Mankin, A.S., Melman, A. (2006) Synthesis and biological investigation of new 4''-malonyl tethered derivatives of erythromycin and clarithromycin. Bioorg. Med. Chem. Lett. 16, 1506-1509.

Mankin, A.S. (2006) Nascent peptide in the birth canal of the ribosome. Trends. Biochem. Sci. 31, 11-13, 2006.

2005

Maguire, B.A., Beniaminov, A.D., Ramu, H., Mankin, A.S., Zimmermann, R.A. (2005) A protein component at the heart of an RNA machine: the importance of protein L27 for the function of the bacterial ribosome. Mol. Cell 20, 427-435. 

Yassin, A., Fredrick, K., Mankin, A.S. (2005) Deleterious mutations in small subunit ribosomal RNA identify functional sites and potential targets for antibiotics. Proc. Natl. Acad. Sci. USA 102, 16620-16625.

Polacek, N., Mankin, A.S. (2005) The ribosomal peptidyl transferase center: structure, function, evolution, inhibition. Crit. Revs. Biochem. Molec. Biol. 40, 285-311.

Beringer, M., Bruell, C., Xiong, L., Pfister, P., Bieling, P., Katunin, V.I., Mankin, A.S., Bottger, E.C., Rodnina, M.V. (2005) Essential mechanisms in the catalysis of peptide bond formation on the ribosome. J. Biol. Chem. 280, 36065-36072.

Erlacher, M.D., Lang, K., Shankaran, N., Wotzel, B., Huttenhofer, A., Micura, R., Mankin, A.S., Polacek, N. (2005) Chemical engineering of the peptidyl transferase center reveals an important role of the 2'-hydroxyl group of A2451. Nucleic Acids Res. 33, 1618-1627. 

Xiong, L., Korkhin, Y., Mankin, A.S. (2005) Binding site of bridged ketolides in the Escherichia coli ribosome. Antimicrob. Agents Chemother. 49, 281-288.

2004

Vimberg, V., Xiong, L., Bailey, M., Tenson, T., Mankin, A.S. (2004) Peptide-mediated macrolide resistance reveals possible specific interactions in the nascent peptide exit tunnel. Mol. Microbiol. 54, 376-385.

2003

Colca, J.R., McDonald, W.G., Waldon, D.J., Thomasco, L.M., Gadwood,, R.C., Lund, E.T., Cavey, G.S., Mathews, W.R., Adams, L.D., Cecil, E.T., Pearson, J.D., Bock, J.H., Mott, J.E., Shinabarger, D.L., Xiong, L., Mankin, A.S. (2003) Crosslinking in the living cell locates the site of action of oxazolidinone antibiotics. J. Biol. Chem. 278, 21972-21979.

Polacek, N., Gomez, M.J., Ito, K. Xiong, L., Nakamura, Y., Mankin, A.S. (2003) The critical role of the universally conserved A2602 of 23S ribosomal RNA in the release of the nascent peptide during translation termination. Mol. Cell, 11, 103-112.

Ruggero, K.A., Schroeder, L.K., Schreckenberger, P.C., Mankin, A.S., Quinn, J.P. (2003) Nosocomial superinfections due to linezolid-resistant Enterococcus faecalis: evidence for a gene dosage effect on linezolid MICs. Diagn. Microbiol. Infect. Dis. 47, 511-513.

Gaynor, M., Mankin, A.S. (2003) Macrolide antibiotics: binding site, mechanism of action, resistance. Curr Top Med Chem. 3, 949-961.

2002

Sander, P., Belova, L., Kidan, Y.G., Pfister, P., Mankin, A.S., Böttger, E.C. (2002) Ribosomal and non-ribosomal resistance to oxazolidinones: species-specific idiosyncrasy of ribosomal alterations. Mol. Microbiol. 46, 1295-1304.

Polacek, N., Swaney, S., Shinabarger, D., Mankin, A.S. (2002) SPARK-a novel method to monitor ribosomal peptidyl transferase activity. Biochemistry 41, 11602-11610.

2001

Garza-Ramos, G., Xiong, L., Zhong, P., Mankin, A.S. (2001) Binding site of macrolide antibiotics on the ribosome: new resistance mutation identifies a specific interaction of ketolides with rRNA. J. Bacteriol. 183, 6898-6907.

Tenson, T., Mankin, A.S. (2001) Short peptides conferring resistance to macrolide antibiotics. Peptides 22, 1661-1668.

Polacek, N., Gaynor, M., Yassin, A., Mankin, A.S. (2001) Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide. Nature 411, 498-501.

Xiong, L., Polacek, Sander, P., Boettger, E.C., Mankin, A.S. (2001) pKa of adenine 2451 in the ribosomal peptidyl transferase center remains elusive. RNA 7, 1365-1369.

Mankin, A.S. (2001) Ribosomal antibiotics. Mol. Biologia 35, 509-520.

Belova, L., Tenson , T., Xiong, L., McNicholas, P.M., Mankin, A.S. (2001) A novel site of antibiotic action in the ribosome: Interaction of evernimicin with the large ribosomal subunit. Proc. Natl. Acad. Sci. USA 98, 3726–3731.

Mann, P. A., Xiong, L., Mankin, A. S., Chau, A. S., Mendrick, C. A., Najarian, D. J., Cramer, C. A., Loebenberg, D., Coates, E., Murgolo, N. J., Aarestrup, F. M., Goering, R., Black, T. A., Hare, R. S., and McNicholas, P.M. (2001) EmtA, a rRNA methylase conferring high-level evernimicin resistance. Mol. Microbiol. 41:1349-1356.

Bocchetta, M., Xiong, L., Shah, S., Mankin, A.S. (2001) Interactions between 23S rRNA and tRNA in the ribosomal E site. RNA, 7, 54-63.

2000

Xiong, L., Kloss, P., Douthwaite, S., Andersen, N.M., Swaney, S., Shinabarger, D.L., Mankin, A.S. (2000) Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action. J. Bacteriol. 182, 5325-5331.

Velichutina, I.V., Dresios, J., Hong, J.Y., Li, C., Mankin, A., Synetos, D., Liebman, S.W. (2000) Mutations in helix 27 of the yeast Saccharomyces cerevisiae 18S rRNA affect the function of the decoding center of the ribosome. RNA, 6, 1174-1184.

Khaitovich, P., and Mankin, A. S. (2000) Reconstitution of the 50S subunit with in vitro-transcribed 23S rRNA: a new tool for studying peptidyl transferase. In The Ribosome: Structure, Function, Antibiotics, and Cellular Interactions (Garrett, R.A., Douthwaite, S.R., Liljas, A., Matheson, A.T., Moore, P.B. and Noller, H.F., eds). ASM Press, Washington, D.C., pp. 229-243.

Novikova S.I., Bushueva A.M., Trachuk L.A., Konstantinova G.E., Serkina A.V., Hoischen C., Gumpert J., Chestukhina G.G., Mankin A., Shevelev A.B. (2000) Introduction of a mini-gene encoding a five-amino acid peptide confers erythromycin resistance on Bacillus subtilis and provides temporary erythromycin protection in Proteus mirabilis. FEMS Microbiol Lett., 182, 213-218.

1999

Mankin, A. S. and Liebman, S.W (1999) Baby, don’t stop! Nature Genet. 23, 8-10.

Kloss, P., Xiong, L., Shinabarger, D. and Mankin, A.S. (1999) Resistance mutations in 23S rRNA identify the site of action of protein synthesis inhibitor, linezolid, in the ribosomal peptidyl transferase center. J. Mol. Biol. 294, 93-101.

Chernyaeva, N. S., Murgola, E. J. and Mankin, A. S. (1999) Suppression of nonsense mutations induced by expression of an RNA complementary to a conserved segment of 23S rRNA. J. Bacteriol. 181, 5257-5262.

Khaitovich, P., and Mankin, A. S. (1999) Effect of antibiotics on large ribosomal subunit assembly reveals possible function of 5S rRNA. J. Mol. Biol., 291, 1025-1034.

Tenson, T., Herrera, J. V., Kloss, P., Guarneros, G., and Mankin, A. S. (1999) Inhibition of translation and cell growth by minigene expression. J. Bacteriol. 181, 1617-1622.

Khaitovich, P., Tenson, T., Mankin, A. S. and Green, R. (1999) Peptidyl transferase activity catalyzed by protein-free 23S ribosomal RNA remains elusive. RNA 5, 605-608.

Khaitovich, P., Tenson, T., Kloss, P. and Mankin, A. S. (1999) Reconstitution of functionally active Thermus aquaticus large ribosomal subunits with in vitro-transcribed rRNA. Biochemistry 38, 1780-1788.

Khaitovich, P., Mankin, A. S., Green, R., Lancaster, L. and Noller, H. F. (1999) Characterization of functionally active subribosomal particles from Thermus aquaticus. Proc. Natl. Acad. Sci. USA 96, 85-90.

Xiong, L., Shah, S., Mauvais, P. and Mankin, A. S. (1999) A ketolide resistance mutation in domain II of 23S rRNA reveals proximity of hairpin 35 to the peptidyl transferase center. Mol. Microbiol. 31, 633-639.

1998

Tripathi, S., Kloss, P.S. and Mankin, A. S. (1998) Ketolide resistance conferred by short peptides. J. Biol. Chem. 273, 20073-20077.

Arkov, A., Mankin, A. S. and Murgola, E.J. (1998) An rRNA fragment and its antisense can alter decoding of genetic information. J. Bacteriol. 180, 2744-2748.

Porse, B. T., Leviev, I., Mankin, A. S. and Garrett, R. A. (1998) The antibiotic thiostrepton inhibits a functional transition within protein L11 at the ribosomal GTPase centre. J. Mol. Biol. 276, 391-404.

Bocchetta, M., Xiong, L. and Mankin, A. S. (1998) 23S rRNA positions essential for tRNA binding in ribosomal functional sites. Proc. Natl. Acad. Sci. USA 95, 3525-2530.

1997

Mankin, A. S. (1997) Pactamycin resistance mutations in functional sites of 16S rRNA. J. Mol. Biol. 274, 8-15.

Tenson, T., Xiong, L., Kloss, P. and Mankin, A. S. (1997) Erythromycin resistance peptides selected from random peptide libraries. J. Biol. Chem. 272, 17425-17430.

1996

Tan, G. T., DeBlasio, A. and Mankin, A. S. (1996) Mutations in the peptidyl transferase center of 23S rRNA reveal site of action of sparsomycin, a universal inhibitor of translation. J. Mol. Biol. 261, 222-230.

Dam, M, Douthwaite, S., Tenson, T. and Mankin, A. S. (1996) Mutations in domain II of 23S rRNA facilitate translation of a 23S rRNA-encoded pentapeptide conferring erythromycin resistance. J. Mol. Biol. 259, 1-6.

Tenson, T., DeBlasio, A. and Mankin, A. S. (1996) A functional peptide encoded in the Escherichia coli 23S rRNA. Proc. Natl. Acad. Sci. USA 93, 5641-5646.

1995

Tenson, T. and Mankin, A. S. (1995) Comparison of functional peptide encoded in the Escherichia coli 23S rRNA with other peptides involved in cis-regulation of translation. Biochem. Cell Biol. 73, 1061-1070.

Mankin, A. S. (1995) Selection for spontaneous and engineered mutations in the rRNA genes in halophilic Archaea. In Archaea, A Laboratory Manual (Robb, F.T. et al., eds). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, USA, pp.209-216.

1994

Mankin, A. S., Leviev, I. G. and Garrett, R. A. (1994) Cross-hypersensitivity effects of mutations in 23S rRNA yield insight into aminoacyl-tRNA binding. J. Mol. Biol. 244, 151-157.

Leviev, I. G., Rodriguez-Fonseca, C., Phan, H., Garrett, R. A., Noller, H. F. and Mankin, A. S. (1994) A conserved secondary structure motif in 23S rRNA defines the site of interaction of amicetin, a universal inhibitor of peptide bond formation. EMBO J. 13, 1682-1686.

Mankin, A. S., Derckacheva, N. I., Leviev, I. G., Kagramanova, V. K., Forterre, P. and Garrett, R. A. (1994) Stable maintenance in Halobacteria of plasmids harboring rDNA. Syst. Appl. Microbiol. 16, 672-679.

1993

Akhmanova A. S., Kagramanova, V. K. and Mankin A. S. (1993) Sequence heterogeneity of small multicopy plasmids in halophilic archaea. J. Bacteriol. 175, 1081-1086.